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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
10/01/2005 |
Data da última atualização: |
03/08/2017 |
Autoria: |
GRANGE, L.; HUNGRIA, M. |
Título: |
Genetic diversity of indigenous common bean (Phaseolus vulgaris) rhizobia in two Brazilian ecosystems. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Soil Biology and Biochemistry, Oxford, v. 36, n. 9, p. 1389-1398, Sept. 2004. |
Idioma: |
Inglês |
Conteúdo: |
Although rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two strains with identical profiles showed higher similarity to Sinorhizobium fredii. Other strains showed higher similarity of bases with R. tropici, R. leguminosarum and Mesorhizobium plurifarium, but some strains were quite dissimilar and may represent new species. Great variability was also verified among the sequenced strains in relation to the ability to grow in YMA at 40 °C, in LB, to synthesize melanin in vitro, as well as in symbiotic performance, including differences in relation to the described species, e.g. many R. etli strains were able to grow in LB and in YMA at 40 °C, and not all R. tropici were able to nodulate Leucaena. MenosAlthough rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two str... Mostrar Tudo |
Palavras-Chave: |
Rizóbio. |
Thesagro: |
Feijão. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02618naa a2200157 a 4500 001 1467760 005 2017-08-03 008 2004 bl --- 0-- u #d 100 1 $aGRANGE, L. 245 $aGenetic diversity of indigenous common bean (Phaseolus vulgaris) rhizobia in two Brazilian ecosystems. 260 $c2004 520 $aAlthough rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two strains with identical profiles showed higher similarity to Sinorhizobium fredii. Other strains showed higher similarity of bases with R. tropici, R. leguminosarum and Mesorhizobium plurifarium, but some strains were quite dissimilar and may represent new species. Great variability was also verified among the sequenced strains in relation to the ability to grow in YMA at 40 °C, in LB, to synthesize melanin in vitro, as well as in symbiotic performance, including differences in relation to the described species, e.g. many R. etli strains were able to grow in LB and in YMA at 40 °C, and not all R. tropici were able to nodulate Leucaena. 650 $aFeijão 653 $aRizóbio 700 1 $aHUNGRIA, M. 773 $tSoil Biology and Biochemistry, Oxford$gv. 36, n. 9, p. 1389-1398, Sept. 2004.
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Embrapa Soja (CNPSO) |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
15/04/2003 |
Data da última atualização: |
26/02/2020 |
Autoria: |
MEIRA, C. A. A.; SEIXAS NETO, Á.; NAKA, J.; CUNHA, L. M. S. |
Afiliação: |
CARLOS ALBERTO ALVES MEIRA, CNPTIA; ÁLVARO SEIXAS NETO, CNPTIA; JUAQUIM NAKA, MAPA; LUIZ MANOEL SILVA CUNHA, CNPTIA. |
Título: |
Análise de comércio exterior de frutas a partir do armazém de dados da fruticultura brasileira. |
Ano de publicação: |
2001 |
Fonte/Imprenta: |
Campinas: Embrapa Informática Agropecuária, 2001. |
Páginas: |
6 p. |
Série: |
(Embrapa Informática Agropecuária. Comunicado técnico, 2). |
ISSN: |
1677-8464 |
Idioma: |
Português |
Conteúdo: |
O objetivo geral do Programa é elevar os padrões de qualidade e competitividade da fruticultura brasileira ao patamar de excelência requerido pelo mercado internacional, paradigma que será efetivado com o avanço da capacidade produtiva e gerencial dos produtores de frutas e a ampliação dos mercados interno e externo. São contempladas ações em diversas áreas, como inovação tecnológica, capacitação de fruticultores, controle de pragas na fruticultura, campanha para promoção das frutas brasileiras, entre outras. |
Palavras-Chave: |
Competitividade; Inovação tecnológica; New technology. |
Thesagro: |
Fruta; Fruticultura; Mercado Internacional; Qualidade. |
Thesaurus NAL: |
Fruit growing. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPTIA/9175/1/COMNICADOTEC2.pdf
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Marc: |
LEADER 01371nam a2200277 a 4500 001 1001984 005 2020-02-26 008 2001 bl uuuu u0uu1 u #d 022 $a1677-8464 100 1 $aMEIRA, C. A. A. 245 $aAnálise de comércio exterior de frutas a partir do armazém de dados da fruticultura brasileira.$h[electronic resource] 260 $aCampinas: Embrapa Informática Agropecuária$c2001 300 $a6 p. 490 $a(Embrapa Informática Agropecuária. Comunicado técnico, 2). 520 $aO objetivo geral do Programa é elevar os padrões de qualidade e competitividade da fruticultura brasileira ao patamar de excelência requerido pelo mercado internacional, paradigma que será efetivado com o avanço da capacidade produtiva e gerencial dos produtores de frutas e a ampliação dos mercados interno e externo. São contempladas ações em diversas áreas, como inovação tecnológica, capacitação de fruticultores, controle de pragas na fruticultura, campanha para promoção das frutas brasileiras, entre outras. 650 $aFruit growing 650 $aFruta 650 $aFruticultura 650 $aMercado Internacional 650 $aQualidade 653 $aCompetitividade 653 $aInovação tecnológica 653 $aNew technology 700 1 $aSEIXAS NETO, Á. 700 1 $aNAKA, J. 700 1 $aCUNHA, L. M. S.
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