Data/hora: 15/06/2024 18:11:32

Biblioteca(s): | Embrapa Milho e Sorgo.
Data corrente: | 14/04/2009
Data da última atualização: | 30/05/2018
Autoria: | COELHO, M. R. R.; CARNEIRO, N. P.; MARRIEL, I. E.; SELDIN, L.
Afiliação: | Marcia Reed Rodrigues Coelho, UFRJ; NEWTON PORTILHO CARNEIRO, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS; Lucy Seldin, UFRJ.
Título: | Molecular detection of nifH gene-containing Paenibacillus in the rhizosphere of sorghum (Sorghum bicolor) sown in Cerrado soil.
Ano de publicação: | 2009
Fonte/Imprenta: | Letters in Applied Microbiology, Oxford, v. 48, n. 5, p. 611-617, 2009.
DOI: | 10.1111/j1472.765X.2009.02578.x
Idioma: | Inglês
Conteúdo: | Aims: To develop a polymerase chain reaction (PCR)-based approach for the detection of nifH gene-containing Paenibacillus in environmental samples. Methods and Results: The primers, nitHPAENf and nitHPAENr, were designed and tested with DNA from: (i) strains of different nitrogen-fixing Paenibacillus species, (ii) strains of other nitrogen-fixing genera and (iii) rhizosphere of sorghum sown in Cerrado soi! amended with either 12 or 120 kg ha-1 of nitrogen fertilizer. All nitrogen-fixing Paenibacillus strains tested and the DNA samples from rhizosphere soil were amplified when these primers were used, generating a 280 bp fragment. When the PCR products obtained from both sorghum rhizospheres were cloned and sequenced, the majority of the clones analysed could be identified as Paenibacillus durus. Moreover, a greater diversity in the nifH sequences could be observed in the rhizosphere treated with a high amount of nitrogen fertilizer. Conclusions: Nitrogen fertilization slightly influenced the structure of the nifH gene-containing Paenibacillus community in sorghum rhizospheres cultivated in Cerrado soil. Significance and Impact of the Study: The PCR detection method developed is adequate to assess the presence of nifH gene-containing Paenibacillus in the environment and can be used in future to determine the ecological role of this group of micro-organisms for the nitrogen input to the plants.
Palavras-Chave: | nifH; PCR; Primers; Sorghum.
Thesaurus Nal: | Paenibacillus.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Milho e Sorgo (CNPMS)
Exemplares: | CNPMS - (21604-1) - UPC - AP - DD -  -  -  - DIS