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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Unidades Centrais. |
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Data corrente: |
20/09/2023 |
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Data da última atualização: |
27/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, J. A. V. de; VIANELLO, R. P.; LANNA, A. C.; DEDICOVA, B.; ROCHA, D. C.; BRONDANI, C. |
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Afiliação: |
JOÃO AUGUSTO VIEIRA DE OLIVEIRA, ADVANCED BACTERIAL SCIENCES Ltda.; ROSANA PEREIRA VIANELLO, CNPAF; ANNA CRISTINA LANNA, CNPAF; BEATA DEDICOVA, SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES; DHIÔVANNA CORRÊIA ROCHA, INSTITUTO AGRONÔMICO DE CAMPINAS; CLAUDIO BRONDANI, CNPAF. |
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Título: |
Drought tolerance induced by the overexpression of the nuclear rbcL gene in rice. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, v. 58, e03181, 2023. |
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ISSN: |
1678-3921 |
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DOI: |
https://doi.org/10.1590/S1678-3921.pab2023.v58.03181 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT - The objective of this work was to determine whether the overexpression of the nuclear Rubisco large subunit (rbcL) improves the drought tolerance of the genetically modified (GM) BRSMG Curinga upland rice (Oryza sativa) cultivar. GM and non-genetically modified (NGM) plants of the same cultivar were compared under the two following water treatments: well watered (WW) and water deficit (WD). The performance of the agronomic traits of GM plants, including grain yield, was superior to that of NGM plants in both treatments. By quantitative polymerase chain reaction, GM plants show a significantly higher expression of the rbcL gene in both WW and WD, as well as a larger amount of abscisic acid. With the RNAseq analysis, almost three times more upregulated genes are identified in GM plants in stage 2 after water restriction, indicating a greater protection against water deficit. The higher expression of genes related to the protection of the cellular metabolism and a series of physiological alterations may be involved in the increase in the drought tolerance of GM rice plants overexpressing the rbcL gene.
RESUMO - O objetivo deste trabalho foi determinar se a superexpressão da grande subunidade nuclear da Rubisco (rbcL) aumenta a tolerância à seca da
cultivar geneticamente modificada (GM) BRSMG Curinga de arroz (Oryza sativa) de terras altas. Plantas GMs e não geneticamente modificadas (NGMs)
da mesma cultivar foram comparadas nos dois seguintes tratamentos de água: irrigação (WW) e déficit hídrico (WD). O desempenho dos caracteres
agronômicos das plantas GMs, incluindo a produção de grãos, foi superior ao das NGMs em ambos os tratamentos. Por reação em cadeia da polimerase
quantitativa, plantas GMs apresentam expressão significativamente maior do gene rbcL, tanto sob WW como sob WD, além de maior quantidade de ácido
abscísico. Com a análise de RNAseq, quase três vezes mais genes regulados positivamente são identificados nas plantas GMs no estágio 2 após restrição
hídrica, o que indica maior proteção contra o déficit hídrico. O aumento da expressão de genes relacionados à proteção do metabolismo celular e uma série de alterações fisiológicas podem estar envolvidos no aumento da tolerância à seca em plantas de arroz GMs que superexpressam o gene rbcL. MenosABSTRACT - The objective of this work was to determine whether the overexpression of the nuclear Rubisco large subunit (rbcL) improves the drought tolerance of the genetically modified (GM) BRSMG Curinga upland rice (Oryza sativa) cultivar. GM and non-genetically modified (NGM) plants of the same cultivar were compared under the two following water treatments: well watered (WW) and water deficit (WD). The performance of the agronomic traits of GM plants, including grain yield, was superior to that of NGM plants in both treatments. By quantitative polymerase chain reaction, GM plants show a significantly higher expression of the rbcL gene in both WW and WD, as well as a larger amount of abscisic acid. With the RNAseq analysis, almost three times more upregulated genes are identified in GM plants in stage 2 after water restriction, indicating a greater protection against water deficit. The higher expression of genes related to the protection of the cellular metabolism and a series of physiological alterations may be involved in the increase in the drought tolerance of GM rice plants overexpressing the rbcL gene.
RESUMO - O objetivo deste trabalho foi determinar se a superexpressão da grande subunidade nuclear da Rubisco (rbcL) aumenta a tolerância à seca da
cultivar geneticamente modificada (GM) BRSMG Curinga de arroz (Oryza sativa) de terras altas. Plantas GMs e não geneticamente modificadas (NGMs)
da mesma cultivar foram comparadas nos dois seguintes tratamentos de água: ir... Mostrar Tudo |
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Palavras-Chave: |
Cultivar BRSMG Curinga; Déficit hídrico. |
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Thesagro: |
Ácido Abscisico; Características Agronômicas; Engenharia Genética; Irrigação; Melhoramento Genético Vegetal; Oryza Sativa; Resistência a Seca. |
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Thesaurus Nal: |
Abscisic acid; Drought tolerance; Genetic engineering; Rice. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156807/1/pab-2023-v58.pdf
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Marc: |
LEADER 03426naa a2200361 a 4500
001 2156807
005 2023-09-27
008 2023 bl uuuu u00u1 u #d
022 $a1678-3921
024 7 $ahttps://doi.org/10.1590/S1678-3921.pab2023.v58.03181$2DOI
100 1 $aOLIVEIRA, J. A. V. de
245 $aDrought tolerance induced by the overexpression of the nuclear rbcL gene in rice.$h[electronic resource]
260 $c2023
520 $aABSTRACT - The objective of this work was to determine whether the overexpression of the nuclear Rubisco large subunit (rbcL) improves the drought tolerance of the genetically modified (GM) BRSMG Curinga upland rice (Oryza sativa) cultivar. GM and non-genetically modified (NGM) plants of the same cultivar were compared under the two following water treatments: well watered (WW) and water deficit (WD). The performance of the agronomic traits of GM plants, including grain yield, was superior to that of NGM plants in both treatments. By quantitative polymerase chain reaction, GM plants show a significantly higher expression of the rbcL gene in both WW and WD, as well as a larger amount of abscisic acid. With the RNAseq analysis, almost three times more upregulated genes are identified in GM plants in stage 2 after water restriction, indicating a greater protection against water deficit. The higher expression of genes related to the protection of the cellular metabolism and a series of physiological alterations may be involved in the increase in the drought tolerance of GM rice plants overexpressing the rbcL gene. RESUMO - O objetivo deste trabalho foi determinar se a superexpressão da grande subunidade nuclear da Rubisco (rbcL) aumenta a tolerância à seca da cultivar geneticamente modificada (GM) BRSMG Curinga de arroz (Oryza sativa) de terras altas. Plantas GMs e não geneticamente modificadas (NGMs) da mesma cultivar foram comparadas nos dois seguintes tratamentos de água: irrigação (WW) e déficit hídrico (WD). O desempenho dos caracteres agronômicos das plantas GMs, incluindo a produção de grãos, foi superior ao das NGMs em ambos os tratamentos. Por reação em cadeia da polimerase quantitativa, plantas GMs apresentam expressão significativamente maior do gene rbcL, tanto sob WW como sob WD, além de maior quantidade de ácido abscísico. Com a análise de RNAseq, quase três vezes mais genes regulados positivamente são identificados nas plantas GMs no estágio 2 após restrição hídrica, o que indica maior proteção contra o déficit hídrico. O aumento da expressão de genes relacionados à proteção do metabolismo celular e uma série de alterações fisiológicas podem estar envolvidos no aumento da tolerância à seca em plantas de arroz GMs que superexpressam o gene rbcL.
650 $aAbscisic acid
650 $aDrought tolerance
650 $aGenetic engineering
650 $aRice
650 $aÁcido Abscisico
650 $aCaracterísticas Agronômicas
650 $aEngenharia Genética
650 $aIrrigação
650 $aMelhoramento Genético Vegetal
650 $aOryza Sativa
650 $aResistência a Seca
653 $aCultivar BRSMG Curinga
653 $aDéficit hídrico
700 1 $aVIANELLO, R. P.
700 1 $aLANNA, A. C.
700 1 $aDEDICOVA, B.
700 1 $aROCHA, D. C.
700 1 $aBRONDANI, C.
773 $tPesquisa Agropecuária Brasileira$gv. 58, e03181, 2023.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Milho e Sorgo. |
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Data corrente: |
21/06/2021 |
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Data da última atualização: |
17/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OLIVEIRA, I. C. M.; ANDRADE, C. de L. T. de; BORGES JÚNIOR, J. C. F.; SIMEÃO, R. M.; SILVA, A. P. C. M. |
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Afiliação: |
ISABELA CRISTINA MARTINS OLIVEIRA, Universidade Federal de São João del-Rei; CAMILO DE LELIS TEIXEIRA DE ANDRADE, CNPMS; JOÃO CARLOS FERREIRA BORGES JÚNIOR, Universidade Federal de São João del-Rei; ROSANGELA MARIA SIMEAO, CNPGC; ANA PAULA COELHO MADEIRA SILVA, Universidade Federal de São João del-Rei. |
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Título: |
Technical feasibility of using suboptimal irrigation in maize cropping. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Crop & Pasture Science, v. 72, p. 348-360, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
Maize (Zea mays L.) is one of the main cereal crops grown around the world and is widely used as food, feed and raw material in various industries. However, the crop is sensitive to weather conditions, and this causes large variations in yield between crop seasons. Suboptimal irrigation can be used to secure reasonable yields with increased water productivity. For maize grown offseason in the Cerrado biome of Brazil, there is little knowledge about the benefits of suboptimal irrigation. This study was designed to evaluate the effect of different irrigation amounts and two Brachiaria cultivars intercropped with maize on maize grain yield, biomass production for silage and water productivity in the second crop season. The study was conducted at Embrapa Milho e Sorgo Experimental Station, Sete Lagoas, MG, Brazil, in 2018 and 2019. Six irrigation levels were obtained by using a line-source sprinkler irrigation system. The irrigation levels used ranged from 94% to 11% of the cumulative reference evapotranspiration (ET0) in 2018, and from 90% to 34% of ET0 in 2019. Suboptimal irrigation above a certain level did not cause a significant decrease in silage biomass (59% and 71% of the ET0, in 2018 and 2019, respectively) and grain productivity (48% and 60% of the ET0, in 2018 and 2019, respectively). Additionally, there was an increase in crop water productivity and irrigation water productivity, for biomass of both silage and grains. The results demonstrate the potential of suboptimal irrigation as a strategy to reduce water resource usage while maintaining acceptable productivity levels. MenosMaize (Zea mays L.) is one of the main cereal crops grown around the world and is widely used as food, feed and raw material in various industries. However, the crop is sensitive to weather conditions, and this causes large variations in yield between crop seasons. Suboptimal irrigation can be used to secure reasonable yields with increased water productivity. For maize grown offseason in the Cerrado biome of Brazil, there is little knowledge about the benefits of suboptimal irrigation. This study was designed to evaluate the effect of different irrigation amounts and two Brachiaria cultivars intercropped with maize on maize grain yield, biomass production for silage and water productivity in the second crop season. The study was conducted at Embrapa Milho e Sorgo Experimental Station, Sete Lagoas, MG, Brazil, in 2018 and 2019. Six irrigation levels were obtained by using a line-source sprinkler irrigation system. The irrigation levels used ranged from 94% to 11% of the cumulative reference evapotranspiration (ET0) in 2018, and from 90% to 34% of ET0 in 2019. Suboptimal irrigation above a certain level did not cause a significant decrease in silage biomass (59% and 71% of the ET0, in 2018 and 2019, respectively) and grain productivity (48% and 60% of the ET0, in 2018 and 2019, respectively). Additionally, there was an increase in crop water productivity and irrigation water productivity, for biomass of both silage and grains. The results demonstrate the potential of suboptim... Mostrar Tudo |
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Palavras-Chave: |
Escassez de água; Sustentabilidade. |
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Thesagro: |
Água; Brachiaria; Consorciação de Cultura; Irrigação; Manejo; Milho; Produtividade; Zea Mays. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02411naa a2200289 a 4500
001 2132421
005 2022-11-17
008 2021 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, I. C. M.
245 $aTechnical feasibility of using suboptimal irrigation in maize cropping.$h[electronic resource]
260 $c2021
520 $aMaize (Zea mays L.) is one of the main cereal crops grown around the world and is widely used as food, feed and raw material in various industries. However, the crop is sensitive to weather conditions, and this causes large variations in yield between crop seasons. Suboptimal irrigation can be used to secure reasonable yields with increased water productivity. For maize grown offseason in the Cerrado biome of Brazil, there is little knowledge about the benefits of suboptimal irrigation. This study was designed to evaluate the effect of different irrigation amounts and two Brachiaria cultivars intercropped with maize on maize grain yield, biomass production for silage and water productivity in the second crop season. The study was conducted at Embrapa Milho e Sorgo Experimental Station, Sete Lagoas, MG, Brazil, in 2018 and 2019. Six irrigation levels were obtained by using a line-source sprinkler irrigation system. The irrigation levels used ranged from 94% to 11% of the cumulative reference evapotranspiration (ET0) in 2018, and from 90% to 34% of ET0 in 2019. Suboptimal irrigation above a certain level did not cause a significant decrease in silage biomass (59% and 71% of the ET0, in 2018 and 2019, respectively) and grain productivity (48% and 60% of the ET0, in 2018 and 2019, respectively). Additionally, there was an increase in crop water productivity and irrigation water productivity, for biomass of both silage and grains. The results demonstrate the potential of suboptimal irrigation as a strategy to reduce water resource usage while maintaining acceptable productivity levels.
650 $aÁgua
650 $aBrachiaria
650 $aConsorciação de Cultura
650 $aIrrigação
650 $aManejo
650 $aMilho
650 $aProdutividade
650 $aZea Mays
653 $aEscassez de água
653 $aSustentabilidade
700 1 $aANDRADE, C. de L. T. de
700 1 $aBORGES JÚNIOR, J. C. F.
700 1 $aSIMEÃO, R. M.
700 1 $aSILVA, A. P. C. M.
773 $tCrop & Pasture Science$gv. 72, p. 348-360, 2021.
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