Data/hora: 21/09/2024 12:16:12

Biblioteca(s): 	Embrapa Instrumentação.	
Data corrente: 	08/06/2021	
Data da última atualização: 	10/06/2022	
Autoria: 	FACCHINATTO, W. M.; GALANTE, J.; MESQUITA, L.; SILVA, D. S.; SANTOS, D. M. dos; MORAES, T. B.; CAMPANA-FILHO, S. P.; COLNAGO, L. A.; SARMENTO, B.; NEVES, J. das.	
Afiliação: 	LUIZ ALBERTO COLNAGO, CNPDIA.	
Título: 	Clotrimazole-loaded N-(2-hydroxy)-propyl-3-trimethylammonium, O-palmitoyl chitosan nanoparticles for topical treatment of vulvovaginal candidiasis.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Acta Biomaterialia, v. 125, 2021.	
Páginas: 	312?321	
ISSN: 	172-7061	
DOI: 	https://doi.org/10.1016/j.actbio.2021.02.029	
Idioma: 	Inglês	
Conteúdo: 	Vulvovaginal candidiasis (VVC) represents a considerable health burden for women. Despite the availability of a significant array of antifungal drugs and topical products, the management of the infection is not always effective, and new approaches are needed. Here, we explored cationic N-(2-hydroxy)- propyl-3-trimethylammonium, O-palmitoyl chitosan nanoparticles (NPs) as carriers of clotrimazole (CLT) for the topical treatment of VVC. CLT-NPs with approximately 280 nm in diameter were obtained by selfassembly in water and subsequent stabilization by ionic crosslinking with tripolyphosphate. The nanosystem featured pH-independent sustained drug release up to 24 h, which affected both in vitro anti-Candida activity and cytotoxicity. The CLT-loaded nanostructured platform yielded favorable selectivity index values for a panel of standard strains and clinical isolates of Candida spp. and female genital tract cell lines (HEC-1-A, Ca Ski and HeLa), as compared to the free drug. CLT-NPs also improved in vitro drug permeability across HEC-1-A and Ca Ski cell monolayers, thus suggesting that the nanocarrier may provide higher mucosal tissue levels of the active compound. Overall, data support that CLT-NPs may be a valuable asset for the topical treatment of VVC.	
Palavras-Chave: 	Health; Vaginal drug delivery; Women s health.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Instrumentação (CNPDIA)	
Exemplares: 	CNPDIA - (17783-1) - UPC - AP - DD - PROCI.21/43 -  - 2021/43 - DIS