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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
07/02/2024 |
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Data da última atualização: |
07/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SILVA, H. A. da; PESSÔA, D. D. V.; MENESES, C. H. G. de; CAETANTO, V. S.; PACHECO, R. S.; ARAUJO, J. L. S. de. |
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Afiliação: |
HELDER ANDERSON DA SILVA, UFRJ; DANIELLA DUARTE VILLARINHO PESSÔA, UFRJ; CARLOS HENRIQUE GADELHA DE MENESES, UFPB; VANESSA SANTANA CAETANO, UFRJ; RAFAEL SANCHES PACHECO, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB. |
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Título: |
Unraveling the drought-responsive transcriptomes in nodules of two common bean genotypes during biological nitrogen fixation. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 15, 134539, jan. 2024. Sec. Plant Symbiotic Interactions |
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ISSN: |
1664-462X |
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DOI: |
https://doi.org/10.3389/fpls.2024.1345379 |
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Idioma: |
Inglês |
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Conteúdo: |
ommon bean (Phaseolus vulgaris) can efficiently fix atmospheric nitrogen when associated with Rhizobia. However, drought stress impairs plant metabolic processes, especially the biological nitrogen fixation (BNF). Here, we assessed transcriptional responses in nodules of two common bean genotypes to drought stress under BNF reliance. The RNA-Seq analysis yielded a total of 81,489,262 and 72,497,478 high quality reads for Negro Argel and BAT 477 genotypes, respectively. The reads were mapped to the Phaseolus vulgaris reference genome and expression analysis identified 145 and 1451 differentially expressed genes (DEGs) for Negro Argel and BAT 477 genotypes, respectively. Although BAT 477 had more DEGs, both genotypes shared certain drought-responsive genes, including an up-regulated heat shock protein (HSP) and a down-regulated peroxidase, indicating shared pathways activated during drought in nodule tissue. Functional analysis using MapMan software highlighted the up-regulation of genes involved in abiotic stress responses, such as HSPs and specific transcription factors (TFs), in both genotypes. There was a significant down-regulation in metabolic pathways related to antioxidant protection, hormone signaling, metabolism, and transcriptional regulation. To validate these findings, we conducted RT-qPCR experiments for ten DEGs in nodules from both genotypes, for which the expression profile was confirmed, thus reinforcing their functional relevance in the nodule responses to drought stress during BNF. BAT 477 genotype exhibited more pronounced response to drought, characterized by a high number of DEGs. The strong down-regulation of DEGs leads to transcriptional disturbances in several pathways related to stress acclimation such as hormone and antioxidant metabolism. Additionally, we identified several genes that are known to play key roles in enhancing drought tolerance, such as HSPs and crucial TFs. Our results provide new insights into the transcriptional responses in root-nodules, an underexplored tissue of plants mainly under drought conditions. This research paves the way for potential improvements in plant-bacteria interactions, contributing to common bean adaptations in the face of challenging environmental conditions. Menosommon bean (Phaseolus vulgaris) can efficiently fix atmospheric nitrogen when associated with Rhizobia. However, drought stress impairs plant metabolic processes, especially the biological nitrogen fixation (BNF). Here, we assessed transcriptional responses in nodules of two common bean genotypes to drought stress under BNF reliance. The RNA-Seq analysis yielded a total of 81,489,262 and 72,497,478 high quality reads for Negro Argel and BAT 477 genotypes, respectively. The reads were mapped to the Phaseolus vulgaris reference genome and expression analysis identified 145 and 1451 differentially expressed genes (DEGs) for Negro Argel and BAT 477 genotypes, respectively. Although BAT 477 had more DEGs, both genotypes shared certain drought-responsive genes, including an up-regulated heat shock protein (HSP) and a down-regulated peroxidase, indicating shared pathways activated during drought in nodule tissue. Functional analysis using MapMan software highlighted the up-regulation of genes involved in abiotic stress responses, such as HSPs and specific transcription factors (TFs), in both genotypes. There was a significant down-regulation in metabolic pathways related to antioxidant protection, hormone signaling, metabolism, and transcriptional regulation. To validate these findings, we conducted RT-qPCR experiments for ten DEGs in nodules from both genotypes, for which the expression profile was confirmed, thus reinforcing their functional relevance in the nodule responses to d... Mostrar Tudo |
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Palavras-Chave: |
Nodule; Plant-Bacteria Association; Transcriptome analysis. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 03089naa a2200241 a 4500
001 2161757
005 2024-02-07
008 2024 bl uuuu u00u1 u #d
022 $a1664-462X
024 7 $ahttps://doi.org/10.3389/fpls.2024.1345379$2DOI
100 1 $aSILVA, H. A. da
245 $aUnraveling the drought-responsive transcriptomes in nodules of two common bean genotypes during biological nitrogen fixation.$h[electronic resource]
260 $c2024
520 $aommon bean (Phaseolus vulgaris) can efficiently fix atmospheric nitrogen when associated with Rhizobia. However, drought stress impairs plant metabolic processes, especially the biological nitrogen fixation (BNF). Here, we assessed transcriptional responses in nodules of two common bean genotypes to drought stress under BNF reliance. The RNA-Seq analysis yielded a total of 81,489,262 and 72,497,478 high quality reads for Negro Argel and BAT 477 genotypes, respectively. The reads were mapped to the Phaseolus vulgaris reference genome and expression analysis identified 145 and 1451 differentially expressed genes (DEGs) for Negro Argel and BAT 477 genotypes, respectively. Although BAT 477 had more DEGs, both genotypes shared certain drought-responsive genes, including an up-regulated heat shock protein (HSP) and a down-regulated peroxidase, indicating shared pathways activated during drought in nodule tissue. Functional analysis using MapMan software highlighted the up-regulation of genes involved in abiotic stress responses, such as HSPs and specific transcription factors (TFs), in both genotypes. There was a significant down-regulation in metabolic pathways related to antioxidant protection, hormone signaling, metabolism, and transcriptional regulation. To validate these findings, we conducted RT-qPCR experiments for ten DEGs in nodules from both genotypes, for which the expression profile was confirmed, thus reinforcing their functional relevance in the nodule responses to drought stress during BNF. BAT 477 genotype exhibited more pronounced response to drought, characterized by a high number of DEGs. The strong down-regulation of DEGs leads to transcriptional disturbances in several pathways related to stress acclimation such as hormone and antioxidant metabolism. Additionally, we identified several genes that are known to play key roles in enhancing drought tolerance, such as HSPs and crucial TFs. Our results provide new insights into the transcriptional responses in root-nodules, an underexplored tissue of plants mainly under drought conditions. This research paves the way for potential improvements in plant-bacteria interactions, contributing to common bean adaptations in the face of challenging environmental conditions.
653 $aNodule
653 $aPlant-Bacteria Association
653 $aTranscriptome analysis
700 1 $aPESSÔA, D. D. V.
700 1 $aMENESES, C. H. G. de
700 1 $aCAETANTO, V. S.
700 1 $aPACHECO, R. S.
700 1 $aARAUJO, J. L. S. de
773 $tFrontiers in Plant Science$gv. 15, 134539, jan. 2024. Sec. Plant Symbiotic Interactions
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