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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
18/04/2023 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VASQUEZ, D. D. N.; PINHEIRO, D. H.; TEIXEIRA, L. A.; MOREIRA-PINTO, C. E.; MACEDO, L. L. P. de; SALLES-FILHO, A. L. O.; SILVA, M. C. M. da; LOURENCO, I. T.; MORGANTE, C. V.; SILVA, L. P. da; SA, M. F. G. de. |
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Afiliação: |
DANIEL D. N. VASQUEZ, Catholic University of Brasília; DANIELE H. PINHEIRO; LAYS A. TEIXEIRA, Catholic University of Brasília; CLIDIA E. MOREIRA-PINTO; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ALVARO L. O. SALLES-FILHO, Federal University of Paraná; MARIA CRISTINA MATTAR DA SILVA, Cenargen; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; LUCIANO PAULINO DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Simultaneous silencing of juvenile hormone metabolism genes through RNAi interrupts metamorphosis in the cotton boll weevil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Frontiers in Molecular Biosciences, v. 10, 2023. |
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DOI: |
https://doi.org/10.3389/fmolb.2023.1073721 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Leonardo L. P. Macedo; Maria C. M. Silva; Isabela T. Lourenço-Tessutti; Luciano P. Silva; Maria F. Grossi-de-Sa. |
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Conteúdo: |
The cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the injection of naked dsRNAs or dsRNAs complexed with PEI (500 ng) on CBW survival and phenotypes. Overall, the gene expression analysis showed that the treatments with PEI targeting either a single gene or multiple genes induced greater gene silencing than naked dsRNA (~60%). In addition, the injection of dsJHEH/JHDK, either naked or complexed with PEI, significantly affected CBW survival (18% for PEI nanoparticles and 47% for naked dsRNA) and metamorphosis. Phenotypic alterations, such as uncompleted pupation or malformed pupae, suggested that JHEH and JHDK are involved in developmental regulation. Moreover, CBW larvae treated with dsJHEH/JHDK + PEI (1,000 ng/g) exhibited significantly lower survival rate (55%) than those that MenosThe cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the inj... Mostrar Tudo |
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Palavras-Chave: |
Bicudo do algodoeiro; Juvenile diol kinase; Juvenile hormone epoxide hydrolase; Polyethylenimine; Pragas do algodoeiro. |
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Thesagro: |
Algodão; Anthonomus Grandis; Inseto; Praga. |
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Thesaurus Nal: |
Chitosan; Cotton; Insect control; Insect pests. |
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Categoria do assunto: |
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O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153217/1/fmolb-10-1073721.pdf
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Marc: |
LEADER 03612naa a2200421 a 4500
001 2153271
005 2023-05-25
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fmolb.2023.1073721$2DOI
100 1 $aVASQUEZ, D. D. N.
245 $aSimultaneous silencing of juvenile hormone metabolism genes through RNAi interrupts metamorphosis in the cotton boll weevil.$h[electronic resource]
260 $c2023
500 $aNa publicação: Leonardo L. P. Macedo; Maria C. M. Silva; Isabela T. Lourenço-Tessutti; Luciano P. Silva; Maria F. Grossi-de-Sa.
520 $aThe cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the injection of naked dsRNAs or dsRNAs complexed with PEI (500 ng) on CBW survival and phenotypes. Overall, the gene expression analysis showed that the treatments with PEI targeting either a single gene or multiple genes induced greater gene silencing than naked dsRNA (~60%). In addition, the injection of dsJHEH/JHDK, either naked or complexed with PEI, significantly affected CBW survival (18% for PEI nanoparticles and 47% for naked dsRNA) and metamorphosis. Phenotypic alterations, such as uncompleted pupation or malformed pupae, suggested that JHEH and JHDK are involved in developmental regulation. Moreover, CBW larvae treated with dsJHEH/JHDK + PEI (1,000 ng/g) exhibited significantly lower survival rate (55%) than those that
650 $aChitosan
650 $aCotton
650 $aInsect control
650 $aInsect pests
650 $aAlgodão
650 $aAnthonomus Grandis
650 $aInseto
650 $aPraga
653 $aBicudo do algodoeiro
653 $aJuvenile diol kinase
653 $aJuvenile hormone epoxide hydrolase
653 $aPolyethylenimine
653 $aPragas do algodoeiro
700 1 $aPINHEIRO, D. H.
700 1 $aTEIXEIRA, L. A.
700 1 $aMOREIRA-PINTO, C. E.
700 1 $aMACEDO, L. L. P. de
700 1 $aSALLES-FILHO, A. L. O.
700 1 $aSILVA, M. C. M. da
700 1 $aLOURENCO, I. T.
700 1 $aMORGANTE, C. V.
700 1 $aSILVA, L. P. da
700 1 $aSA, M. F. G. de
773 $tFrontiers in Molecular Biosciences$gv. 10, 2023.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
28/02/2011 |
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Data da última atualização: |
21/08/2017 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
CRUZ, I.; FIGUEIREDO, M. de L. C.; SILVA, R. B. da. |
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Afiliação: |
IVAN CRUZ, CNPMS; MARIA DE LOURDES CORREA FIGUEIREDO, BOLSISTA; RAFAEL BRAGA DA SILVA, BOLSISTA. |
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Título: |
Monitoramento de adultos de Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) e Diatraea saccharalis (Fabricius) (Lepidoptera: Pyralidae) em algumas regiões produtoras de milho no Brasil. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Sete Lagoas: Embrapa Milho e Sorgo, 2010. |
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Páginas: |
42 p. |
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Série: |
(Embrapa Milho e Sorgo. Documentos, 93). |
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Idioma: |
Português |
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Conteúdo: |
Foram monitorados insetos adultos de Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), lagarta-do-cartucho, e Diatraea saccharalis (Fabricius) (Lepidoptera: Pyralidae), broca-da-cana-deaçúcar, utilizando armadilhas contendo como atraente o feromônio sexual sintético e fêmeas virgens, para as respectivas pragas. O monitoramento foi realizado em diferentes regiões produtoras de milho no Brasil, abrangendo os estados de Goiás, Mato Grosso, Minas Gerais, Paraná e São Paulo. De maneira geral, a ocorrência de ambas as pragas foi significativamente alta nas regiões amostradas, sendo que a maior incidência foi observada nos primeiros trinta dias após a emergência da planta. A presença constante da mariposa de D. saccharalis em diferentes áreas de produção no Brasil sugere que tal praga possa estar reduzindo a produtividade de milho e, portanto, deve merecer estudos adicionais para se estabelecer um programa de manejo. |
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Thesagro: |
Inseto; Praga de planta; Zea mays. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/28766/1/Doc-93.pdf
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Marc: |
LEADER 01644nam a2200193 a 4500
001 1879462
005 2017-08-21
008 2010 bl uuuu u0uu1 u #d
100 1 $aCRUZ, I.
245 $aMonitoramento de adultos de Spodoptera frugiperda (J. E. Smith) (Lepidoptera$bNoctuidae) e Diatraea saccharalis (Fabricius) (Lepidoptera: Pyralidae) em algumas regiões produtoras de milho no Brasil.$h[electronic resource]
260 $aSete Lagoas: Embrapa Milho e Sorgo$c2010
300 $a42 p.
490 $a(Embrapa Milho e Sorgo. Documentos, 93).
520 $aForam monitorados insetos adultos de Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), lagarta-do-cartucho, e Diatraea saccharalis (Fabricius) (Lepidoptera: Pyralidae), broca-da-cana-deaçúcar, utilizando armadilhas contendo como atraente o feromônio sexual sintético e fêmeas virgens, para as respectivas pragas. O monitoramento foi realizado em diferentes regiões produtoras de milho no Brasil, abrangendo os estados de Goiás, Mato Grosso, Minas Gerais, Paraná e São Paulo. De maneira geral, a ocorrência de ambas as pragas foi significativamente alta nas regiões amostradas, sendo que a maior incidência foi observada nos primeiros trinta dias após a emergência da planta. A presença constante da mariposa de D. saccharalis em diferentes áreas de produção no Brasil sugere que tal praga possa estar reduzindo a produtividade de milho e, portanto, deve merecer estudos adicionais para se estabelecer um programa de manejo.
650 $aInseto
650 $aPraga de planta
650 $aZea mays
700 1 $aFIGUEIREDO, M. de L. C.
700 1 $aSILVA, R. B. da
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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