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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
11/09/2018 |
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Data da última atualização: |
31/10/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KISHI, R. N. I.; STACH-MACHADO, D.; SINGULANI, J. de L.; SANTOS, C. T. dos; FUSCO-ALMEIDA, A. M.; CILLI, E. M.; ASTUA, J. de F.; PICCHI, S. C.; MACHADO, M. A. |
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Afiliação: |
ROSANGELA NAOMI INUI KISHI, Instituto Agronomico; DAGMAR STACH-MACHADO, Universidade Estadual de Campinas; JUNYA DE LACORTE SINGULANI, Universidade Estadual Paulista Julio de Mesquita Filho; CLAUDIA TAVARES DOS SANTOS, Universidade Estadual Paulista Julio de Mesquita Filho; ANA MARISA FUSCO-ALMEIDA, Universidade Estadual Paulista Julio de Mesquita Filho; EDUARDO MAFFUD CILLI, Universidade Estadual Paulista Julio de Mesquita Fil; JULIANA DE FREITAS ASTUA, CNPMF; SIMONE CRISTINA PICCHI, Instituto Agronomico; MARCOS ANTONIO MACHADO, Instituto Agronômico. |
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Título: |
Evaluation of cytotoxicity features of antimicrobial peptides with potential to control bacterial diseases of citrus. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Plos One, 2018. |
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ISSN: |
1932-6203 |
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DOI: |
10.1371/journal. pone.0203451 |
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Idioma: |
Inglês |
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Conteúdo: |
considered an alternative for pesticides used to control plant pathogens, including those affecting citrus. Brazil is the largest producer and exporter of frozen concentrated orange juice in the world. However, the citrus industry has been affected by several diseases such as citrus canker and huanglongbing (HLB), caused by the bacteria Xanthomonas citri subsp. citri (X.citri) and Candidatus Liberibacter asiaticus (CaLas), respectively. In order to control these pathogens, putative AMPs were prospected in databases containing citrus sequences. Furthermore, AMPs already reported in the literature were also used for in vitro and in vivo assays against X.citri. Since CaLas cannot be cultivated in vitro, surrogates as Sinorhizobium meliloti and Agrobacterium tumefaciens were used. This study reports the evaluation of six AMPs obtained from different sources, two of them from Citrus spp. (citrus-amp1 and citrus-amp2), three from amphibians (Hylin-a1, K0-W6-Hy-a1 and Ocellatin 4-analogue) and one from porcine (Tritrpticin). Peptides K0-W6-Hy-a1, Ocellatin 4-analogue, and citrus-amp1 showed bactericidal activity against X.citri and S. meliloti and bacteriostatic effect on A. tumefaciens. These results were confirmed for X.citri in planta. In addition cytotoxicity evaluations of these molecules were performed. The AMPs that showed the lowest hemolytic activities were Triptrpticin, citrus-amp1 and citrus-amp2. Citrus-amp1 and citrus-amp2 not presented toxicity in experiments using in vivo model, G. mellonella and U87 MG cells. To verify the interaction of these AMPs with bacteria and erythrocyte cell membranes, vesicles mimicking these cells were built. Citrus-amp1 and Tritrpticin exhibited higher affinity to bacterial membranes, while Ocellatin 4-analogue and Hylin-a1 showed higher affinity to erythrocyte membranes; exclude their use in citrus. This work demonstrates an essential alternative, trough AMPs obtained from Citrus spp., which can be feasibly used to control bacterial pathogens. Menosconsidered an alternative for pesticides used to control plant pathogens, including those affecting citrus. Brazil is the largest producer and exporter of frozen concentrated orange juice in the world. However, the citrus industry has been affected by several diseases such as citrus canker and huanglongbing (HLB), caused by the bacteria Xanthomonas citri subsp. citri (X.citri) and Candidatus Liberibacter asiaticus (CaLas), respectively. In order to control these pathogens, putative AMPs were prospected in databases containing citrus sequences. Furthermore, AMPs already reported in the literature were also used for in vitro and in vivo assays against X.citri. Since CaLas cannot be cultivated in vitro, surrogates as Sinorhizobium meliloti and Agrobacterium tumefaciens were used. This study reports the evaluation of six AMPs obtained from different sources, two of them from Citrus spp. (citrus-amp1 and citrus-amp2), three from amphibians (Hylin-a1, K0-W6-Hy-a1 and Ocellatin 4-analogue) and one from porcine (Tritrpticin). Peptides K0-W6-Hy-a1, Ocellatin 4-analogue, and citrus-amp1 showed bactericidal activity against X.citri and S. meliloti and bacteriostatic effect on A. tumefaciens. These results were confirmed for X.citri in planta. In addition cytotoxicity evaluations of these molecules were performed. The AMPs that showed the lowest hemolytic activities were Triptrpticin, citrus-amp1 and citrus-amp2. Citrus-amp1 and citrus-amp2 not presented toxicity in experiments using in... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Fruta Cítrica. |
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Thesaurus Nal: |
Citrus; Plant diseases and disorders; Replant disease. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182724/1/Inui-Kishi-et-al-2018-journal.pone.0203451.pdf
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Marc: |
LEADER 02901naa a2200301 a 4500
001 2095442
005 2018-10-31
008 2018 bl uuuu u00u1 u #d
022 $a1932-6203
024 7 $a10.1371/journal. pone.0203451$2DOI
100 1 $aKISHI, R. N. I.
245 $aEvaluation of cytotoxicity features of antimicrobial peptides with potential to control bacterial diseases of citrus.$h[electronic resource]
260 $c2018
520 $aconsidered an alternative for pesticides used to control plant pathogens, including those affecting citrus. Brazil is the largest producer and exporter of frozen concentrated orange juice in the world. However, the citrus industry has been affected by several diseases such as citrus canker and huanglongbing (HLB), caused by the bacteria Xanthomonas citri subsp. citri (X.citri) and Candidatus Liberibacter asiaticus (CaLas), respectively. In order to control these pathogens, putative AMPs were prospected in databases containing citrus sequences. Furthermore, AMPs already reported in the literature were also used for in vitro and in vivo assays against X.citri. Since CaLas cannot be cultivated in vitro, surrogates as Sinorhizobium meliloti and Agrobacterium tumefaciens were used. This study reports the evaluation of six AMPs obtained from different sources, two of them from Citrus spp. (citrus-amp1 and citrus-amp2), three from amphibians (Hylin-a1, K0-W6-Hy-a1 and Ocellatin 4-analogue) and one from porcine (Tritrpticin). Peptides K0-W6-Hy-a1, Ocellatin 4-analogue, and citrus-amp1 showed bactericidal activity against X.citri and S. meliloti and bacteriostatic effect on A. tumefaciens. These results were confirmed for X.citri in planta. In addition cytotoxicity evaluations of these molecules were performed. The AMPs that showed the lowest hemolytic activities were Triptrpticin, citrus-amp1 and citrus-amp2. Citrus-amp1 and citrus-amp2 not presented toxicity in experiments using in vivo model, G. mellonella and U87 MG cells. To verify the interaction of these AMPs with bacteria and erythrocyte cell membranes, vesicles mimicking these cells were built. Citrus-amp1 and Tritrpticin exhibited higher affinity to bacterial membranes, while Ocellatin 4-analogue and Hylin-a1 showed higher affinity to erythrocyte membranes; exclude their use in citrus. This work demonstrates an essential alternative, trough AMPs obtained from Citrus spp., which can be feasibly used to control bacterial pathogens.
650 $aCitrus
650 $aPlant diseases and disorders
650 $aReplant disease
650 $aDoença de Planta
650 $aFruta Cítrica
700 1 $aSTACH-MACHADO, D.
700 1 $aSINGULANI, J. de L.
700 1 $aSANTOS, C. T. dos
700 1 $aFUSCO-ALMEIDA, A. M.
700 1 $aCILLI, E. M.
700 1 $aASTUA, J. de F.
700 1 $aPICCHI, S. C.
700 1 $aMACHADO, M. A.
773 $tPlos One, 2018.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
24/02/2015 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CORDEIRO-ARAÚJO, M. K.; CHIA, M. A.; HEREMAN, T. C.; SASAKI, F. F. C.; BITTENCOURT-OLIVEIRA, M. do C. |
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Afiliação: |
MICHELINE KÉZIA CORDEIRO-ARAÚJO, ESALQ; MATHIAS AHII CHIA, ESALQ; TALITA CAROLINE HEREMAN, ESALQ; FABIANA FUMI CERQUEIRA SASAKI, CNPMF; MARIA DO CARMO BITTENCOURT-OLIVEIRA, ESALQ. |
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Título: |
Selective membrane permeability and peroxidase activity response of lettuce and arugula irrigated with cyanobacterialcontaminated water. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Environmental Earth Sciences, Februrary, 2015. |
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ISSN: |
1866-6299 |
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DOI: |
10.1007/s12665-015-4147-7 |
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Idioma: |
Português |
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Conteúdo: |
Irrigation with microcystins-contaminated water has been shown to cause oxidative stress and negatively affect the development of vegetables. However, the effect of non-microcystins producing cyanobacteria on vegetables is yet to be investigated. In this study, the effects of microcystin-producing (MC?) and non-microcystin-producing (MC-) cyanobacterial (Microcystis aeruginosa) extracts on lettuce (Lactuca sativa L.) and arugula (Erucasativa Mill.) were investigated. Chlorophyll production, peroxidase (POD) activity and selective membrane permeability of the vegetables were monitored after exposure to 0.6?12.5 lg L-1 MC? for 15 days. For MC- extracts, an equivalent biomass of each MC? extract concentration per total MCs concentration was also applied to the vegetables for 15 days. In arugula, exposure to both toxic and non-toxic cyanobacterial extracts resulted in higher POD activity than the control. However, in lettuce plants, significantly lower POD activities were recorded in the presence of MC? and MC- extracts. Although both crude (MC? and MC-) extracts increased plasma membrane electrical conductivity of the vegetables, the effect of MC? extract was higher. Chlorophyll content of both vegetables was not significantly influenced by MC? and MC- extracts. The results of the present study show that vegetables have variable responses to MC? and MC- extracts of M. aeruginosa. Therefore, care must be taken to avoid the excessive use of M. aeruginosa contaminated water to irrigate vegetables, regardless of their MCs production potential. MenosIrrigation with microcystins-contaminated water has been shown to cause oxidative stress and negatively affect the development of vegetables. However, the effect of non-microcystins producing cyanobacteria on vegetables is yet to be investigated. In this study, the effects of microcystin-producing (MC?) and non-microcystin-producing (MC-) cyanobacterial (Microcystis aeruginosa) extracts on lettuce (Lactuca sativa L.) and arugula (Erucasativa Mill.) were investigated. Chlorophyll production, peroxidase (POD) activity and selective membrane permeability of the vegetables were monitored after exposure to 0.6?12.5 lg L-1 MC? for 15 days. For MC- extracts, an equivalent biomass of each MC? extract concentration per total MCs concentration was also applied to the vegetables for 15 days. In arugula, exposure to both toxic and non-toxic cyanobacterial extracts resulted in higher POD activity than the control. However, in lettuce plants, significantly lower POD activities were recorded in the presence of MC? and MC- extracts. Although both crude (MC? and MC-) extracts increased plasma membrane electrical conductivity of the vegetables, the effect of MC? extract was higher. Chlorophyll content of both vegetables was not significantly influenced by MC? and MC- extracts. The results of the present study show that vegetables have variable responses to MC? and MC- extracts of M. aeruginosa. Therefore, care must be taken to avoid the excessive use of M. aeruginosa contaminated water to irr... Mostrar Tudo |
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Thesagro: |
Alface. |
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Thesaurus NAL: |
Cyanobacterial toxins; Enzyme activity; Vegetables. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02333naa a2200241 a 4500
001 2009644
005 2023-05-25
008 2015 bl uuuu u00u1 u #d
022 $a1866-6299
024 7 $a10.1007/s12665-015-4147-7$2DOI
100 1 $aCORDEIRO-ARAÚJO, M. K.
245 $aSelective membrane permeability and peroxidase activity response of lettuce and arugula irrigated with cyanobacterialcontaminated water.$h[electronic resource]
260 $c2015
520 $aIrrigation with microcystins-contaminated water has been shown to cause oxidative stress and negatively affect the development of vegetables. However, the effect of non-microcystins producing cyanobacteria on vegetables is yet to be investigated. In this study, the effects of microcystin-producing (MC?) and non-microcystin-producing (MC-) cyanobacterial (Microcystis aeruginosa) extracts on lettuce (Lactuca sativa L.) and arugula (Erucasativa Mill.) were investigated. Chlorophyll production, peroxidase (POD) activity and selective membrane permeability of the vegetables were monitored after exposure to 0.6?12.5 lg L-1 MC? for 15 days. For MC- extracts, an equivalent biomass of each MC? extract concentration per total MCs concentration was also applied to the vegetables for 15 days. In arugula, exposure to both toxic and non-toxic cyanobacterial extracts resulted in higher POD activity than the control. However, in lettuce plants, significantly lower POD activities were recorded in the presence of MC? and MC- extracts. Although both crude (MC? and MC-) extracts increased plasma membrane electrical conductivity of the vegetables, the effect of MC? extract was higher. Chlorophyll content of both vegetables was not significantly influenced by MC? and MC- extracts. The results of the present study show that vegetables have variable responses to MC? and MC- extracts of M. aeruginosa. Therefore, care must be taken to avoid the excessive use of M. aeruginosa contaminated water to irrigate vegetables, regardless of their MCs production potential.
650 $aCyanobacterial toxins
650 $aEnzyme activity
650 $aVegetables
650 $aAlface
700 1 $aCHIA, M. A.
700 1 $aHEREMAN, T. C.
700 1 $aSASAKI, F. F. C.
700 1 $aBITTENCOURT-OLIVEIRA, M. do C.
773 $tEnvironmental Earth Sciences, Februrary, 2015.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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