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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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Data corrente: |
22/01/2024 |
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Data da última atualização: |
02/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MAGARELLI, G.; SILVA, J. G. da; RIBEIRO, C. L.; FREITAS, T. V. de; RODRIGUES, M. A.; GIL, E. de S.; MARRACCINI, P.; SOUZA, J. R. de; CASTRO, C. S. P. de; BEMQUERER, M. P. |
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Afiliação: |
GABRIELLA MAGARELLI, Cenargen; JONATAS GOMES DA SILVA, UNIVERSIDADE FEDERAL DO OESTE DA BAHIA; CAROLINE LUCHTENBERG RIBEIRO; THIAGO VIANA DE FREITAS; MAGALI APARECIDA RODRIGUES, UNIVERSIDADE DE SAO PAULO; ERIC DE SOUZA GIL, UNIVERSIDADE FEDERAL DE GOIAS; PIERRE MARRACCINI, CIRAD; JURANDIR RODRIGUES DE SOUZA, UNIVERSIDADE DE BRASÍLIA; CLARISSA SILVA PIRES DE CASTRO, GIS; MARCELO PORTO BEMQUERER, CNPGL. |
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Título: |
A voltammetric peptide biosensor for Cu2+ metal ion quantification in coffee seeds. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Journal of Inorganic Biochemistry, v. 251, 112441, 2024. |
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DOI: |
https://doi.org/10.1016/j.jinorgbio.2023.112441 |
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Idioma: |
Inglês |
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Conteúdo: |
A prion-derived copper(II)-binding peptide was assembled onto a gold electrode for the building of a voltammetric biosensor for measuring the Cu2+ metal ion in biological samples. The chosen sequence was HCVNITKQHTVTTTT-NH2, with an appended cysteine residue for binding to the gold surface as a self-assembled monolayer and a histidine residue as the anchorage point for copper(II) complexation. The biosensor showed a linear range of 10-7 to 10-6 M with an 8.0 x 10-8 M detection limit and a 1.0 x 10-7 M quantification limit, with good precision, trueness, and absence of matrix effect. The quantification of Cu2+ was performed in the presence of other transition metal ions, such as Zn2+, Cd2+, Fe2+, or Ni2+, which indicates the excellent selectivity of the biosensor. When the modified electrode was applied for measuring copper(II) in calcined coffee seeds, a difference in copper amount was observed between two Coffea arabica cultivars that were submitted to a treatment with a copper-based antifungal, showing the applicability of the biosensor in the agricultural field. |
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Palavras-Chave: |
Biossensor peptídico; Coffee seeds; Peptide biosensor; Prion peptide; Voltametria; Voltammetry. |
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Thesagro: |
Café; Peptídeo; Semente. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02087naa a2200349 a 4500
001 2161562
005 2024-02-02
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.jinorgbio.2023.112441$2DOI
100 1 $aMAGARELLI, G.
245 $aA voltammetric peptide biosensor for Cu2+ metal ion quantification in coffee seeds.$h[electronic resource]
260 $c2024
520 $aA prion-derived copper(II)-binding peptide was assembled onto a gold electrode for the building of a voltammetric biosensor for measuring the Cu2+ metal ion in biological samples. The chosen sequence was HCVNITKQHTVTTTT-NH2, with an appended cysteine residue for binding to the gold surface as a self-assembled monolayer and a histidine residue as the anchorage point for copper(II) complexation. The biosensor showed a linear range of 10-7 to 10-6 M with an 8.0 x 10-8 M detection limit and a 1.0 x 10-7 M quantification limit, with good precision, trueness, and absence of matrix effect. The quantification of Cu2+ was performed in the presence of other transition metal ions, such as Zn2+, Cd2+, Fe2+, or Ni2+, which indicates the excellent selectivity of the biosensor. When the modified electrode was applied for measuring copper(II) in calcined coffee seeds, a difference in copper amount was observed between two Coffea arabica cultivars that were submitted to a treatment with a copper-based antifungal, showing the applicability of the biosensor in the agricultural field.
650 $aCafé
650 $aPeptídeo
650 $aSemente
653 $aBiossensor peptídico
653 $aCoffee seeds
653 $aPeptide biosensor
653 $aPrion peptide
653 $aVoltametria
653 $aVoltammetry
700 1 $aSILVA, J. G. da
700 1 $aRIBEIRO, C. L.
700 1 $aFREITAS, T. V. de
700 1 $aRODRIGUES, M. A.
700 1 $aGIL, E. de S.
700 1 $aMARRACCINI, P.
700 1 $aSOUZA, J. R. de
700 1 $aCASTRO, C. S. P. de
700 1 $aBEMQUERER, M. P.
773 $tJournal of Inorganic Biochemistry$gv. 251, 112441, 2024.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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| 1. |  | SOUSA, M. P. S.; CAETANO, G. B.; MACIEL, A. T. da S.; SILVA, N. T. D. da; SOUSA, M. do S. R.; FREITAS, D. G. de. Interação entre pesquisa e educação básica por meio de programa de rádio web escolar. In: JORNADA CIENTÍFICA DA EMBRAPA AMAPÁ, 5., 2019, Macapá. Resumos... Macapá: Embrapa Amapá, 2020. Editoras técnicas: Jamile da Costa Araújo e Ana Cláudia Lira-Guedes.| Tipo: Resumo em Anais de Congresso |
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