Data/hora: 05/06/2024 08:27:51

Biblioteca(s): | Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 10/02/2017
Data da última atualização: | 29/03/2023
Autoria: | LOBO, F. P.; CINTRA, L. C.; VARELA, E. S.; ALVES, A. L.; SILVA, N. M. A. da; IANELLA, P.; PAIVA, S. R.; CAETANO, A. R.
Afiliação: | FRANCISCO PEREIRA LOBO, CNPTIA; LEANDRO CARRIJO CINTRA, CNPTIA; EDUARDO SOUSA VARELA, CNPASA; ANDERSON LUIS ALVES, CNPASA; NAIARA MILAGRES AUGUSTO DA SILVA, CENARGEN; PATRICIA IANELLA, CENARGEN; SAMUEL REZENDE PAIVA, SRI; ALEXANDRE RODRIGUES CAETANO, CENARGEN.
Título: | Genome Sequencing and de novo assembly of the South American Tiger Catfish (Pseudoplatystoma punctifer).
Ano de publicação: | 2016
Fonte/Imprenta: | In: PLANT & ANIMAL GENOME CONFERENCE, 24., 2016, San Diego, CA. [Abstracts...]. San Diego: [s. n.], 2016.
Páginas: | Não paginado.
Idioma: | Inglês
Notas: | PAG 2016. Pôster P0481.
Conteúdo: | The South American Tiger Catfish (Pseudoplatystoma punctifer) is a freshwater fish species naturally found in the Amazon river basin with an important role in local community and commercial fishing. Recent efforts to domesticate and raise the species in aquaculture systems has led to >10-fold production increases in the last decade. Current production levels are >15,000mt/year. Recently stablished initiatives to structure genetic improvement programs for increasing productivity-associated traits could greatly benefit from using genomic tools for broodstock management and assisted genetic evaluations and breeding. Multiple shotgun libraries with two different insert sizes and multiple Nextera mate pair libraries with three different sizes were sequenced (2x160bps) with Illumina HiSeq2000 technology. A total of 124.8Gbp quality-filtered nucleotides were sequenced which amount to 85x mean genome coverage, considering previously published information (C-value = 1.12pg = 1.095Gbp). Initial sequence assembly was performed with SOAPdenovo and generated 686.171 scaffolds spanning 1.41 Gbp (N50 scaffold length: 908Kbp, L50 scaffold count: 420). Gene model prediction is underway with MAKER2 using as extrinsic evidence protein and EST data from phylogenetically related taxa. This represents the first report of a draft genome sequence for this species, which will be a valuable resource for basic biology studies, and marker detection/selection for use in genetic improvement and resource conservation activities.
Palavras-Chave: | Bioinformática; Genômica; Sequenciamento de genoma.
Thesaurus Nal: | Bioinformatics; Genome assembly; Genomics; Sequence analysis.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/140246/1/PAG-p0481.pdf
Registro original: | Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)
Exemplares: | CENARGEN - (36630-1) - UPC - PC - DD - SP 21028a -  - SP 21028a - DIS