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81. | | SILVA, K. da; ZILLI, J. E.; PERIN, L.; GOMES, M. de L.; BARAÚNA, A. C.; PEREIRA, G. M. D. Caracterização genotípica de bactérias diazotróficas endofíticas isoladas de milho cultivado em Roraima. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia, MG. Anais... Uberlândia, MG: 2011. Biblioteca(s): Embrapa Roraima. |
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82. | | COSTA, I. B. da; BARAÚNA, A. C.; FARIAS, E. do N. C.; ZILLI, J. E.; SILVA, K. da. Caracterização genotípica de bactérias isoladas de pau-rainha. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 34., 2013, Florianópolis. Ciência do solo: para quê e para quem: anais. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2013. Biblioteca(s): Embrapa Roraima. |
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84. | | SILVA, K. da; CHAVES, J. da S.; BARAÚNA, A. C.; MOSQUEIRA, C. A.; GIANLUPPI, V.; ZILLI, J. E. Caracterização fenotípica de bactérias fixadoras de nitrogênio isoladas de estilosantes (styloshantes spp.) Isoladas no Cerrado de Roraima. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 34., 2013, Florianópolis. Ciência do solo: para quê e para quem: anais. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2013. Biblioteca(s): Embrapa Roraima. |
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87. | | SMIDERLE, O. J.; SILVA NETO, M. L. da; ZILLI, J. E.; SILVA, K. da; SOUZA, A. das G. Controlled management of seed and good performance on germination and seedling initial growth of cowpea. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF : Embrapa, 2016. Biblioteca(s): Embrapa Roraima. |
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88. | | ZILLI, J. E.; PEREIRA, G. M. D.; FRANÇA JÚNIOR, I.; SILVA, K. da; HUNGRIA, M.; ROUWS, J. R. C. Dinâmica de rizóbios em solo do cerrado de Roraima durante o período de estiagem. Acta Amazonica, Manaus v. 43, n. 2, p. 153-160, 2013. Biblioteca(s): Embrapa Agrobiologia; Embrapa Roraima; Embrapa Soja. |
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89. | | PEPE, K. B. F.; TULIO, R. H.; SILVA, K. da; ZANATTA, J. A.; RACHWAL, M. F. G. Density of ammonificators and nitrifiers bacteria in soils cultiv ated with pine and native forest in the South of Brazil. Pesquisa Florestal Brasileira, Colombo, v. 39, (nesp), e201902043, 2019. p. 671. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba. Biblioteca(s): Embrapa Florestas. |
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90. | | SILVA, K. da; NOBREGA, R. S. A.; LIMA, A. S.; BARBERI, A.; MOREIRA, F. M. de S. Density and diversity of diazotrophic bacteria isolated from Amazonian soils using N-free semi-solid media. Sci. Agric., v.68, n.5, p.518-525, Sep./Oct., 2011 Biblioteca(s): Embrapa Roraima. |
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92. | | BARAÚNA, A. C.; SILVA, K. da; PEREIRA, G. M. D.; KAMINSKI, P. E.; PERIN, L.; ZILLI, J. E. Diversity and nitrogen fixation efficiency of rhizobia isolated from nodules of Centrolobium paraense. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 4, p. 296-305, abr. 2014. Título em português: Diversidade e eficiência na fixação do nitrogênio de rizóbios isolados de nódulos de Centrolobium paraense. Biblioteca(s): Embrapa Agrobiologia; Embrapa Roraima; Embrapa Unidades Centrais. |
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96. | | MELO, A. M. R. de; BARAUNA, A. C.; BRECKENFELD, I.; PEREIRA, G. M. D.; SILVA, K. da; ZILLI, J. E. Rizóbios associados às raízes de Pau-rainha em solos de Roraima REUNIÃO BRASILEIRA DE CIÊNCIA DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa, MG: SBCS, 2012. FERTBIO 2012. 4 p. CD-ROM. Resumo expandido. Biblioteca(s): Embrapa Agrobiologia. |
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97. | | MELO, A. M. R. de; BARAUNA, A. C.; BRECKENFELD, I.; PEREIRA, G. M. D.; SILVA, K. da; ZILLI, J. E. Rizóbios associados às raízes de Pau-rainha em solos de Roraima REUNIÃO BRASILEIRA DE CIÊNCIA DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa, MG: SBCS, 2012. FERTBIO 2012. 4 p. CD-ROM. Resumo expandido. Biblioteca(s): Embrapa Roraima. |
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100. | | COSTA, I. B. da; CHALITA, P. B.; FARIAS, E. do N. C.; ALBUQUERQUE, T. C. S. de; SILVA, K. da. Ocorrência e isolamento de bactérias diazotróficas Associativas de raízes de Castanha-do-Brasil. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 31.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 15.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 13.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 10., 2014, Araxá. Fertilidade e biologia do solo: integração e tecnologias para todos: anais. Araxá: Núcleo Regional Leste da Sociedade Brasileira de Ciência do Solo, 2014. FertBio 2014. Biblioteca(s): Embrapa Roraima. |
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Registros recuperados : 157 | |
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Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
15/08/2016 |
Data da última atualização: |
02/12/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
CHAVES, J. da S.; BARAÚNA, A. C.; MOSQUEIRA, C. A.; GIANLUPPI, V.; ZILLI, J. E.; SILVA, K. da. |
Afiliação: |
JOSIMAR DA SILVA CHAVES, UFRR; ALEXANDRE CARDOSO BARAÚNA, UFRRJ; CÁTIA APARECIDA MOSQUEIRA, UFRR; VICENTE GIANLUPPI, CPAF-Roraima; JERRI EDSON ZILLI, CNPAB; KRISLE DA SILVA, CPAF-Roraima. |
Título: |
Stylosanthes spp. from Amazon savanna harbour diverse and potentially effective rhizobia. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Applied Soil Ecology, v. 108, p. 54-61, 2016 |
Idioma: |
Inglês |
Conteúdo: |
The Stylosanthes genus stands out among the forage legume species well-adapted to the soil and climatic conditions of tropical regions. This legume establishes a symbiotic relationship with nitrogen-fixing bacteria, which can be used to improve the quality of pastures. However, there are few studies about the diversity and symbiotic efficiency of nitrogen-fixing bacteria in Stylosanthes plants. Therefore, the objective was to isolate, characterize and evaluate the symbiotic efficiency of nitrogen-fixing bacteria in symbiosis with Stylosanthes in savanna areas in Roraima, Brazil. Plants with nodules were collected at 6 sites, including cultivated and native areas of the savanna. Shoots of Stylosanthes with flowers were collected to prepare exsiccatae for species identification. The nodules were surface-sterilized with 5% sodium hypochlorite after the roots were washed, and isolation and morphological characterization were performed in 79 culture medium. The isolates were selected according to their morphology, the representativeness of each collection site and the associated Stylosanthes species. Representative isolates were tested for nodulation in cowpea plants, and the isolates able to nodulate the gene 16S rRNA was sequenced. The symbiotic efficiency was evaluated in Stylosanthes capitata cv. Lavradeiro in greenhouse conditions for 65 days. A total of 504 nodules were collected, from which 258 pure isolates were obtained from 5 species of Stylosanthes (S. capitata,S. scabra, S. gracilis, S. humilis and S. angustifolia). The isolates showed significant phenotypic diversity, and 89 isolates were selected. Thirty-one isolates were confirmed to be nodulating bacteria in cowpea plants. These isolates were identified as a-Proteobacteria, with Bradyrhizobium, rhizobium and Mesorhizobium genera, and b-Proteobacteria, with Burkholderia and Herbaspirillum genera. When evaluated in S. capitata, treatment with two Bradyrhizobium isolates, ERR 1178 and ERR 942, resulted in significant production of plant biomass production and N accumulation. These results indicated that a large diversity of a-Proteobacteria and b-Proteobacteria are able to nodulate Stylosanthes spp. and some isolates have potential for testing under
field conditions. MenosThe Stylosanthes genus stands out among the forage legume species well-adapted to the soil and climatic conditions of tropical regions. This legume establishes a symbiotic relationship with nitrogen-fixing bacteria, which can be used to improve the quality of pastures. However, there are few studies about the diversity and symbiotic efficiency of nitrogen-fixing bacteria in Stylosanthes plants. Therefore, the objective was to isolate, characterize and evaluate the symbiotic efficiency of nitrogen-fixing bacteria in symbiosis with Stylosanthes in savanna areas in Roraima, Brazil. Plants with nodules were collected at 6 sites, including cultivated and native areas of the savanna. Shoots of Stylosanthes with flowers were collected to prepare exsiccatae for species identification. The nodules were surface-sterilized with 5% sodium hypochlorite after the roots were washed, and isolation and morphological characterization were performed in 79 culture medium. The isolates were selected according to their morphology, the representativeness of each collection site and the associated Stylosanthes species. Representative isolates were tested for nodulation in cowpea plants, and the isolates able to nodulate the gene 16S rRNA was sequenced. The symbiotic efficiency was evaluated in Stylosanthes capitata cv. Lavradeiro in greenhouse conditions for 65 days. A total of 504 nodules were collected, from which 258 pure isolates were obtained from 5 species of Stylosanthes (S. capitata,S. scab... Mostrar Tudo |
Palavras-Chave: |
16S rRNA; Biological nitrogen fixation; Eficiência simbiótica; FBN; Forragem tropical; Symbiotic efficiency; Tropical forage. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 03033naa a2200265 a 4500 001 2050888 005 2016-12-02 008 2016 bl --- 0-- u #d 100 1 $aCHAVES, J. da S. 245 $aStylosanthes spp. from Amazon savanna harbour diverse and potentially effective rhizobia.$h[electronic resource] 260 $c2016 520 $aThe Stylosanthes genus stands out among the forage legume species well-adapted to the soil and climatic conditions of tropical regions. This legume establishes a symbiotic relationship with nitrogen-fixing bacteria, which can be used to improve the quality of pastures. However, there are few studies about the diversity and symbiotic efficiency of nitrogen-fixing bacteria in Stylosanthes plants. Therefore, the objective was to isolate, characterize and evaluate the symbiotic efficiency of nitrogen-fixing bacteria in symbiosis with Stylosanthes in savanna areas in Roraima, Brazil. Plants with nodules were collected at 6 sites, including cultivated and native areas of the savanna. Shoots of Stylosanthes with flowers were collected to prepare exsiccatae for species identification. The nodules were surface-sterilized with 5% sodium hypochlorite after the roots were washed, and isolation and morphological characterization were performed in 79 culture medium. The isolates were selected according to their morphology, the representativeness of each collection site and the associated Stylosanthes species. Representative isolates were tested for nodulation in cowpea plants, and the isolates able to nodulate the gene 16S rRNA was sequenced. The symbiotic efficiency was evaluated in Stylosanthes capitata cv. Lavradeiro in greenhouse conditions for 65 days. A total of 504 nodules were collected, from which 258 pure isolates were obtained from 5 species of Stylosanthes (S. capitata,S. scabra, S. gracilis, S. humilis and S. angustifolia). The isolates showed significant phenotypic diversity, and 89 isolates were selected. Thirty-one isolates were confirmed to be nodulating bacteria in cowpea plants. These isolates were identified as a-Proteobacteria, with Bradyrhizobium, rhizobium and Mesorhizobium genera, and b-Proteobacteria, with Burkholderia and Herbaspirillum genera. When evaluated in S. capitata, treatment with two Bradyrhizobium isolates, ERR 1178 and ERR 942, resulted in significant production of plant biomass production and N accumulation. These results indicated that a large diversity of a-Proteobacteria and b-Proteobacteria are able to nodulate Stylosanthes spp. and some isolates have potential for testing under field conditions. 653 $a16S rRNA 653 $aBiological nitrogen fixation 653 $aEficiência simbiótica 653 $aFBN 653 $aForragem tropical 653 $aSymbiotic efficiency 653 $aTropical forage 700 1 $aBARAÚNA, A. C. 700 1 $aMOSQUEIRA, C. A. 700 1 $aGIANLUPPI, V. 700 1 $aZILLI, J. E. 700 1 $aSILVA, K. da 773 $tApplied Soil Ecology$gv. 108, p. 54-61, 2016
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