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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
06/10/2008 |
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Data da última atualização: |
11/12/2023 |
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Autoria: |
GUNTER, L. E.; TUSKAN, G. A.; WULLSCHLEGER, S. D. |
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Título: |
Diversity among populations of switchgrass based on RAPD markers. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Crop Science, v. 36, n. 4, p. 1017-1022, Jul./Aug. 1996. |
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DOI: |
https://doi.org/10.2135/cropsci1996.0011183X003600040034x |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract; Information on the amount of genetic diversity in switchgrass (Panicum virgatum L.) is necessary to enhance the effectiveness of breeding programs and germplasm conservation efforts. This study characterized and assessed genetic diversity by means of RAPD markers among 14 populations representing upland and lowland switchgrass ecotypes. Forty-five of 128 primers produced polymorphic markers among sets of genomic DNA pooled from individual genotypes of each population. Five primers were selected to amplify a total of 91 polymorphic loci among genotypes. The RAPD markers were scored for presence or absence of bands to generate distance matrices for cluster analysis. Overall similarity was 65% among populations compared to 81% within populations. Blackwell and Caddo were the most similar populations (78%) based on RAPD markers, whereas Alamo and Forestburg were the most divergent (53%). Cluster analysis clearly segregated populations into two main groups (putatively based on ecotype) and united individual genotypes within a population into discrete groups within the larger clusters. Although the relationship between ploidy level and ecotype remained unclear, RAPD profiles can be used to identify switchgrass populations and may be useful in predicting relationships between experimental germplasm sources and released populations. |
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Palavras-Chave: |
Forrageira; Pannicum virgatum; RAPD. |
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Thesagro: |
Marcador Molecular; Variação Genética. |
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Thesaurus Nal: |
Forage grasses; Genetic markers; Genetic variation; Panicum virgatum; Plant genetics; Random amplified polymorphic DNA technique. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02220naa a2200289 a 4500
001 1534301
005 2023-12-11
008 1996 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.2135/cropsci1996.0011183X003600040034x$2DOI
100 1 $aGUNTER, L. E.
245 $aDiversity among populations of switchgrass based on RAPD markers.
260 $c1996
520 $aAbstract; Information on the amount of genetic diversity in switchgrass (Panicum virgatum L.) is necessary to enhance the effectiveness of breeding programs and germplasm conservation efforts. This study characterized and assessed genetic diversity by means of RAPD markers among 14 populations representing upland and lowland switchgrass ecotypes. Forty-five of 128 primers produced polymorphic markers among sets of genomic DNA pooled from individual genotypes of each population. Five primers were selected to amplify a total of 91 polymorphic loci among genotypes. The RAPD markers were scored for presence or absence of bands to generate distance matrices for cluster analysis. Overall similarity was 65% among populations compared to 81% within populations. Blackwell and Caddo were the most similar populations (78%) based on RAPD markers, whereas Alamo and Forestburg were the most divergent (53%). Cluster analysis clearly segregated populations into two main groups (putatively based on ecotype) and united individual genotypes within a population into discrete groups within the larger clusters. Although the relationship between ploidy level and ecotype remained unclear, RAPD profiles can be used to identify switchgrass populations and may be useful in predicting relationships between experimental germplasm sources and released populations.
650 $aForage grasses
650 $aGenetic markers
650 $aGenetic variation
650 $aPanicum virgatum
650 $aPlant genetics
650 $aRandom amplified polymorphic DNA technique
650 $aMarcador Molecular
650 $aVariação Genética
653 $aForrageira
653 $aPannicum virgatum
653 $aRAPD
700 1 $aTUSKAN, G. A.
700 1 $aWULLSCHLEGER, S. D.
773 $tCrop Science$gv. 36, n. 4, p. 1017-1022, Jul./Aug. 1996.
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