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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
05/03/2012 |
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Data da última atualização: |
19/01/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
WIEBKE-STROHM, B.; PASQUALI, G.; MARGIS-PINHEIRO, M.; BENCKE, M.; BÜCKER-NETO, L.; BECKER-RITT, A. B.; MARTINELLI, A. H. S.; RECHENMACHER, C.; POLACCO, J. C.; STOLF, R.; MARCELINO, F. C.; ABDELNOOR, R. V.; HOMRICH, M. S.; DEL PONTE, E. M.; CARLINI, C. R.; CARVALHO, M. C. C. G. de; BODANESE-ZANETTINI, M. H. |
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Afiliação: |
BEATRIZ WIEBKE-STROHM, UFRGS; GIANCARLO PASQUALI, UFRGS; MÁRCIA MARGIS-PINHEIRO, UFRGS; MARTA BENCKE, UFRGS; LAURO BÜCKER NETO, UFRGS; ARLETE B. BECKER-RITT, UFRGS; ANNE H. S. MARTINELLI, UFRGS; CILIANA RECHENMACHER, UFRGS; JOSEPH C. POLACCO, University of Missouri; RENATA STOLF, CNPSo; FRANCISMAR CORREA MARCELINO, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; MILENA S. HOMRICH, UFRGS; EMERSON M. DEL PONTE, UFRGS; CELIA R. CARLINI, UFRGS; MAYRA C. C. G. DE CARVALHO, CNPSo; MARIA HELENA BODANESE-ZANETTINI, UFRGS. |
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Título: |
Ubiquitous urease affects soybean susceptibility to fungi. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Plant Molecular Biology, The Hague, v. 79, n. 1/2, p. 75-87, May 2012. |
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DOI: |
DOI 10.1007/s11103-012-9894-1 |
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Idioma: |
Inglês |
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Conteúdo: |
The soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach. MenosThe soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of u... Mostrar Tudo |
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Palavras-Chave: |
Penicillium herguei; Transformação genética. |
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Thesagro: |
Fungo; Phomopsis; Queima da saia; Soja. |
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Thesaurus Nal: |
Genetic transformation; Soybeans. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02843naa a2200421 a 4500
001 1917463
005 2017-01-19
008 2012 bl uuuu u00u1 u #d
024 7 $aDOI 10.1007/s11103-012-9894-1$2DOI
100 1 $aWIEBKE-STROHM, B.
245 $aUbiquitous urease affects soybean susceptibility to fungi.
260 $c2012
520 $aThe soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach.
650 $aGenetic transformation
650 $aSoybeans
650 $aFungo
650 $aPhomopsis
650 $aQueima da saia
650 $aSoja
653 $aPenicillium herguei
653 $aTransformação genética
700 1 $aPASQUALI, G.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aBENCKE, M.
700 1 $aBÜCKER-NETO, L.
700 1 $aBECKER-RITT, A. B.
700 1 $aMARTINELLI, A. H. S.
700 1 $aRECHENMACHER, C.
700 1 $aPOLACCO, J. C.
700 1 $aSTOLF, R.
700 1 $aMARCELINO, F. C.
700 1 $aABDELNOOR, R. V.
700 1 $aHOMRICH, M. S.
700 1 $aDEL PONTE, E. M.
700 1 $aCARLINI, C. R.
700 1 $aCARVALHO, M. C. C. G. de
700 1 $aBODANESE-ZANETTINI, M. H.
773 $tPlant Molecular Biology, The Hague$gv. 79, n. 1/2, p. 75-87, May 2012.
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Registro original: |
Embrapa Soja (CNPSO) |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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URL |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
14/09/2000 |
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Data da última atualização: |
13/04/2018 |
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Tipo da produção científica: |
Autoria/Organização/Edição de Livros |
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Autoria: |
SCHAEFER, C. W.; PANIZZI, A. R. |
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Afiliação: |
CARL W. SCHAEFER, University of Connecticut; ANTONIO RICARDO PANIZZI, CNPSo. |
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Título: |
Heteroptera of economic importance. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Boca Raton: CRC, 2000. |
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Páginas: |
828p. |
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ISBN: |
0-8493-0695-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Economic importance of heteroptera: a general view; Possible causes of disease symptoms resulting from the feeding of phytophagous heteroptera; Plant bugs (Miridae) as plant pests; Lace bugs (Tingidae); Palm bugs (Thaumastocoridae); Seed and chinch bugs (Lygaeoidea); Ash-gray leaf bugs (Piesmatidae); Cotton stainers and their relatives (Pyrrhocoroidea: Pyrhocoridae and Largidae); Scentless plant bugs (Rhopalidae); Broad-headed bugs (Alydidae); Leaf-footed bugs (Coreidae); Burrower bugs (Cydnidae); Stink bugs (Pentatomidae); Shield bugs (Scutelleridae); Several small pentatomoid families (Cyrtocoridae, Dinidoridae, Eurostylidae, Plataspidae, and Tessaratomidae); Flat bugs (Aradidae); Bed bugs (Cimicidae); Triatominae (Reduviidae); Adventitious biters - "Nuisance" bugs; How carnivorous bugs feed; Creeping water bugs (Naucoridae); Giant water bugs (Belostomatidae); Waterscorpions (Nepidae); Small aquatic bugs (Nepomorpha) with slight or underestimated economic importance; Semiaquatic bugs (Gerromorpha); Minute pirate bugs (Anthocoridae); Damsel bugs (Nabidae); Predacious plant bugs (Miridae); Assassin bugs (Reduviidae excluding Triatominae); Economic importance of predation by Big-Eyed bugs (Geocoridae); Stilt bugs (Berytidae); Predaceous stinkbugs (Pentatomidae: Asopinae). |
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Palavras-Chave: |
Inseto praga; Inseto util; Pest insects; Useful insects. |
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Thesagro: |
Hemíptera. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01779nam a2200205 a 4500
001 1462248
005 2018-04-13
008 2000 bl uuuu 00u1 u #d
020 $a0-8493-0695-7
100 1 $aSCHAEFER, C. W.
245 $aHeteroptera of economic importance.
260 $aBoca Raton: CRC$c2000
300 $a828p.
520 $aEconomic importance of heteroptera: a general view; Possible causes of disease symptoms resulting from the feeding of phytophagous heteroptera; Plant bugs (Miridae) as plant pests; Lace bugs (Tingidae); Palm bugs (Thaumastocoridae); Seed and chinch bugs (Lygaeoidea); Ash-gray leaf bugs (Piesmatidae); Cotton stainers and their relatives (Pyrrhocoroidea: Pyrhocoridae and Largidae); Scentless plant bugs (Rhopalidae); Broad-headed bugs (Alydidae); Leaf-footed bugs (Coreidae); Burrower bugs (Cydnidae); Stink bugs (Pentatomidae); Shield bugs (Scutelleridae); Several small pentatomoid families (Cyrtocoridae, Dinidoridae, Eurostylidae, Plataspidae, and Tessaratomidae); Flat bugs (Aradidae); Bed bugs (Cimicidae); Triatominae (Reduviidae); Adventitious biters - "Nuisance" bugs; How carnivorous bugs feed; Creeping water bugs (Naucoridae); Giant water bugs (Belostomatidae); Waterscorpions (Nepidae); Small aquatic bugs (Nepomorpha) with slight or underestimated economic importance; Semiaquatic bugs (Gerromorpha); Minute pirate bugs (Anthocoridae); Damsel bugs (Nabidae); Predacious plant bugs (Miridae); Assassin bugs (Reduviidae excluding Triatominae); Economic importance of predation by Big-Eyed bugs (Geocoridae); Stilt bugs (Berytidae); Predaceous stinkbugs (Pentatomidae: Asopinae).
650 $aHemíptera
653 $aInseto praga
653 $aInseto util
653 $aPest insects
653 $aUseful insects
700 1 $aPANIZZI, A. R.
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