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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
02/03/2021 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CUNHA, A. F.; ANDRADE, H. M.; SOUZA, F. N.; FIALHO JUNIOR, L. C.; ROSA, D. L. S. O.; SANCHEZ, E. M. R.; GIDLUND, M.; GOTO, H.; BRITO, M. A. V. P.; GUIMARÃES, A. S.; LAGE, A. P.; REIS, L. C.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. |
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Afiliação: |
A. F. Cunha, UFMG; H. M. Andrade, UFMG; F. N. Souza, UFMG; L. C. Fialho Júnior, UFMG; D. L. S. O. Rosa, UFMG; E. M. Ramos Sanchez, USP; M. Gidlund, USP; H. Goto, USP; M. A. V. P. Brito, Pesquisadora aposentada da Embrapa Gado de Leite.; ALESSANDRO DE SA GUIMARAES, CNPGL; A. P. Lage, UFMG; L. C. Reis, USP; A. M. M. P. Della Libera, USP; M. B. Heinemann, USP; M. M. O. P. Cerqueira, UFMG. |
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Título: |
Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 103, n. 5, p. 4588-4605, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets. MenosABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among ani... Mostrar Tudo |
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Palavras-Chave: |
Immunoproteomics; Intramammary infection; Serological proteome analysis. |
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Thesagro: |
Staphylococcus Aureus. |
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Thesaurus Nal: |
Vaccines. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02811naa a2200349 a 4500
001 2130440
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008 2020 bl uuuu u00u1 u #d
100 1 $aCUNHA, A. F.
245 $aComparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine.$h[electronic resource]
260 $c2020
520 $aABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets.
650 $aVaccines
650 $aStaphylococcus Aureus
653 $aImmunoproteomics
653 $aIntramammary infection
653 $aSerological proteome analysis
700 1 $aANDRADE, H. M.
700 1 $aSOUZA, F. N.
700 1 $aFIALHO JUNIOR, L. C.
700 1 $aROSA, D. L. S. O.
700 1 $aSANCHEZ, E. M. R.
700 1 $aGIDLUND, M.
700 1 $aGOTO, H.
700 1 $aBRITO, M. A. V. P.
700 1 $aGUIMARÃES, A. S.
700 1 $aLAGE, A. P.
700 1 $aREIS, L. C.
700 1 $aLIBERA, A. M. M. P. D.
700 1 $aHEINEMANN, M. B.
700 1 $aCERQUEIRA, M. M. O. P
773 $tJournal of Dairy Science$gv. 103, n. 5, p. 4588-4605, 2020.
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Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 7 | |
| 2. |  | FOTIUS, A. B. M.; SILVA, M. N. P. da; ALVES, F. C.; RIBEIRO, P. R. de A.; VOLTOLINI, T. V.; FERNANDES JUNIOR, P. I. Respostas produtivas do capim-buffel inoculado com bactérias promotoras de crescimento. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 17., 2023, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2023. p. 36. (Embrapa Semiárido. Eventos Técnicos & Científicos, 1).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 3. | | FIGUEIRODO, L. B. F. DE; MORAES, S. A. de; RIBEIRO, L. G. M.; PINTO, A. F. de B. P.; SILVA, M. N. P. da; NOGUEIRA, D. M.; CORDEIRO, M. F.; LOPES JÚNIOR, E. S. Efeito de diferentes níveis de torta de algodão incluídos na dieta sobre a performance reprodutiva de cabras no semiárido nordestinO. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 55.; CONGRESSO BRASILEIRO DE ZOOTECNIA, 28., 2018, Goiânia, GO. Construindo saberes, formando pessoas e transformando a produção animal. Goiânia, GO: Sociedade Brasileira de Zootecnia: Associação Brasileira de Zootecnistas, 2018.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 4. | | GOMES, M. L. R.; ALVES, F. C.; SILVA FILHO, J. R. V. da; SOUZA, C. M. de; SILVA, M. N. P. da; SANTANA JUNIOR, R. A.; SOUZA, L. C. de; VOLTOLINI, T. V. Maniçoba for sheep and goats - forage yield, conservation strategies, animal performance and quality of products. Ciência Rural, v. 52, n. 3, e20201096, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Semiárido. |
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| 5. | | GOMES, M. L. R.; SILVA FILHO, J. R. V. da; ALVES, F. C.; SILVA, M. N. P. da; SOUZA, C. M. de; SOUZA, L. C. de; VOLTOLINI, T. V. In situ ruminal degradability of forage cactus-based diets associated with pornunça silage. Semina: Ciências Agrárias, v. 44, n. 2, p. 549-566, mar./abr. 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Semiárido. |
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| 6. | | RIBEIRO, L. G. M.; BASTOS, B. D. M.; SILVA, M. N. P.; OLIVEIRA, R. A.; PINTO, A. F. B. P.; LIMA, I. R. A.; MELO, L. H. A.; SOUSA, R. K. C.; GONÇALVES, P. R.; BONFA, H. C.; CORDEIRO, M. F.; NOGUEIRA, D. M.; BARBOSA, R. S.; LOPES JÚNIOR, E. S. Efeito da somatotropina bovina recombinante (rbST) na produção in vivo e na maturação in vitro de oócitos bovinos da raça Sindi. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.72, n. 1, p. 25-32, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Clima Temperado; Embrapa Semiárido. |
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| 7. | | SILVA, A. A. A.; SILVA, M. N. P.; FIGUEIREDO, L. B. F.; GONÇALVES, J. D.; SILVA, M. J. S.; LOIOLA, M. L. G.; BASTOS, B. D. M.; OLIVEIRA, R. A.; RIBEIRO, L. G. M.; BARBERINO, R. S.; GOUVEIA, B. B.; MONTE, A. P. O.; NOGUEIRA, D. M.; CORDEIRO, M. F.; MATOS, M. H. T.; LOPES JÚNIOR, E. S. Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes. Zygote, v. 26, n. 6, p. 465-470, dez. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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