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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
11/12/2020 |
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Data da última atualização: |
11/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARANTES, L. G.; TONELLI, G. S. S. S.; MARTINS, C. F.; BÁO, S. N. |
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Afiliação: |
CARLOS FREDERICO MARTINS, CPAC. |
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Título: |
Cellular Characterization and Effects Of Cryoprotectant Solutions on the Viability Of Fibroblasts from Three Brazilian Wild Cats. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Biopreservation and Biobanking, 2020. |
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Idioma: |
Português |
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Conteúdo: |
Preserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats' cells in cloning techniques, contributing directly to preserving wild fauna. MenosPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating... Mostrar Tudo |
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Palavras-Chave: |
Material genético; Protocolo. |
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Thesagro: |
Criopreservação. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02696naa a2200193 a 4500
001 2128006
005 2020-12-11
008 2020 bl uuuu u00u1 u #d
100 1 $aARANTES, L. G.
245 $aCellular Characterization and Effects Of Cryoprotectant Solutions on the Viability Of Fibroblasts from Three Brazilian Wild Cats.$h[electronic resource]
260 $c2020
520 $aPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats' cells in cloning techniques, contributing directly to preserving wild fauna.
650 $aCriopreservação
653 $aMaterial genético
653 $aProtocolo
700 1 $aTONELLI, G. S. S. S.
700 1 $aMARTINS, C. F.
700 1 $aBÁO, S. N.
773 $tBiopreservation and Biobanking, 2020.
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| Registros recuperados : 62 | |
| 6. |  | SILVA, L. R. da; CARVALHO, R. da S.; CARDOSO, R. M. C. B. Avaliação participativa da resistência de clones de mandioca (mesa e indústria) à mosca branca na Comunidade Cachoeira Grande, P. Tancredo Neves - BA. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro: [anais]. Cruz das Almas, BA, Embrapa Mandioca e Fruticultura, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | SARMENTO, J. D. A.; SILVA, L. R. da; ALVES, R. E.; MAIA, L. K. Composição químico-bromatológica de mandacaru (Cereus jamacaru P.) provenientes do vale do Curu-CE. In: CONGRESSO NACIONAL DE BOTÂNICA, 59.; REUNIÃO NORDESTINA DE BOTÂNICA, 31.; CONGRESSO LATINO AMERICANO Y DEL CARIBE DE CACTÁCEAS Y OTRAS SUCULENTAS, 4.; CONGRESS OF INTERNATIONAL ORGANIZATION FOR SUCULENT PLANT STUDY, 30., 2008, Natal. Atualidades, desafios e perspectivas da botânica no Brasil: anais. Natal: UFERSA: UFRN: SBB, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 12. | | PELAEZ, V.; TEODOROVICZ, T.; GUIMARÃES, T. A.; SILVA, L. R. da; MOREAU, D.; MIZUKAWA, G. A dinâmica do comércio internacional de agrotóxicos. Revista de Política Agrícola, Brasília, DF, ano 25, n. 2, p. 39-52, abr./maio/jun. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 17. | | SILVA, L. R. da; FIGUEIREDO, A. R. de; BARBOSA, M. I. M. J.; VILLA, F.; MORAIS, L. A. S. de. Avaliação físico-química e compostos bioativos de frutos da groselha do Ceilão (Dovyalis hebecarpa Warb). In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 22., 2022, Seropédica. Bicentenário da Independência: 200 anos de ciência, tecnologia e inovação no Brasil: caderno de resumos... Seropédica: Embrapa Agrobiologia, 2022.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 19. | | NABESHIMA, E. H.; MIRANDA, M. Z. de; SILVA, L. R. da; REYES, R. S.; CLERICI, M. T. P. S. Qualidade sanitária de cereais e pseudocereais: comparativo entre farinha obtida com e sem reconstituição. Research, Society and Development, v. 13, n. 12, e80131247686, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Trigo. |
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| 20. | | FIGUEIREDO, A. R. de; SILVA, L. R. da; CAMARGO, S. M. B.; MORAIS, L. A. S. de. Morphological and phytochemical characterization of Lavandula dentata L. cultivated in Paraíba do Sul, Rio de Janeiro, Brazil. Acta Brasiliensis, v. 5, n. 1, p. 7-12, 2021. Caracterização morfológica e fitoquímica de Lavandula dentata L. cultivada na Paraíba do Sul, Rio de Janeiro, Brasil.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 62 | |
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