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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
24/05/2021 |
Data da última atualização: |
24/05/2021 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
PRADO, G. S.; PINHEIRO, T. T.; FARIA, J. C. de; VIANELLO, R. P. |
Afiliação: |
GUILHERME SOUZA PRADO, especialista visitante CNPAF; THAÍSA TESSUTTI PINHEIRO, especialista visitante CNPAF; JOSIAS CORREA DE FARIA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
Título: |
Genome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP). |
Título original: |
Tecnologia CRISPR na edição genômica de plantas: biotecnologia aplicada à agricultura, 2020. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
In: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021. |
Páginas: |
p. 47-88. |
Descrição Física: |
il. |
ISBN: |
978-65-87380-10-0 |
Idioma: |
Inglês |
Conteúdo: |
We will focus here on the use of the CRISPR/Cas system to induce mutations via NHEJ. The NHEJ mechanism acts in most of the cell cycle and is not based on homology to repair DNA, causing mutations such as insertions or deletions (indels) or even substitutions. In this way, the expression of the genes in the place where the repair occurred by NHEJ can, in general, be interrupted, and the encoded proteins may not be functional. To clarify the process of genome editing by CRISPR, this chapter aims to address the details of the NHEJ mechanism, as well as the use of ribonucleoprotein systems (RNPs) to promote editing, in addition to providing a practical procedure for the design of experiments. |
Palavras-Chave: |
CRISPR; CRISPRevolution; Non-homologous. |
Thesagro: |
Biotecnologia; DNA; Engenharia Genética; Genoma; RNA. |
Thesaurus Nal: |
Biotechnology; Genetic engineering; Nucleases; Plant genetics; Ribonucleoproteins; RNA editing. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/223400/1/chap2.pdf
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Marc: |
LEADER 01908naa a2200361 a 4500 001 2131989 005 2021-05-24 008 2021 bl uuuu u00u1 u #d 020 $a978-65-87380-10-0 100 1 $aPRADO, G. S. 240 $aTecnologia CRISPR na edição genômica de plantas: biotecnologia aplicada à agricultura, 2020. 245 $aGenome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP).$h[electronic resource] 260 $c2021 300 $ap. 47-88.$cil. 520 $aWe will focus here on the use of the CRISPR/Cas system to induce mutations via NHEJ. The NHEJ mechanism acts in most of the cell cycle and is not based on homology to repair DNA, causing mutations such as insertions or deletions (indels) or even substitutions. In this way, the expression of the genes in the place where the repair occurred by NHEJ can, in general, be interrupted, and the encoded proteins may not be functional. To clarify the process of genome editing by CRISPR, this chapter aims to address the details of the NHEJ mechanism, as well as the use of ribonucleoprotein systems (RNPs) to promote editing, in addition to providing a practical procedure for the design of experiments. 650 $aBiotechnology 650 $aGenetic engineering 650 $aNucleases 650 $aPlant genetics 650 $aRibonucleoproteins 650 $aRNA editing 650 $aBiotecnologia 650 $aDNA 650 $aEngenharia Genética 650 $aGenoma 650 $aRNA 653 $aCRISPR 653 $aCRISPRevolution 653 $aNon-homologous 700 1 $aPINHEIRO, T. T. 700 1 $aFARIA, J. C. de 700 1 $aVIANELLO, R. P. 773 $tIn: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021.
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Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
19/09/2022 |
Data da última atualização: |
22/09/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 4 |
Autoria: |
TÁVORA, G. S. G.; TURETTA, A. P. D.; SILVA, A. S. da; SIMÕES, B. F. T.; NEHREN, U. |
Afiliação: |
GABRIEL SPÍNOLA GARCIA TÁVORA, UNIVERSIDADE DO ESTADO DO RIO DE JANEIRO; ANA PAULA DIAS TURETTA, CNPS; ANTONIO SOARES DA SILVA, UNIVERSIDADE DO ESTADO DO RIO DE JANEIRO; BRUNO FRANCISCO TEIXEIRA SIMÕES, UNIVERSIDADE FEDERAL DO ESTADO DO RIO DE JANEIRO; UDO NEHREN, UNIVERSITY OF APPLIED SCIENCES TH KÖLN. |
Título: |
Trade-offs and synergies in agricultural landscapes: a study on soil-related ecosystem services in the Brazilian Atlantic rainforest. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Environmental and Sustainability Indicators, v. 16, 100205, Dec. 2022. |
DOI: |
https://doi.org/10.1016/j.indic.2022.100205 |
Idioma: |
Inglês |
Conteúdo: |
In recent years, numerous studies have addressed the theme of ecosystem services as a means of promoting the protection, sustainable use, and recovery of ecosystems. However, these studies mainly have not been fully evaluated the soil-related ecosystem services and the different land uses and land cover. Taking this as a background, our main goal is to evaluate the trade-offs and synergies in the provision of soil-related ecosystem services in a watershed located in the highlands of Rio de Janeiro state - Brazil. To do so, we evaluated soil properties as indicators. In addition, statistical methods were applied to analyze any significant differences between the variables for the different land use classes, and Spearman correlation matrix to evaluate the trade-offs and synergies. No significant difference (p > 0.05) was found between the different land uses for soil fertility parameters, bulk density, and organic matter; however, the trade-offs analysis demonstrated the impact of anthropogenic actions in ecosystem services provision. The methodology showed potential to be used in different studies that focus on ecosystem services evaluations. |
Palavras-Chave: |
Carbono do solo; Nutrients regulation; Propriedades do solo; Regulação de nutrientes; Soil carbon. |
Thesagro: |
Agricultura Sustentável; Uso da Terra. |
Thesaurus NAL: |
Land use; Soil properties; Sustainable agriculture. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146606/1/Trade-offs-and-synergies-in-agricultural-landscapes-2022.pdf
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Marc: |
LEADER 02150naa a2200301 a 4500 001 2146606 005 2022-09-22 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.indic.2022.100205$2DOI 100 1 $aTÁVORA, G. S. G. 245 $aTrade-offs and synergies in agricultural landscapes$ba study on soil-related ecosystem services in the Brazilian Atlantic rainforest.$h[electronic resource] 260 $c2022 520 $aIn recent years, numerous studies have addressed the theme of ecosystem services as a means of promoting the protection, sustainable use, and recovery of ecosystems. However, these studies mainly have not been fully evaluated the soil-related ecosystem services and the different land uses and land cover. Taking this as a background, our main goal is to evaluate the trade-offs and synergies in the provision of soil-related ecosystem services in a watershed located in the highlands of Rio de Janeiro state - Brazil. To do so, we evaluated soil properties as indicators. In addition, statistical methods were applied to analyze any significant differences between the variables for the different land use classes, and Spearman correlation matrix to evaluate the trade-offs and synergies. No significant difference (p > 0.05) was found between the different land uses for soil fertility parameters, bulk density, and organic matter; however, the trade-offs analysis demonstrated the impact of anthropogenic actions in ecosystem services provision. The methodology showed potential to be used in different studies that focus on ecosystem services evaluations. 650 $aLand use 650 $aSoil properties 650 $aSustainable agriculture 650 $aAgricultura Sustentável 650 $aUso da Terra 653 $aCarbono do solo 653 $aNutrients regulation 653 $aPropriedades do solo 653 $aRegulação de nutrientes 653 $aSoil carbon 700 1 $aTURETTA, A. P. D. 700 1 $aSILVA, A. S. da 700 1 $aSIMÕES, B. F. T. 700 1 $aNEHREN, U. 773 $tEnvironmental and Sustainability Indicators$gv. 16, 100205, Dec. 2022.
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