| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Meio Norte / UEP-Parnaíba. |
|
Data corrente: |
28/07/1995 |
|
Data da última atualização: |
28/07/1995 |
|
Autoria: |
NUGENT, M. E.; PRIMROSE, S. B.; TACON, W. C. A. |
|
Afiliação: |
Department of Microbiology and Process Research, Searle Research and Development. |
|
Título: |
The stability of recombinant DNA. |
|
Ano de publicação: |
0 |
|
Fonte/Imprenta: |
s.n.t. |
|
Páginas: |
p.271-285 |
|
Idioma: |
Inglês |
|
Notas: |
Separata. |
|
Conteúdo: |
A high copy number mutant of a trp expression plasmid has been used toincrease the expression of the cloned gene urogastrone. This mutation results in up to an eightfold increase in copy number over pBR322. However, when the mutation was cloned into a urogastrone-producing plasmid, only 5% of the resulting recombinants were of the right construction (pMN38-2); 90% had undergone deletions and 5% ha insertions. Attemptwas made to increase urogastrone expression further by cloning a triple trp promoter fragment into pMN38-2. Although there were problems dueto structural instability 10% of recombinants had the correct structure (pMN37-1). However, this plasmid did not lead to any increase in urogastrone production over pMN38-2. In expression studies using there + E. coli K12 HW27 pMN37-1, instability was observed due to homologous recombination between promoter units whereas in the rec strain HB101 drops in copy number were observed. A plasmid, pWT600, was constructed which carries a fusion between a beta-interferon gene and a beta-galactosidase gene. The expression of this gene fusion was under trp promoter control. Cells carrying pWT600 rapidly threw off mutants with decreased expression of this fusion. This was due either to a chromosomal mutation resulting in a 10-fold drop in copy number or to insertions of IS10 from the chromosome into pWT600. From the observations on structural instability, it is important to check the structure and copy number of plasmids during studies designed to maximize expression. MenosA high copy number mutant of a trp expression plasmid has been used toincrease the expression of the cloned gene urogastrone. This mutation results in up to an eightfold increase in copy number over pBR322. However, when the mutation was cloned into a urogastrone-producing plasmid, only 5% of the resulting recombinants were of the right construction (pMN38-2); 90% had undergone deletions and 5% ha insertions. Attemptwas made to increase urogastrone expression further by cloning a triple trp promoter fragment into pMN38-2. Although there were problems dueto structural instability 10% of recombinants had the correct structure (pMN37-1). However, this plasmid did not lead to any increase in urogastrone production over pMN38-2. In expression studies using there + E. coli K12 HW27 pMN37-1, instability was observed due to homologous recombination between promoter units whereas in the rec strain HB101 drops in copy number were observed. A plasmid, pWT600, was constructed which carries a fusion between a beta-interferon gene and a beta-galactosidase gene. The expression of this gene fusion was under trp promoter control. Cells carrying pWT600 rapidly threw off mutants with decreased expression of this fusion. This was due either to a chromosomal mutation resulting in a 10-fold drop in copy number or to insertions of IS10 from the chromosome into pWT600. From the observations on structural instability, it is important to check the structure and copy number of plasmids during studies ... Mostrar Tudo |
|
Palavras-Chave: |
Clonagem genica; DNA recombinante; Estabilidade; Expressao plasmidial. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02038naa a2200217 a 4500
001 1074997
005 1995-07-28
008 bl --- 0-- u #d
100 1 $aNUGENT, M. E.
245 $aThe stability of recombinant DNA.
260 $c0
300 $ap.271-285
500 $aSeparata.
520 $aA high copy number mutant of a trp expression plasmid has been used toincrease the expression of the cloned gene urogastrone. This mutation results in up to an eightfold increase in copy number over pBR322. However, when the mutation was cloned into a urogastrone-producing plasmid, only 5% of the resulting recombinants were of the right construction (pMN38-2); 90% had undergone deletions and 5% ha insertions. Attemptwas made to increase urogastrone expression further by cloning a triple trp promoter fragment into pMN38-2. Although there were problems dueto structural instability 10% of recombinants had the correct structure (pMN37-1). However, this plasmid did not lead to any increase in urogastrone production over pMN38-2. In expression studies using there + E. coli K12 HW27 pMN37-1, instability was observed due to homologous recombination between promoter units whereas in the rec strain HB101 drops in copy number were observed. A plasmid, pWT600, was constructed which carries a fusion between a beta-interferon gene and a beta-galactosidase gene. The expression of this gene fusion was under trp promoter control. Cells carrying pWT600 rapidly threw off mutants with decreased expression of this fusion. This was due either to a chromosomal mutation resulting in a 10-fold drop in copy number or to insertions of IS10 from the chromosome into pWT600. From the observations on structural instability, it is important to check the structure and copy number of plasmids during studies designed to maximize expression.
653 $aClonagem genica
653 $aDNA recombinante
653 $aEstabilidade
653 $aExpressao plasmidial
700 1 $aPRIMROSE, S. B.
700 1 $aTACON, W. C. A.
773 $ts.n.t.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Semiárido; Embrapa Solos. |
|
Data corrente: |
23/11/2007 |
|
Data da última atualização: |
22/02/2019 |
|
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
|
Autoria: |
ANJOS, J. B. dos; CAVALCANTI, N. de B.; BRITO, L. T. de L.; SILVA, M. S. L. da. |
|
Afiliação: |
JOSE BARBOSA DOS ANJOS, CPATSA; NILTON DE BRITO CAVALCANTI, CPATSA; LUIZA TEIXEIRA DE LIMA BRITO, CPATSA; MARIA SONIA LOPES DA SILVA, CNPS. |
|
Título: |
Captação "in situ": água de chuva para produção de alimentos. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
In: BRITO, L. T. de L.; MOURA, M. S. B. de; GAMA, G. F. B. (Ed.). Potencialidades da água de chuva no semi-árido brasileiro. Petrolina: Embrapa Semi-Árido, 2007. |
|
Páginas: |
cap. 7, p.141-155. |
|
Idioma: |
Português |
|
Conteúdo: |
Captação de água de chuva in situ é uma maneira de preparar o solo para o plantio de culturas, principalmente anuas, como milho, feijão, mandioca, exploradas em condições dependentes de chuva. |
|
Palavras-Chave: |
Água de chuva; Captação; Captação de água de chuva; In situ. |
|
Thesagro: |
Alimento; Produção de Alimentos; Solo; Tecnologia. |
|
Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPATSA/36540/1/OPB1523.pdf
|
|
Marc: |
LEADER 01038naa a2200265 a 4500
001 1158513
005 2019-02-22
008 2007 bl uuuu u00u1 u #d
100 1 $aANJOS, J. B. dos
245 $aCaptação "in situ"$bágua de chuva para produção de alimentos.
260 $c2007
300 $acap. 7, p.141-155.
520 $aCaptação de água de chuva in situ é uma maneira de preparar o solo para o plantio de culturas, principalmente anuas, como milho, feijão, mandioca, exploradas em condições dependentes de chuva.
650 $aAlimento
650 $aProdução de Alimentos
650 $aSolo
650 $aTecnologia
653 $aÁgua de chuva
653 $aCaptação
653 $aCaptação de água de chuva
653 $aIn situ
700 1 $aCAVALCANTI, N. de B.
700 1 $aBRITO, L. T. de L.
700 1 $aSILVA, M. S. L. da
773 $tIn: BRITO, L. T. de L.; MOURA, M. S. B. de; GAMA, G. F. B. (Ed.). Potencialidades da água de chuva no semi-árido brasileiro. Petrolina: Embrapa Semi-Árido, 2007.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
