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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/11/2020 |
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Data da última atualização: |
23/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DINATO, N. B.; SANTOS, I. R. I.; VIGNA, B. B. Z.; PAULA, A. F. de; FAVERO, A. P. |
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Afiliação: |
Naiana Barbosa Dinato, UFSCAR; IZULME RITA IMACULADA SANTOS, Cenargen; BIANCA BACCILI ZANOTTO VIGNA, CPPSE; Ailton Ferreira de Paula, UFSCAR; ALESSANDRA PEREIRA FAVERO, CPPSE. |
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Título: |
Pollen cryopreservation for plant breeding and genetic resources conservation. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Cryoletters, v. 41, n. 3, p. 115-127, may 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Pollen conservation is an important tool for the maintenance of plant genetic resources and can promote improved efficiency in breeding programs and germplasm conservation and exchange. This review aims to highlight the importance of pollen cryopreservation and how to use it for distinct species in order to encourage the use of this methodology in germplasm banks and plant breeding programs. Pollen from many plant species have already been successfully cryopreserved in liquid nitrogen. Analogous with other plant structures, to maintain pollen viability after storage at ultra-low temperatures it is necessary to adjust the water content so that at least the freezable is removed. Optimum pollen moisture levels for cryopreservation varies among species and different methods have been applied to control moisture content. Common methods to decrease pollen moisture content include exposure to saturated solutions of various salts (which have a well-defined relative humidity), silica gel, dry air or treatment with vitrification solutions. It is our understanding that pollen cryopreservation is a safe and practical alternative for conserving genetic material that is often neglected by potential users. The technique has the potential to overcome challenges of breeding programs, such as flowering asynchrony between different parent genotypes, and the production of insufficient pollen in nature. Generally, pollen cryopreservation techniques tend to be simple enough to be used routinely in research, plant breeding and germplasm conservation programs. MenosPollen conservation is an important tool for the maintenance of plant genetic resources and can promote improved efficiency in breeding programs and germplasm conservation and exchange. This review aims to highlight the importance of pollen cryopreservation and how to use it for distinct species in order to encourage the use of this methodology in germplasm banks and plant breeding programs. Pollen from many plant species have already been successfully cryopreserved in liquid nitrogen. Analogous with other plant structures, to maintain pollen viability after storage at ultra-low temperatures it is necessary to adjust the water content so that at least the freezable is removed. Optimum pollen moisture levels for cryopreservation varies among species and different methods have been applied to control moisture content. Common methods to decrease pollen moisture content include exposure to saturated solutions of various salts (which have a well-defined relative humidity), silica gel, dry air or treatment with vitrification solutions. It is our understanding that pollen cryopreservation is a safe and practical alternative for conserving genetic material that is often neglected by potential users. The technique has the potential to overcome challenges of breeding programs, such as flowering asynchrony between different parent genotypes, and the production of insufficient pollen in nature. Generally, pollen cryopreservation techniques tend to be simple enough to be used routinely i... Mostrar Tudo |
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Palavras-Chave: |
Liquid nitrogen; Plant cryopreservation; Pollen grain. |
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Thesaurus Nal: |
Germplasm conservation; Hybridization. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/217991/1/s1.pdf
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Marc: |
LEADER 02230naa a2200229 a 4500
001 2126790
005 2020-11-23
008 2020 bl uuuu u00u1 u #d
100 1 $aDINATO, N. B.
245 $aPollen cryopreservation for plant breeding and genetic resources conservation.$h[electronic resource]
260 $c2020
520 $aPollen conservation is an important tool for the maintenance of plant genetic resources and can promote improved efficiency in breeding programs and germplasm conservation and exchange. This review aims to highlight the importance of pollen cryopreservation and how to use it for distinct species in order to encourage the use of this methodology in germplasm banks and plant breeding programs. Pollen from many plant species have already been successfully cryopreserved in liquid nitrogen. Analogous with other plant structures, to maintain pollen viability after storage at ultra-low temperatures it is necessary to adjust the water content so that at least the freezable is removed. Optimum pollen moisture levels for cryopreservation varies among species and different methods have been applied to control moisture content. Common methods to decrease pollen moisture content include exposure to saturated solutions of various salts (which have a well-defined relative humidity), silica gel, dry air or treatment with vitrification solutions. It is our understanding that pollen cryopreservation is a safe and practical alternative for conserving genetic material that is often neglected by potential users. The technique has the potential to overcome challenges of breeding programs, such as flowering asynchrony between different parent genotypes, and the production of insufficient pollen in nature. Generally, pollen cryopreservation techniques tend to be simple enough to be used routinely in research, plant breeding and germplasm conservation programs.
650 $aGermplasm conservation
650 $aHybridization
653 $aLiquid nitrogen
653 $aPlant cryopreservation
653 $aPollen grain
700 1 $aSANTOS, I. R. I.
700 1 $aVIGNA, B. B. Z.
700 1 $aPAULA, A. F. de
700 1 $aFAVERO, A. P.
773 $tCryoletters$gv. 41, n. 3, p. 115-127, may 2020.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
03/10/2012 |
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Data da última atualização: |
19/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MELO, J. O.; LANA, U. G. de P.; PIÑEROS, M. A.; ALVES, V. M. C.; GUIMARAES, C. T.; LIU, J.; ZHENG, Y.; ZHONG, S.; FEI, Z.; MARON, L. G.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. de. |
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Afiliação: |
UBIRACI GOMES DE PAULA LANA, CNPMS; VERA MARIA CARVALHO ALVES, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; ROBERT EUGENE SCHAFFERT, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS. |
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Título: |
Incomplete transfer of accessory loci influencing SbMATE expression underlies genetic background effects for aluminum tolerance in sorghum. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
The Plant Journal, Oxford, v. 73, p. 276-288, Jan. 2013. |
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DOI: |
10.1111/tpj.12029 |
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Idioma: |
Inglês |
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Conteúdo: |
Impaired root development caused by aluminum (Al) toxicity is a major cause of grain yield reduction in crops cultivated on acid soils, which are widespread worldwide. In sorghum, the major Al-tolerance locus, AltSB, is due to the function of SbMATE, which is an Al-activated root citrate transporter. Here we performed a molecular and physiological characterization of various AltSB donors and near-isogenic lines harboring various AltSB alleles. We observed a partial transfer of Al tolerance from the parents to the nearisogenic lines that was consistent across donor alleles, emphasizing the occurrence of strong genetic back-ground effects related to AltSB. This reduction in tolerance was variable, with a 20% reduction being observed when highly Al-tolerant lines were the AltSB donors, and a reduction as great as 70% when other AltSB alleles were introgressed. This reduction in Al tolerance was closely correlated with a reduction in SbMATE expression in near-isogenic lines, suggesting incomplete transfer of loci acting in trans on SbMATE. Nevertheless, Alt SB alleles from the highly Al-tolerant sources SC283 and SC566 were found to retain high SbMATE expression, presumably via elements present within or near the AltSB locus, resulting in significant transfer of the Al-tolerance phenotype to the derived near-isogenic lines. Allelic effects could not be explained by coding region polymorphisms, although occasional mutations may affect Al tolerance. Finally, we report on the extensive occurrence of alternative splicing for SbMATE, which may be an important component regulating SbMATE expression in sorghum by means of the nonsense-mediated RNA decay pathway. MenosImpaired root development caused by aluminum (Al) toxicity is a major cause of grain yield reduction in crops cultivated on acid soils, which are widespread worldwide. In sorghum, the major Al-tolerance locus, AltSB, is due to the function of SbMATE, which is an Al-activated root citrate transporter. Here we performed a molecular and physiological characterization of various AltSB donors and near-isogenic lines harboring various AltSB alleles. We observed a partial transfer of Al tolerance from the parents to the nearisogenic lines that was consistent across donor alleles, emphasizing the occurrence of strong genetic back-ground effects related to AltSB. This reduction in tolerance was variable, with a 20% reduction being observed when highly Al-tolerant lines were the AltSB donors, and a reduction as great as 70% when other AltSB alleles were introgressed. This reduction in Al tolerance was closely correlated with a reduction in SbMATE expression in near-isogenic lines, suggesting incomplete transfer of loci acting in trans on SbMATE. Nevertheless, Alt SB alleles from the highly Al-tolerant sources SC283 and SC566 were found to retain high SbMATE expression, presumably via elements present within or near the AltSB locus, resulting in significant transfer of the Al-tolerance phenotype to the derived near-isogenic lines. Allelic effects could not be explained by coding region polymorphisms, although occasional mutations may affect Al tolerance. Finally, we report on the exten... Mostrar Tudo |
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Palavras-Chave: |
Aluminum tolerance; Multidrug and toxic compound extrusion family; Tolerância ao alumínio; Transporter protein. |
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Thesagro: |
Gene; Sorghum bicolor. |
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Thesaurus NAL: |
alternative splicing. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02782naa a2200361 a 4500
001 1935385
005 2017-05-19
008 2013 bl uuuu u00u1 u #d
024 7 $a10.1111/tpj.12029$2DOI
100 1 $aMELO, J. O.
245 $aIncomplete transfer of accessory loci influencing SbMATE expression underlies genetic background effects for aluminum tolerance in sorghum.$h[electronic resource]
260 $c2013
520 $aImpaired root development caused by aluminum (Al) toxicity is a major cause of grain yield reduction in crops cultivated on acid soils, which are widespread worldwide. In sorghum, the major Al-tolerance locus, AltSB, is due to the function of SbMATE, which is an Al-activated root citrate transporter. Here we performed a molecular and physiological characterization of various AltSB donors and near-isogenic lines harboring various AltSB alleles. We observed a partial transfer of Al tolerance from the parents to the nearisogenic lines that was consistent across donor alleles, emphasizing the occurrence of strong genetic back-ground effects related to AltSB. This reduction in tolerance was variable, with a 20% reduction being observed when highly Al-tolerant lines were the AltSB donors, and a reduction as great as 70% when other AltSB alleles were introgressed. This reduction in Al tolerance was closely correlated with a reduction in SbMATE expression in near-isogenic lines, suggesting incomplete transfer of loci acting in trans on SbMATE. Nevertheless, Alt SB alleles from the highly Al-tolerant sources SC283 and SC566 were found to retain high SbMATE expression, presumably via elements present within or near the AltSB locus, resulting in significant transfer of the Al-tolerance phenotype to the derived near-isogenic lines. Allelic effects could not be explained by coding region polymorphisms, although occasional mutations may affect Al tolerance. Finally, we report on the extensive occurrence of alternative splicing for SbMATE, which may be an important component regulating SbMATE expression in sorghum by means of the nonsense-mediated RNA decay pathway.
650 $aalternative splicing
650 $aGene
650 $aSorghum bicolor
653 $aAluminum tolerance
653 $aMultidrug and toxic compound extrusion family
653 $aTolerância ao alumínio
653 $aTransporter protein
700 1 $aLANA, U. G. de P.
700 1 $aPIÑEROS, M. A.
700 1 $aALVES, V. M. C.
700 1 $aGUIMARAES, C. T.
700 1 $aLIU, J.
700 1 $aZHENG, Y.
700 1 $aZHONG, S.
700 1 $aFEI, Z.
700 1 $aMARON, L. G.
700 1 $aSCHAFFERT, R. E.
700 1 $aKOCHIAN, L. V.
700 1 $aMAGALHAES, J. V. de
773 $tThe Plant Journal, Oxford$gv. 73, p. 276-288, Jan. 2013.
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Embrapa Milho e Sorgo (CNPMS) |
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