Data/hora: 10/06/2024 01:12:47

Biblioteca(s): | Embrapa Gado de Corte; Embrapa Gado de Leite.
Data corrente: | 27/12/2019
Data da última atualização: | 27/12/2019
Autoria: | SOUZA, I. I. F. de; RODRIGUES, R. de A.; JORGE, K. S. G.; SILVA, M. R.; LILENBAUM, W.; VIDAL, C. E. S.; ETGES, R. N.; KOSTOVIC, M.; ARAUJO, F. R.
Afiliação: | Ingrid Ieda Fernando de Souza, Universidade Federal de Mato Grosso do Sul - UFMS; Rudielle de Arruda Rodrigues, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina, Veterinaria e Zootecnia- FMVZ; Kláudia Santos Gonçalves Jorge, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina, Veterinaria e Zootecnia- FMVZ; MARCIO ROBERTO SILVA, CNPGL; Walter Lilenbaum, Universidade Federal Fluminense - UFLU/Laboratório de Bacteriologia e Veterinária; Carlos Eugênio Soto Vidal; Rodrigo Nestor Etges; Miladin Kostovic; FLABIO RIBEIRO DE ARAUJO, CNPGC.
Título: | ELISA using a recombinant chimera of ESAT-6/MPB70/MPB83 for Mycobacterium bovis diagnosis in naturally infected cattle.
Ano de publicação: | 2019
Fonte/Imprenta: | The Journal Veterinary Medical Science, v. 81, n. 1, p. 9-14, 2019.
Idioma: | Inglês
Conteúdo: | Bovine tuberculosis (bTB) control programs generally rely on intradermal tuberculin tests for the antemortem diagnosis of Mycobacterium bovis infection in cattle, but these tests detect only a portion of the infected animals. The aim of the present study was to evaluate the diagnostic coverage of a combination of the bTB antemortem techniques known as the comparative intradermal tuberculin test (CITT) and an ELISA based on a recombinant chimera of ESAT-6/MPB70/MPB83 as the antigen in cattle. The results were compared to postmortem findings based on M. bovis culturing and PCR. Paired comparisons of all data (n=92) demonstrated that ELISA and LST results compared to the culturing results did not present significant differences (P=0.27 on McNemar?s test and P=0.12 on Fisher?s exact test, respectively). Using culturing as the gold standard, the sensitivity and specificity of ELISA were 79.5% (95% CI: 64.5?89.2%) and 75.5% (95% CI: 62.4?85.1%), respectively, whereas LST demonstrated 100% sensitivity (95% CI: 91.03? 100%) and 92.5% specificity (95% CI: 82.1?97.0%). The ELISA results did not reveal significant differences in relation to the LST results (P>0.99 on Fisher?s exact test). Using the latter as the gold standard, the sensitivity and specificity of ELISA were 79.1% (95% CI: 64.8?88.6%) and 79.6% (95% CI: 66.4?88.5%), respectively. The use of ELISA with the recombinant chimera of ESAT-6/MPB70/ MPB83 as the antigen complements the diagnostic coverage provided by CITT and increases the removal of infected animals from herds.
Palavras-Chave: | Comparative intradermal tuberculin test.
Thesaurus Nal: | Bovine tuberculosis; Mycobacterium bovis BCG.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/207905/1/ELISA-using-a-recombinant-chimera.pdf
Registro original: | Embrapa Gado de Corte (CNPGC)

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