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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
18/06/2019 |
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Data da última atualização: |
02/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VOLK, G. M.; JENDEREK, M. M.; STAATS, E.; SHEPHERD, A.; BONNART, R.; LEDO, A. da S.; AYALA SILVA. T. |
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Afiliação: |
ANA DA SILVA LEDO, CPATC. |
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Título: |
Challenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Acta Hortciulturae, 1234, 2019. |
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ISSN: |
2406-6168 |
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Idioma: |
Português |
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Notas: |
Apresentado no Acta Horticulturae, 1234. DOI 10.17660/ActaHor. |
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Conteúdo: |
The USDA-ARS National Plant Germplasm System (NPGS) maintains 946 accessions of sugarcane (Saccharum spp.) in the field at the Subtropical Horticult sure Research Station in Miami, Florida. These accessions are particularly vulnerable to hurricanes, diseases and other threats. We sought to identify a method whereby clonally propagated sugarcane accessions could be successfully introduced into tissue culture, multiplied, and then cryopreserved as shoot tips for long-term preservation at he National Laboratory for Genetic Resources Preservation in For Collins, Colorado. For many accessions, 70% isopropyl alcohol and 20% commercial bleach treatments, followed by three rinses of sterile water weresufficient to remove microbial contaminants during the introduction process. However, in some cases, cefotaxime was particularly effective for removing bacterial contamination. We found that antioxidant treatments of glutathione, glycine betaine, and ascorbic acid did not improve regrowth after liquid nitrogen exposure usine either PVS2 or PVS3 as cryoprotectants in droplet vitrification cryopreservation methods. Exposure durations of PVS2 and PVS3 were optimized, with and without exposure to liquid nitrogen (LN), and shoot tip regrowth levels ranged from 0 to 37% after LN exposure. Published methods for encapsulation dehydration and V-plate vitrification cryopreservation procedures were tested to determine if acceptable results could be obtained. Using these methods, shoot tip regrowth levels ranged from 0 to 50% after LN exposure. We conclude that the sugarcane cryopreservation methods that we tested are not yet ready for implementation in the NPGS. MenosThe USDA-ARS National Plant Germplasm System (NPGS) maintains 946 accessions of sugarcane (Saccharum spp.) in the field at the Subtropical Horticult sure Research Station in Miami, Florida. These accessions are particularly vulnerable to hurricanes, diseases and other threats. We sought to identify a method whereby clonally propagated sugarcane accessions could be successfully introduced into tissue culture, multiplied, and then cryopreserved as shoot tips for long-term preservation at he National Laboratory for Genetic Resources Preservation in For Collins, Colorado. For many accessions, 70% isopropyl alcohol and 20% commercial bleach treatments, followed by three rinses of sterile water weresufficient to remove microbial contaminants during the introduction process. However, in some cases, cefotaxime was particularly effective for removing bacterial contamination. We found that antioxidant treatments of glutathione, glycine betaine, and ascorbic acid did not improve regrowth after liquid nitrogen exposure usine either PVS2 or PVS3 as cryoprotectants in droplet vitrification cryopreservation methods. Exposure durations of PVS2 and PVS3 were optimized, with and without exposure to liquid nitrogen (LN), and shoot tip regrowth levels ranged from 0 to 37% after LN exposure. Published methods for encapsulation dehydration and V-plate vitrification cryopreservation procedures were tested to determine if acceptable results could be obtained. Using these methods, shoot tip regrowth... Mostrar Tudo |
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Thesagro: |
Cana de Açúcar. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198664/1/SugarcaneISHS2019.pdf
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Marc: |
LEADER 02355naa a2200229 a 4500
001 2109915
005 2019-10-02
008 2019 bl uuuu u00u1 u #d
022 $a2406-6168
100 1 $aVOLK, G. M.
245 $aChallenges in the development of a widely applicable method for sugarcane (Saccharum spp.) shoot tip cryopreservation.
260 $c2019
500 $aApresentado no Acta Horticulturae, 1234. DOI 10.17660/ActaHor.
520 $aThe USDA-ARS National Plant Germplasm System (NPGS) maintains 946 accessions of sugarcane (Saccharum spp.) in the field at the Subtropical Horticult sure Research Station in Miami, Florida. These accessions are particularly vulnerable to hurricanes, diseases and other threats. We sought to identify a method whereby clonally propagated sugarcane accessions could be successfully introduced into tissue culture, multiplied, and then cryopreserved as shoot tips for long-term preservation at he National Laboratory for Genetic Resources Preservation in For Collins, Colorado. For many accessions, 70% isopropyl alcohol and 20% commercial bleach treatments, followed by three rinses of sterile water weresufficient to remove microbial contaminants during the introduction process. However, in some cases, cefotaxime was particularly effective for removing bacterial contamination. We found that antioxidant treatments of glutathione, glycine betaine, and ascorbic acid did not improve regrowth after liquid nitrogen exposure usine either PVS2 or PVS3 as cryoprotectants in droplet vitrification cryopreservation methods. Exposure durations of PVS2 and PVS3 were optimized, with and without exposure to liquid nitrogen (LN), and shoot tip regrowth levels ranged from 0 to 37% after LN exposure. Published methods for encapsulation dehydration and V-plate vitrification cryopreservation procedures were tested to determine if acceptable results could be obtained. Using these methods, shoot tip regrowth levels ranged from 0 to 50% after LN exposure. We conclude that the sugarcane cryopreservation methods that we tested are not yet ready for implementation in the NPGS.
650 $aCana de Açúcar
700 1 $aJENDEREK, M. M.
700 1 $aSTAATS, E.
700 1 $aSHEPHERD, A.
700 1 $aBONNART, R.
700 1 $aLEDO, A. da S.
700 1 $aAYALA SILVA. T.
773 $tActa Hortciulturae, 1234, 2019.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
04/08/2011 |
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Data da última atualização: |
28/05/2018 |
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Tipo da produção científica: |
Artigo de Divulgação na Mídia |
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Autoria: |
CRUZ, J. C.; PEREIRA FILHO, I. A.; SILVA, G. H. da. |
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Afiliação: |
JOSE CARLOS CRUZ, CNPMS; ISRAEL ALEXANDRE PEREIRA FILHO, CNPMS; GUSTAVO HENRIQUE DA SILVA, BOLSISTA. |
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Título: |
Mais de 170 cultivares transgênicas são disponibilizadas no mercado de sementes do Brasil para a safra 2011/12. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
APPS - Associação Paulista dos Produtores de Sementes e Mudas, São José Rio Preto, 4 nov. 2011. |
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Idioma: |
Português |
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Thesagro: |
Milho; Variedade; Zea Mays. |
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Thesaurus NAL: |
Varieties. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/39191/1/mais-170.pdf
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Marc: |
LEADER 00593nam a2200169 a 4500
001 1897287
005 2018-05-28
008 2011 bl uuuu u00u1 u #d
100 1 $aCRUZ, J. C.
245 $aMais de 170 cultivares transgênicas são disponibilizadas no mercado de sementes do Brasil para a safra 2011/12.$h[electronic resource]
260 $aAPPS - Associação Paulista dos Produtores de Sementes e Mudas, São José Rio Preto, 4 nov. 2011.$c2011
650 $aVarieties
650 $aMilho
650 $aVariedade
650 $aZea Mays
700 1 $aPEREIRA FILHO, I. A.
700 1 $aSILVA, G. H. da
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Embrapa Milho e Sorgo (CNPMS) |
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