Data/hora: 17/06/2024 00:43:53

Biblioteca(s): | Embrapa Semiárido.
Data corrente: | 19/02/2019
Data da última atualização: | 21/01/2020
Autoria: | SILVA, V. B. da; SILVA, A. F. da; SILVA, T. R. da; SANTOS, J. W. M. dos; SILVA, J. F. da; SOUZA, A. P. de; FREITAS, A. D. S. de; FERNANDES JUNIOR, P. I.
Afiliação: | VALÉRIA BORGES DA SILVA; ALEKSANDRO FERREIRA DA SILVA; THAISE ROSA DA SILVA; JONNATHAN WHINY MORAES DOS SANTOS; JÉSSICA FERNANDA DA SILVA; ADAILSON PEREIRA DE SOUZA; ANA DOLORES SANTIAGO DE FREITAS; PAULO IVAN FERNANDES JUNIOR, CPATSA.
Título: | Fast and efficient symbiotic gene-based duplex PCR approach for the preliminary selection of legume root nodule bacteria.
Ano de publicação: | 2019
Fonte/Imprenta: | Rhizosphere, v. 10, 2019.
ISSN: | 2452-2198
DOI: | 10.1016/j.rhisph.2019.100144
Idioma: | Inglês
Conteúdo: | The isolation of root nodule bacteria (RNB) usually lead to obtainment of several non-rhizobia, mainly those fastgrowing bacteria. The plant-authentication experiments to select the nodulating bacteria are time consuming and can be ruined in case of nodulation of negative controls. To speed up this step in rhizobiology research a fast, simple and efficient duplex PCR assay for the amplification of nodC and nifH genes in was developed aiming to separate rhizobia and non-rhizobia in RNB culture collections. The method was optimized with 43 reference strains and was applied to a new culture collection for validation. Considering all bacteria, 106 out of 109 nodulating rhizobia were positive for both genes. Among non-nodulating, 48 out of 48 were nodC-negative. The results demonstrated the efficiency of this new method that can be completed in a day, to save time and money in RNB isolation projects, fulfilling Koch's postulate, and separating rhizobia from non-rhizobial bacteria.
Palavras-Chave: | Koch's postulates; Nódulo da raiz.
Thesagro: | Bactéria; Leguminosa.
Thesaurus Nal: | Legumes.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Semiárido (CPATSA)
Exemplares: | CPATSA - (58049-1) - UPC - AP - DD -  -  -  - DIS