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| Registros recuperados : 57 | |
| 22. |  | SCHOLZ, M. B. dos S.; KITZBERGER, C. S. G.; PEREIRA, L. F. P.; DAVRIEUX, F.; POT, D.; CHARMETANT, P.; LEROY, T. Application of near infrared spectroscopy for green coffee biochemical phenotyping. Journal of Near Infrared Spectroscopy, v. 22, n. 6, p. 411-421, 2014.| Biblioteca(s): Embrapa Café. |
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| 23. | | IVAMOTO, S. T.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; ANDRADE, D. S.; ALVES, L. C.; DOMINGUES, D. S.; VIEIRA, L. G. E.; PEREIRA, L. F. P. In silico analysis of cytochrome p450 genes involved in the metabolism of diterpenes in Coffea. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos...| Biblioteca(s): Embrapa Café. |
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| 24. | | RAKOCEVIC, M.; BATISTA, E. R.; PAZIANOTTO, R. A. A.; SCHOLZ, M. B. S.; SOUZA, G. A. R.; CAMPOSTRINI, E.; RAMALHO, J. C. Leaf gas exchange and bean quality fluctuations over the whole canopy vertical profile of Arabic coffee cultivated under elevated CO2. Functional Plant Biology, v. 48, n. 5, p. 469-482, 2021.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 25. | | GARRETT, R.; SCHMIDT, E. M.; PEREIRA, L. F. P.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; EBERLIN, M. N.; REZENDE, C. M. Discrimination of arabica coffee cultivars by electrospray ionization fourier transform ion cyclotron resonance mass spectrometry and chemometrics. LWT - Food Science and Technology, v. 50, n. 2, p. 496-502, 2013.| Biblioteca(s): Embrapa Café. |
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| 26. | | KITZBERGER, C. S. G.; SCHOLZ, M. B. dos S.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; SERA, T.; SILVA, J. B. G. D.; BENASSI, M. de T. Diterpenes in green and roasted coffee of Coffea arabica cultivars growing in the same edapho-climatic conditions. Journal of Food Composition and Analysis, v. 30, n. 1, P. 52-57, 2013.| Biblioteca(s): Embrapa Café. |
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| 27. | | SCHOLZ, M. B. dos S.; KITZBERGER, C. S. G.; PAGIATTO, N. F.; PEREIRA, L. F. P.; DAVRIEUX, F.; POT, D.; CHARMETANT, P.; LEROY, T. Chemical composition in wild ethiopian Arabica coffee accessions. Euphytica, v. 209, n. 2 p. 429-438, 2016.| Biblioteca(s): Embrapa Café. |
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| 28. | | SCHOLZ, M. B. S.; BORDIGNON, J. R.; FRANCO, F. A.; MARCHIORO, V. S.; EL KHOURI, M.; DE PIERI, F. L. Z.; MIRANDA, M. Z. de. Capacidade de retenção de solventes em farinha de trigo determinada por espectroscopia no infravermelho próximo. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 8.; SEMINÁRIO TÉCNICO DO TRIGO, 9., 2014, Canela; REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 9.; SEMINÁRIO TÉCNICO DO TRIGO, 10., 2015, Passo Fundo. Anais... Passo Fundo: Biotrigo Genética: Embrapa Trigo, 2015. 2014 - Melhoramento, Aptidão industrial e Sementes - Trabalho 88. 1 CD-ROM.| Biblioteca(s): Embrapa Trigo. |
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| 29. | | RIEDE, C. R.; CAMPOS, L. A. C.; SCHOLZ, M. B. dos S.; SHIOGA, P. S.; POLA, J. N.; OKUYAMA, L. A. IPR 109 - Bread wheat cultivar. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 4, n. 2, p. 244-247, June 2004. Cultivar release.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 30. | | RIEDE, C. R.; CAMPOS, L. A. C.; GARBUGLIO, D. D.; OKUYAMA, L. A.; SCHOLZ, M. B. dos; SERA, G. H.; SHIOGA, P. S.; POLA, J. N. IPR Catuara TM - cultivar de trigo melhorador. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 5., 2011, Dourados. Ata e resumos... Dourados: Embrapa Agropecuária Oeste, 2011. 4 p. Melhoramento, Aptidão Industrial e Sementes. 1 CD-ROM.| Biblioteca(s): Embrapa Trigo. |
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| 31. | | RIEDE, C. R.; CAMPOS, L. A. C.; MACHADO, J. C.; OKUYAMA, L. A.; SCHOLZ, M. B. dos S.; SHIOGA, P. S.; POLA, J. N. IPR Catuara TM - cultivar de trigo melhorador. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 6., 2011, Búzios. Panorama atual e perspectivas do melhoramento de plantas no Brasil. [Búzios]: SBMP, 2011. 3 p.| Biblioteca(s): Embrapa Trigo. |
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| 32. | | RIEDE, C. R.; CAMPOS, L. A. C.; MACHADO, J. C.; OKUYAMA, L. A.; SCHOLZ, M. B. DOS S.; SHIOGA, P. S.; POLA, J. N. IPR catuara TM - cultivar de trigo melhorador. In: Congresso Brasileiro de Melhoramento de Plantas, 6., 2011, Búzios. Anais...| Biblioteca(s): Embrapa Gado de Leite. |
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| 33. | | POT, D.; SCHOLZ, M. B. dos S.; LANNES, S. D.; DEL GROSSI, L.; PEREIRA, L. F. P.; VIEIRA, L. G.; SERA, T. Phenotypic analysis of coffea arabica accessions from ethipia: contribution to the understanding of coffea arabica diversity. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas.| Biblioteca(s): Embrapa Café. |
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| 34. | | PAGIATTO, N. F.; IVAMOTO, S. T.; SERA, T.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; CHARMETANT, P.; LEROY, T.; DOMINGUES, D.; PEREIRA, L. F. P. Evaluation of kahweol and cafestol levels in Ethiopian Coffea arabica L. accessions grown in Brazil. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 217| Biblioteca(s): Embrapa Café. |
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| 35. | | SANT'ANA, G. C.; POT, D.; IVAMOTO, S. T.; DOMINGUES, D. S.; SCHOLZ, M. B. B.; LEROY, T.; PEREIRA, L. F. P. Genomic regions associated to lipids and diterpenes contents slected during domestication and breeding of Coffea arabica. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro. Maringá, PR: SBMP, 2017. p. 72| Biblioteca(s): Embrapa Café. |
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| 36. | | IVAMOTO, S. T.; CALEDÓN, J. M.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; BOHLMANN, J.; DOMINGUES, D. S.; PEREIRA, L. F. P. Transcriptome analysis and characterization of genes involved in diterpene biosynthesis in coffea arabica. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro. Maringá, PR: SBMP, 2017. p. 105| Biblioteca(s): Embrapa Café. |
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| 37. | | SCHOLZ, M. B. S.; PAGIATTO, N. F.; KITZBERGER, C. S. G.; PEREIRA, L. F. P.; DAVRIEUX, F.; CHARMETANTE, P.; LEROYE, T. Validation of near-infrared spectroscopy for the quantification of cafestol and kahweol in green coffee. Food Research International, v. 61, p. 176-182, 2014.| Biblioteca(s): Embrapa Café. |
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| 38. | | RAKOCEVIC, M.; MALAGODI-BRAGA, K. S.; BATISTA, E. R.; MAIA, A. de H. N.; SCHOLZ, M. B. S.; FILIZOLA, H. F. The vegetative growth assists to reproductive responses of Arabic coffee trees in a long-term FACE experiment. Plant Growth Regulation, v. 91, n. 2, p. 305?316, 2020.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 39. | | GEROMEL, C.; FERREIRA, L. P.; DAVRIEUX, F.; GUYOT, B.; RIBEYRE, F.; SCHOLZ, M. B. dos S.; PEREIRA, L. F. P.; VAAST, P.; POT, D.; LEROY, T.; ANDROCIOLI FILHO, A.; VIEIRA, L. G. E.; MAZZAFERA, P.; MARRACCINI, P. Effects of shade on the development and sugar metabolism of coffee (Coffea arabica L.) fruits. PLANT PHYSIOLOGY AND BIOCHEMISTRY, v.46, n.5-6, p.569-579, may-june, 2008. 569-579| Biblioteca(s): Embrapa Café; Embrapa Unidades Centrais. |
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| 40. | | MARRACCINI, P.; GEROMEL, C.; FERREIRA, L. P.; PEREIRA, L. F. P.; LEROY, T.; POT, D.; DAVRIEUX, F.; RIBEYRE, F.; GUYOT, B.; SCHOLZ, M. B. S.; ANDROCIOLI FILHO, A.; VIEIRA, L. G. E.; MAZZAFERA, P. Soluble sugars, enzymatic activities and gene expression during development of coffee fruits submitted to shade condition. In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 57 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
21/12/2017 |
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Data da última atualização: |
21/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
IVOMOTO, S. T.; SAKURAY, L. M.; FERREIRA, L. P.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; POT, D.; LEROY, T.; VIEIRA, L. G. E.; DOMINGUES, D. S.; PEREIRA, L. F. P. |
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Afiliação: |
Suzana T. Ivamoto, IAPAR/UEL; Leonardo M. Sakuray, IAPAR/UEL; Lucia P. Ferreira, IAPAR; Cíntia S. G. Kitzberger, IAPAR; Maria B. S. Scholz, IAPAR; David Pot, CIRAD; Thierry Leroy, CIRAD; Luiz G. E. Vieira, UNOEST; Douglas S. Domingues, UNESP; LUIZ FILIPE PROTASIO PEREIRA, SAPC. |
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Título: |
Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Plant Physiology and Biochemistry, v. 111, p. 340-347, February, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Lipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30e240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways. MenosLipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30e240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warra... Mostrar Tudo |
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Palavras-Chave: |
Cafestol; Kahweol; RT-qPCR. |
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Thesagro: |
Café. |
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Thesaurus NAL: |
Gene expression; High performance liquid chromatography. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169675/1/Diterpenes-biochemical-profile.pdf
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Marc: |
LEADER 02581naa a2200301 a 4500
001 2083355
005 2017-12-21
008 2017 bl uuuu u00u1 u #d
100 1 $aIVOMOTO, S. T.
245 $aDiterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development.$h[electronic resource]
260 $c2017
520 $aLipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30e240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways.
650 $aGene expression
650 $aHigh performance liquid chromatography
650 $aCafé
653 $aCafestol
653 $aKahweol
653 $aRT-qPCR
700 1 $aSAKURAY, L. M.
700 1 $aFERREIRA, L. P.
700 1 $aKITZBERGER, C. S. G.
700 1 $aSCHOLZ, M. B. S.
700 1 $aPOT, D.
700 1 $aLEROY, T.
700 1 $aVIEIRA, L. G. E.
700 1 $aDOMINGUES, D. S.
700 1 $aPEREIRA, L. F. P.
773 $tPlant Physiology and Biochemistry$gv. 111, p. 340-347, February, 2017.
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