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Registros recuperados : 112 | |
42. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FIGUEIREDO, N. L. de A.; BATISTA, S. C. de O.; BALDANI, J. I.; SCHWAB, S. Caracterização de promotores de transcrição de Gluconacetobacter diazotrophicus regulados em resposta a caldo de variedades comercial e selvagem de cana-de-açúcar. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO, 18., 2008, Seropédica, RJ. Seropédica, RJ, 2008. Biblioteca(s): Embrapa Agrobiologia. |
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43. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SOUZA, M. L. da C.; SCHWAB, S.; BALDANI, J. I.; ROUWS, L. F. M. Marcação de Bradyrhizobium spp. com proteína verde fluorescente. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 19, 2019, Seropédica, RJ. Bioeconomia, diversidade e riqueza para o desenvolvimento sustentável. Caderno de resumos... Seropédica: Embrapa Agrobiologia, 2019. Biblioteca(s): Embrapa Agrobiologia. |
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44. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | GUEDES, H. V.; ROUWS, L. F. M.; SCHWAB, S.; SIMÕES-ARAÚJO, J. L.; BALDANI, J. I. A novel histidine kinase of Gluconacetobacter diazotrophicus might be involved in nitrogen fixation. In: INTERNATIONAL SYMPOSIUM ON NITROGEN FIXATION WITH NON-LEGUMES, 11th., 2008, Gent, Belgium. Abstracts... Belgium: Universiteit Gent, 2008. p. 9. Resumo PS1-5. Parceria: UFRRJ. Biblioteca(s): Embrapa Agrobiologia. |
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48. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FAZA, A. C. P.; GITAHY, P. de M.; SCHWAB, S.; BALDANI, J. I. Expressão do gene cry1Ab de Bacillus thuringiensis subespécie kurstaki estirpe S76 na bactéria endofítica Gluconacetobacter diazotrophicus para aplicações biotecnológicas. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO, 18., 2008, Seropédica, RJ. Seropédica, RJ, 2008 Parceria: UFRRJ Biblioteca(s): Embrapa Agrobiologia. |
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59. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | CAMARGO, A. B. de; SCHWAB, S.; REIS, V. M.; BALDANI, J. I. Transformação por eletroporação de Herbaspirillum seropedicae HRC54, presente no inoculante recomendado para cana-de-açúcar, com gene repórter DsRED. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 10., 18 a 24 de outubro de 2010, Seropédica. Ciência para o Desenvolvimento Sustentável: anais... Seropédica: Embrapa Agrobiologia, 2010. Biblioteca(s): Embrapa Agrobiologia. |
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Registros recuperados : 112 | |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
13/08/2019 |
Data da última atualização: |
26/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
FRANCISCO, R.; BRANCO, R.; SCHWAB, S.; BALDANI, J. I.; MORAIS, P. V. |
Afiliação: |
Romeu Francisco, University of Coimbra; Rita Branco, University of Coimbra; STEFAN SCHWAB, CNPAB; JOSE IVO BALDANI, CNPAB; Paula V. Morais1,2, BOLSISTA EMBRAPA AGROBIOLOGIA. |
Título: |
Two plant hosted whole cell bacterial biosensors for detection of bioavailable Cr(VI). |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 35, n. p. 129-143, 2019. |
DOI: |
https://doi.org/10.1007/s11274-019-2703-0 |
Idioma: |
Inglês |
Conteúdo: |
Metal whole cell biosensors (WCBs) have been reported as very useful tools to detect and quantify the presence of bioavailable fractions of certain metals in water and soil samples. In the current work, two bacterial WCBs able to report Cr(VI) presence and plants growing on Cr(VI)-enriched soil/medium were used to assess the potential transfer of this metal to organisms of higher trophic levels, and the risk of transfer to the food chain. To do it, the functionality of the WCBs within tissues of inoculated plants in contact with Cr(VI)-contaminated soil and water was studied in vitro and in a controlled greenhouse environment. One WCB was the previously described Ochrobactrum tritici pCHRGFP2 and the second, Nitrospirillum amazonense pCHRGFP2, is a newly engineered naturally-occurring endophytic microorganism. Three rice varieties (IAC 4440, BRS 6 CHUÍ, IRGA 425) and one maize variety (1060) were tested as hosts and subjected to Cr(VI) treatments (25 ?M), with different results obtained. Inoculation of each WCB into plants exposed to Cr(VI) showed GFP expression within plant tissues. WCBs penetrated the root tissues and later colonized the shoots and leaves. In general, a higher fluorescence signal was detected in roots, together with a higher Cr content and denser WCB colonization. Best fluorescence intensities per plant biomass of shoots were obtained for plant host IRGA 425. Therefore, by analyzing colonized tissues, both WCBs allowed the detection of Cr(VI) contamination in soils and its transfer to plants commonly used in crops for human diet. MenosMetal whole cell biosensors (WCBs) have been reported as very useful tools to detect and quantify the presence of bioavailable fractions of certain metals in water and soil samples. In the current work, two bacterial WCBs able to report Cr(VI) presence and plants growing on Cr(VI)-enriched soil/medium were used to assess the potential transfer of this metal to organisms of higher trophic levels, and the risk of transfer to the food chain. To do it, the functionality of the WCBs within tissues of inoculated plants in contact with Cr(VI)-contaminated soil and water was studied in vitro and in a controlled greenhouse environment. One WCB was the previously described Ochrobactrum tritici pCHRGFP2 and the second, Nitrospirillum amazonense pCHRGFP2, is a newly engineered naturally-occurring endophytic microorganism. Three rice varieties (IAC 4440, BRS 6 CHUÍ, IRGA 425) and one maize variety (1060) were tested as hosts and subjected to Cr(VI) treatments (25 ?M), with different results obtained. Inoculation of each WCB into plants exposed to Cr(VI) showed GFP expression within plant tissues. WCBs penetrated the root tissues and later colonized the shoots and leaves. In general, a higher fluorescence signal was detected in roots, together with a higher Cr content and denser WCB colonization. Best fluorescence intensities per plant biomass of shoots were obtained for plant host IRGA 425. Therefore, by analyzing colonized tissues, both WCBs allowed the detection of Cr(VI) contamination... Mostrar Tudo |
Palavras-Chave: |
Nitrospirillum amazonense; Wholecell biosensors. |
Thesaurus NAL: |
Chromium; Rice soils. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02293naa a2200229 a 4500 001 2111307 005 2019-11-26 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s11274-019-2703-0$2DOI 100 1 $aFRANCISCO, R. 245 $aTwo plant hosted whole cell bacterial biosensors for detection of bioavailable Cr(VI).$h[electronic resource] 260 $c2019 520 $aMetal whole cell biosensors (WCBs) have been reported as very useful tools to detect and quantify the presence of bioavailable fractions of certain metals in water and soil samples. In the current work, two bacterial WCBs able to report Cr(VI) presence and plants growing on Cr(VI)-enriched soil/medium were used to assess the potential transfer of this metal to organisms of higher trophic levels, and the risk of transfer to the food chain. To do it, the functionality of the WCBs within tissues of inoculated plants in contact with Cr(VI)-contaminated soil and water was studied in vitro and in a controlled greenhouse environment. One WCB was the previously described Ochrobactrum tritici pCHRGFP2 and the second, Nitrospirillum amazonense pCHRGFP2, is a newly engineered naturally-occurring endophytic microorganism. Three rice varieties (IAC 4440, BRS 6 CHUÍ, IRGA 425) and one maize variety (1060) were tested as hosts and subjected to Cr(VI) treatments (25 ?M), with different results obtained. Inoculation of each WCB into plants exposed to Cr(VI) showed GFP expression within plant tissues. WCBs penetrated the root tissues and later colonized the shoots and leaves. In general, a higher fluorescence signal was detected in roots, together with a higher Cr content and denser WCB colonization. Best fluorescence intensities per plant biomass of shoots were obtained for plant host IRGA 425. Therefore, by analyzing colonized tissues, both WCBs allowed the detection of Cr(VI) contamination in soils and its transfer to plants commonly used in crops for human diet. 650 $aChromium 650 $aRice soils 653 $aNitrospirillum amazonense 653 $aWholecell biosensors 700 1 $aBRANCO, R. 700 1 $aSCHWAB, S. 700 1 $aBALDANI, J. I. 700 1 $aMORAIS, P. V. 773 $tWorld Journal of Microbiology and Biotechnology$gv. 35, n. p. 129-143, 2019.
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