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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
19/09/2018 |
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Data da última atualização: |
28/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, L. L.; PESTANA, K. N.; FERREIRA, C. F.; OLIVEIRA, S. A. S. de. |
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Afiliação: |
LEANDRO L. SILVA, UFRB; KÁTIA N. PESTANA; CLAUDIA FORTES FERREIRA, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF. |
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Título: |
Differentiation of phylogenetic lineages within the 'Colletotrichum gloeosporioides species complex' associated with cassava anthracnose disease by PCR-RFLP. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v.43, p.194-20, 2018. |
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ISSN: |
1983-2052 |
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Idioma: |
Inglês |
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Conteúdo: |
Different species were found associated with cassava anthracnose, considered a major disease of this crop. The correct identification of the causal agent is a first step for defining appropriate control strategies, such as resistant varieties. In silico analyses used sequences of six genomic regions of ex-type specimens from the ?Colletotrichum gloeosporioides species complex? (C.g.SC) and 21 restriction enzymes to identify phylogenetic lineages. The three best combinations of region/enzymes were validated in 18 Colletotrichum spp. isolates from cassava. Dendrograms for in silico PCR-RFLP for CAL, ITS and TUB2 showed considerable agreement with the phylogenetic analysis of each genomic region; however, the CAL gene presented greater discriminatory power. Since the band patterns from in gel analysis were almost the same as expected for the in silico analysis for the CAL region, a new approach was proposed based on the combined data from these two methodologies, allowing the differentiation of five phylogenetic lineages within the C.g.SC (C. tropicale; C. fructicola; C. siamense; C. gloeosporioides sensu stricto and C. theobromicola), and one outside of this complex (C. cliviae). This evaluation showed to be a reliable technique for preliminary identification of species prior to sequencing. |
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Thesagro: |
Mandioca. |
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Thesaurus Nal: |
Cassava. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01935naa a2200193 a 4500
001 2096059
005 2018-09-28
008 2018 bl uuuu u00u1 u #d
022 $a1983-2052
100 1 $aSILVA, L. L.
245 $aDifferentiation of phylogenetic lineages within the 'Colletotrichum gloeosporioides species complex' associated with cassava anthracnose disease by PCR-RFLP.$h[electronic resource]
260 $c2018
520 $aDifferent species were found associated with cassava anthracnose, considered a major disease of this crop. The correct identification of the causal agent is a first step for defining appropriate control strategies, such as resistant varieties. In silico analyses used sequences of six genomic regions of ex-type specimens from the ?Colletotrichum gloeosporioides species complex? (C.g.SC) and 21 restriction enzymes to identify phylogenetic lineages. The three best combinations of region/enzymes were validated in 18 Colletotrichum spp. isolates from cassava. Dendrograms for in silico PCR-RFLP for CAL, ITS and TUB2 showed considerable agreement with the phylogenetic analysis of each genomic region; however, the CAL gene presented greater discriminatory power. Since the band patterns from in gel analysis were almost the same as expected for the in silico analysis for the CAL region, a new approach was proposed based on the combined data from these two methodologies, allowing the differentiation of five phylogenetic lineages within the C.g.SC (C. tropicale; C. fructicola; C. siamense; C. gloeosporioides sensu stricto and C. theobromicola), and one outside of this complex (C. cliviae). This evaluation showed to be a reliable technique for preliminary identification of species prior to sequencing.
650 $aCassava
650 $aMandioca
700 1 $aPESTANA, K. N.
700 1 $aFERREIRA, C. F.
700 1 $aOLIVEIRA, S. A. S. de
773 $tTropical Plant Pathology$gv.43, p.194-20, 2018.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
10/01/2005 |
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Data da última atualização: |
28/10/2009 |
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Autoria: |
SILVA, E. M. da; LIMA, J. E. F. W. |
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Título: |
Alternativas de captação de água visando ao aumento da oferta hídrica na Agrovila Mambaí- GO. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Planaltina, DF: Embrapa Cerrados, 2003. |
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Páginas: |
24 p. |
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Série: |
(Embrapa Cerrados. Documentos, 92). |
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Idioma: |
Português |
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Conteúdo: |
ABSTRACT: The water supply for human use in Brazilian rural areas of the Cerrado region depends greatly on local natural water resources. In the community of smallholder farmers, know as Agrovila Mambaí, located in Mambaí, GO, has been undergoing this type of situation. The objective of this work has been developed aiming at presenting technical alternatives to improve the current water system capacity to supply water the referred smallholder farmers. In older to support this analysis, there were used nearby hydrological data and digital map of the area to estimate the availability of water resources. Besides this, it was carried out local inventory to evaluate watershed conditions and the alternatives for improving the current water supply systems to increase the water availability for each smallholder farmer. It was concluded that it is feasible to increase the water supply for drinking usage by increasing the capacity of a local natural spring; installing a second piston-type water-pump driven by the installed water wheel; or an electrical motor-driven water pump to feed the existing elevated reservoir which currently is connected to water distribution system of the community. |
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Palavras-Chave: |
Brasil; Capatação de água; Captação d'água; Goiás; Mambaí; Water availability. |
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Thesagro: |
Abastecimento; Abastecimento de Água; Água; Água Potável; Cerrado; Disponibilidade de Água; Manejo de Água. |
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Thesaurus NAL: |
Brazil; water binding capacity; water supply. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAC-2009/25156/1/doc_92.pdf
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Marc: |
LEADER 02136nam a2200337 a 4500
001 1568175
005 2009-10-28
008 2003 bl uuuu u0uu1 u #d
100 1 $aSILVA, E. M. da
245 $aAlternativas de captação de água visando ao aumento da oferta hídrica na Agrovila Mambaí- GO.
260 $aPlanaltina, DF: Embrapa Cerrados$c2003
300 $a24 p.
490 $a(Embrapa Cerrados. Documentos, 92).
520 $aABSTRACT: The water supply for human use in Brazilian rural areas of the Cerrado region depends greatly on local natural water resources. In the community of smallholder farmers, know as Agrovila Mambaí, located in Mambaí, GO, has been undergoing this type of situation. The objective of this work has been developed aiming at presenting technical alternatives to improve the current water system capacity to supply water the referred smallholder farmers. In older to support this analysis, there were used nearby hydrological data and digital map of the area to estimate the availability of water resources. Besides this, it was carried out local inventory to evaluate watershed conditions and the alternatives for improving the current water supply systems to increase the water availability for each smallholder farmer. It was concluded that it is feasible to increase the water supply for drinking usage by increasing the capacity of a local natural spring; installing a second piston-type water-pump driven by the installed water wheel; or an electrical motor-driven water pump to feed the existing elevated reservoir which currently is connected to water distribution system of the community.
650 $aBrazil
650 $awater binding capacity
650 $awater supply
650 $aAbastecimento
650 $aAbastecimento de Água
650 $aÁgua
650 $aÁgua Potável
650 $aCerrado
650 $aDisponibilidade de Água
650 $aManejo de Água
653 $aBrasil
653 $aCapatação de água
653 $aCaptação d'água
653 $aGoiás
653 $aMambaí
653 $aWater availability
700 1 $aLIMA, J. E. F. W.
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