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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
21/03/1996 |
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Data da última atualização: |
03/05/2023 |
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Autoria: |
GUTIERREZ CANCELA, M. M.; GARCIA MARIN, J. F. |
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Título: |
Comparison of ziehl-neelsen staining and immunohistochemistry for the detection of mycobacterium bovis in bovine and caprine tuberculous lesions. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Journal of Comparative Pathology, v. 109, n. 4, p. 361-370, 1993. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The avidin-biotin complex peroxidase (ABC-P) method was used to detect Mycobacterium bovis, and the results were compared with those obtained by the Ziehl-Neelsen (ZN) technique. Lesions were examined from 18 cows and 24 goats with tuberculosis. All animals showed pulmonary lesions, which in the cattle were mainly minor (i.e. primary complex) but in the goats were sometimes minor and sometimes severe. Microscopically, typical granulomas were seen in the lungs and lymph nodes, with central necrosis and the cellular components of chronic inflammation, but mycobacteria were either seen in small numbers or were not detectable. The ABC-P technique was more sensitive than the ZN method, as shown by the number of positive animals detected, the intensity of staining, and the successful use of low magnification. Caprine lesions, although more severe than bovine lesions, appeared to contain fewer organisms. |
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Palavras-Chave: |
Imunohistoquimica; Labeling. |
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Thesagro: |
Bovino; Caprino; Diagnostico; Doença; Tuberculose. |
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Thesaurus Nal: |
Animal pathology; Bovine tuberculosis; Cattle diseases; Goat diseases; Goats; Immunohistochemistry; Microbiology; Staining. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01854naa a2200313 a 4500
001 1523500
005 2023-05-03
008 1993 bl uuuu u00u1 u #d
100 1 $aGUTIERREZ CANCELA, M. M.
245 $aComparison of ziehl-neelsen staining and immunohistochemistry for the detection of mycobacterium bovis in bovine and caprine tuberculous lesions.$h[electronic resource]
260 $c1993
520 $aAbstract: The avidin-biotin complex peroxidase (ABC-P) method was used to detect Mycobacterium bovis, and the results were compared with those obtained by the Ziehl-Neelsen (ZN) technique. Lesions were examined from 18 cows and 24 goats with tuberculosis. All animals showed pulmonary lesions, which in the cattle were mainly minor (i.e. primary complex) but in the goats were sometimes minor and sometimes severe. Microscopically, typical granulomas were seen in the lungs and lymph nodes, with central necrosis and the cellular components of chronic inflammation, but mycobacteria were either seen in small numbers or were not detectable. The ABC-P technique was more sensitive than the ZN method, as shown by the number of positive animals detected, the intensity of staining, and the successful use of low magnification. Caprine lesions, although more severe than bovine lesions, appeared to contain fewer organisms.
650 $aAnimal pathology
650 $aBovine tuberculosis
650 $aCattle diseases
650 $aGoat diseases
650 $aGoats
650 $aImmunohistochemistry
650 $aMicrobiology
650 $aStaining
650 $aBovino
650 $aCaprino
650 $aDiagnostico
650 $aDoença
650 $aTuberculose
653 $aImunohistoquimica
653 $aLabeling
700 1 $aGARCIA MARIN, J. F.
773 $tJournal of Comparative Pathology$gv. 109, n. 4, p. 361-370, 1993.
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