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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
19/02/2019 |
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Data da última atualização: |
19/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
POLETI, M. D.; REGITANO, L. C. de A.; SOUSA, G. H. M. F.; CESAR, A. S. M.; SIMAS, R. C.; SILVA-VIGNATO, B.; OLIVEIRA, G. B.; ANDRADE, S. C. S.; CAMERON, L. C.; COUTINHO, L. L. |
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Afiliação: |
Mirele D. Poleti, USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; Gustavo H. M. F. Souza, MS Applications and Development Laboratory Waters Corporation; Aline S. M. Cesar, USP; Rosineide C. Simas, USP; Bárbara Silva-Vignato, USP; Gabriella B. Oliveira, USP; Sónia C. S. Andrade, USP; Luiz C. Cameron, UNIRIO; Luiz Lehmann Coutinho, USP. |
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Título: |
Longissimus dorsi muscle label-free quantitative proteomic reveals biological mechanisms associated with intramuscular fat deposition. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Journal of Proteomics, v. 179, p. 30-41, 2018. |
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DOI: |
https://doi.org/10.1016/j.dib.2018.06.004 |
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Idioma: |
Inglês |
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Conteúdo: |
The pathways involved in intramuscular fat (IMF) deposition in Longissimus dorsi muscle were investigated using an integrated transcriptome-assisted label-free quantitative proteomic approach by High Definition Mass Spectrometry. We quantified 1582 proteins, of which 164 were differentially abundant proteins (DAPs, p < 0.05) between animals with high (H) and low (L) genomic estimated breeding values (GEBV) for IMF content. Ingenuity pathway analysis (IPA) revealed that these DAPs were mainly involved in glycolysis metabolism, actin cytoskeleton signaling, cell-cell adherens junction and pathways for MAPK and insulin. A comparative study between transcriptomic (mRNA) and proteomic data showed 17 differentially expressed genes corresponding to DAPs, of which three genes/proteins did not agree on the direction of the fold change between groups. Moreover, we investigated microRNAs data to explain these differences in fold change direction, being able to unravel two of the three unexpected mRNA/protein relationships. Results demonstrated that changes in protein/mRNA levels of sarcomere organization, intracellular signal transduction and regulation of actin cytoskeleton, are involved in IMF deposition. These findings provide a deeper understanding of the highly complex regulatory mechanisms involved in IMF deposition in cattle and indicate target pathways for future studies. Significance: Intramuscular fat is the amount of fat deposited inside muscle and plays an important role in human health and meat quality attributes, influencing energy metabolism of skeletal muscle, as well as, tenderness, flavor, and juiciness of beef. We performed for the first time the utilization of integrated transcriptome-assisted label-free quantitative proteomic approach using High Definition Mass Spectrometry for characterization of the changes in the proteomic profile of the Longissimus dorsi muscle associated with intramuscular fat deposition in cattle. Furthermore, we compared the muscle proteome with the muscle transcriptome (mRNA and microRNAs), obtained by RNA-sequencing, to better understand the relationship between expression of mRNAs and proteins and to unravel essential biological mechanisms involved in bovine skeletal muscle IMF deposition. MenosThe pathways involved in intramuscular fat (IMF) deposition in Longissimus dorsi muscle were investigated using an integrated transcriptome-assisted label-free quantitative proteomic approach by High Definition Mass Spectrometry. We quantified 1582 proteins, of which 164 were differentially abundant proteins (DAPs, p < 0.05) between animals with high (H) and low (L) genomic estimated breeding values (GEBV) for IMF content. Ingenuity pathway analysis (IPA) revealed that these DAPs were mainly involved in glycolysis metabolism, actin cytoskeleton signaling, cell-cell adherens junction and pathways for MAPK and insulin. A comparative study between transcriptomic (mRNA) and proteomic data showed 17 differentially expressed genes corresponding to DAPs, of which three genes/proteins did not agree on the direction of the fold change between groups. Moreover, we investigated microRNAs data to explain these differences in fold change direction, being able to unravel two of the three unexpected mRNA/protein relationships. Results demonstrated that changes in protein/mRNA levels of sarcomere organization, intracellular signal transduction and regulation of actin cytoskeleton, are involved in IMF deposition. These findings provide a deeper understanding of the highly complex regulatory mechanisms involved in IMF deposition in cattle and indicate target pathways for future studies. Significance: Intramuscular fat is the amount of fat deposited inside muscle and plays an important role in... Mostrar Tudo |
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Palavras-Chave: |
Adiposidade; Gordura intramuscular; MRNA. |
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Thesagro: |
Gordura Animal. |
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Thesaurus Nal: |
Mass spectrometry; MicroRNA. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03231naa a2200313 a 4500
001 2106242
005 2019-02-19
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.dib.2018.06.004$2DOI
100 1 $aPOLETI, M. D.
245 $aLongissimus dorsi muscle label-free quantitative proteomic reveals biological mechanisms associated with intramuscular fat deposition.$h[electronic resource]
260 $c2018
520 $aThe pathways involved in intramuscular fat (IMF) deposition in Longissimus dorsi muscle were investigated using an integrated transcriptome-assisted label-free quantitative proteomic approach by High Definition Mass Spectrometry. We quantified 1582 proteins, of which 164 were differentially abundant proteins (DAPs, p < 0.05) between animals with high (H) and low (L) genomic estimated breeding values (GEBV) for IMF content. Ingenuity pathway analysis (IPA) revealed that these DAPs were mainly involved in glycolysis metabolism, actin cytoskeleton signaling, cell-cell adherens junction and pathways for MAPK and insulin. A comparative study between transcriptomic (mRNA) and proteomic data showed 17 differentially expressed genes corresponding to DAPs, of which three genes/proteins did not agree on the direction of the fold change between groups. Moreover, we investigated microRNAs data to explain these differences in fold change direction, being able to unravel two of the three unexpected mRNA/protein relationships. Results demonstrated that changes in protein/mRNA levels of sarcomere organization, intracellular signal transduction and regulation of actin cytoskeleton, are involved in IMF deposition. These findings provide a deeper understanding of the highly complex regulatory mechanisms involved in IMF deposition in cattle and indicate target pathways for future studies. Significance: Intramuscular fat is the amount of fat deposited inside muscle and plays an important role in human health and meat quality attributes, influencing energy metabolism of skeletal muscle, as well as, tenderness, flavor, and juiciness of beef. We performed for the first time the utilization of integrated transcriptome-assisted label-free quantitative proteomic approach using High Definition Mass Spectrometry for characterization of the changes in the proteomic profile of the Longissimus dorsi muscle associated with intramuscular fat deposition in cattle. Furthermore, we compared the muscle proteome with the muscle transcriptome (mRNA and microRNAs), obtained by RNA-sequencing, to better understand the relationship between expression of mRNAs and proteins and to unravel essential biological mechanisms involved in bovine skeletal muscle IMF deposition.
650 $aMass spectrometry
650 $aMicroRNA
650 $aGordura Animal
653 $aAdiposidade
653 $aGordura intramuscular
653 $aMRNA
700 1 $aREGITANO, L. C. de A.
700 1 $aSOUSA, G. H. M. F.
700 1 $aCESAR, A. S. M.
700 1 $aSIMAS, R. C.
700 1 $aSILVA-VIGNATO, B.
700 1 $aOLIVEIRA, G. B.
700 1 $aANDRADE, S. C. S.
700 1 $aCAMERON, L. C.
700 1 $aCOUTINHO, L. L.
773 $tJournal of Proteomics$gv. 179, p. 30-41, 2018.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
24/01/2023 |
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Data da última atualização: |
27/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
GOMES, V. H. F.; SIMOES, W. L.; SILVA, J. S. da; GARRIDO, M. da S.; SILVA, J. A. B. da; LOPES, P. R. C.; SILVA, W. O. da; SANTOS, L. R. dos. |
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Afiliação: |
VICTOR H. F. GOMES, Universidade Brasília Educacional, Juazeiro, BA; WELSON LIMA SIMOES, CPATSA; JUCICLÉIA S. DA SILVA; MARLON DA S. GARRIDO, UNIVASF; JOSÉ A. B. DA SILVA, UNIVASF; PAULO ROBERTO COELHO LOPES, CPATSA; WESLLEY O. DA SILVA, UPE; LEANE R. DOS SANTOS, Universidade do Estado da Bahia, Euclides da Cunha, BA. |
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Título: |
Production, gas and biochemical exchanges in pear cultivated in semi-arid region under different irrigation managements. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Revista Brasileira de Engenharia Agrícola e Ambiental, v. 27, n. 5, p. 335-342, 2023. |
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ISSN: |
1807-1929 |
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DOI: |
http://dx.doi.org/10.1590/1807-1929/agriambi.v27n5p335-342 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to evaluate the influence of irrigation systems and water depths on physiological, biochemical and production processes of pear trees grown in the Brazilian semi-arid region. The experimental design was randomized blocks, with 2 × 4 factorial scheme, corresponding to two irrigation systems (drip and microsprinkler) and four irrigation depths (60, 80, 100 and 120% of the crop evapotranspiration - ETc), with four replicates. Water deficit and excess applied to plants are harmful to gas exchange as well as to biosynthesis and accumulation of carbohydrates, amino acids and proteins in leaves, compromising the cultivation cycle of pear plants grown in the Sub-Middle São Francisco region. The irrigation depth of 91.8% ETc promotes the highest production of pear (18.49 kg plant -1) under drip and microsprinkler irrigation systems, under the edaphoclimatic conditions of the Sub-Middle São Francisco region. |
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Palavras-Chave: |
Lâminas de irrigação; Manejo de irrigação; Semiárido. |
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Thesagro: |
Irrigação; Irrigação Localizada; Pêra; Pyrus Communis. |
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Thesaurus NAL: |
Irrigation; Plant Science and Plant Products. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1151183/1/Production-gas-and-biochemical-exchanges-in-pear-cultivated-Pera-Irrigacao-2023.pdf
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Marc: |
LEADER 02037naa a2200337 a 4500
001 2151183
005 2023-03-27
008 2023 bl uuuu u00u1 u #d
022 $a1807-1929
024 7 $ahttp://dx.doi.org/10.1590/1807-1929/agriambi.v27n5p335-342$2DOI
100 1 $aGOMES, V. H. F.
245 $aProduction, gas and biochemical exchanges in pear cultivated in semi-arid region under different irrigation managements.$h[electronic resource]
260 $c2023
520 $aThe objective of this study was to evaluate the influence of irrigation systems and water depths on physiological, biochemical and production processes of pear trees grown in the Brazilian semi-arid region. The experimental design was randomized blocks, with 2 × 4 factorial scheme, corresponding to two irrigation systems (drip and microsprinkler) and four irrigation depths (60, 80, 100 and 120% of the crop evapotranspiration - ETc), with four replicates. Water deficit and excess applied to plants are harmful to gas exchange as well as to biosynthesis and accumulation of carbohydrates, amino acids and proteins in leaves, compromising the cultivation cycle of pear plants grown in the Sub-Middle São Francisco region. The irrigation depth of 91.8% ETc promotes the highest production of pear (18.49 kg plant -1) under drip and microsprinkler irrigation systems, under the edaphoclimatic conditions of the Sub-Middle São Francisco region.
650 $aIrrigation
650 $aPlant Science and Plant Products
650 $aIrrigação
650 $aIrrigação Localizada
650 $aPêra
650 $aPyrus Communis
653 $aLâminas de irrigação
653 $aManejo de irrigação
653 $aSemiárido
700 1 $aSIMOES, W. L.
700 1 $aSILVA, J. S. da
700 1 $aGARRIDO, M. da S.
700 1 $aSILVA, J. A. B. da
700 1 $aLOPES, P. R. C.
700 1 $aSILVA, W. O. da
700 1 $aSANTOS, L. R. dos
773 $tRevista Brasileira de Engenharia Agrícola e Ambiental$gv. 27, n. 5, p. 335-342, 2023.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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