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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/02/2017 |
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Data da última atualização: |
11/10/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARENA, G. D.; RAMOS-GONZÁLEZ, P. L.; NUNES, M. A.; JESUS, C. C.; CALEGARIO, R. F.; KITAJIMA, E. W.; NOVELLI, V. M.; ASTUA, J. de F. |
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Afiliação: |
GABRIELLA DIAS ARENA; PEDRO LUIS RAMOS-GONZÁLEZ; MARIA ANDRÉIA NUNES; CAMILA CHABI JESUS; RENATA FAIER CALEGARIO; ELLIOT WATANABE KITAJIMA; VALDENICE MOREIRA NOVELLI; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Scientia Agricola, v.74, n.1, p.1-10X, 2017 |
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ISSN: |
1678-992X |
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DOI: |
10.1590/1678-992X-2015-0380 |
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Idioma: |
Inglês |
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Conteúdo: |
Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be subclassified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the ccumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV. Keywords: Clerodendrum chlorotic spot virus, Cilevirus, Solanum violaefolium ringspot virus, Coffee ringspot virus, Dichorhavirus MenosBrevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be subclassified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the ccumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV. Keywords: Clerodendrum chlorotic spot virus, Cilevirus, Solanum ... Mostrar Tudo |
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Thesagro: |
Doença de planta; Virus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02287naa a2200253 a 4500
001 2062765
005 2017-10-11
008 2017 bl uuuu u00u1 u #d
022 $a1678-992X
024 7 $a10.1590/1678-992X-2015-0380$2DOI
100 1 $aARENA, G. D.
245 $aArabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses.$h[electronic resource]
260 $c2017
520 $aBrevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be subclassified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the ccumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum chlorotic spot virus, and the BTV-C Solanum violaefolium ringspot virus, were mediated by viruliferous Brevipalpus mites collected in the wild. Upon infestation, local lesions appeared in 7 to 10 days on leaves of, at least, 80 % of the assayed plants. Presence of viral particles and characteristic cytopathic effects were detected by transmission electron microscopy (TEM) and the viral identities confirmed by specific reverse-transcriptase polymerase chain reaction (RT-PCR) and further amplicon sequencing. The high infection rate and reproducibility of symptoms of the three different viruses assayed validate A. thaliana as a feasible alternative experimental host for BTV. Keywords: Clerodendrum chlorotic spot virus, Cilevirus, Solanum violaefolium ringspot virus, Coffee ringspot virus, Dichorhavirus
650 $aDoença de planta
650 $aVirus
700 1 $aRAMOS-GONZÁLEZ, P. L.
700 1 $aNUNES, M. A.
700 1 $aJESUS, C. C.
700 1 $aCALEGARIO, R. F.
700 1 $aKITAJIMA, E. W.
700 1 $aNOVELLI, V. M.
700 1 $aASTUA, J. de F.
773 $tScientia Agricola$gv.74, n.1, p.1-10X, 2017
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
03/10/1997 |
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Data da última atualização: |
06/11/2012 |
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Autoria: |
SANTOS, T. M. dos; WAQUIL, J. M.; ANDRADE, R. V. de. |
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Afiliação: |
EMBRAPA/CNPMS. |
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Título: |
Avaliação de germoplasma de sorgo para resistência a Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae). |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
In: EMBRAPA. Centro de Nacional de Pesquisa de Milho e Sorgo. Relatório técnico anual do Centro Nacional de Pesquisa de Milho e Sorgo 1988-1991. Sete Lagoas, 1992. p. 98-100. |
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Idioma: |
Português |
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Palavras-Chave: |
Pest; Pragas; Resistance. |
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Thesagro: |
Germoplasma; Melhoramento; Resistência; Sorghum Bicolor; Sorgo; Spodoptera Frugiperda. |
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Thesaurus NAL: |
breeding; germplasm. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/69584/1/Avaliacao-germoplasma.pdf
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Marc: |
LEADER 00896naa a2200265 a 4500
001 1476813
005 2012-11-06
008 1992 bl uuuu u00u1 u #d
100 1 $aSANTOS, T. M. dos
245 $aAvaliação de germoplasma de sorgo para resistência a Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera$bNoctuidae).$h[electronic resource]
260 $c1992
650 $abreeding
650 $agermplasm
650 $aGermoplasma
650 $aMelhoramento
650 $aResistência
650 $aSorghum Bicolor
650 $aSorgo
650 $aSpodoptera Frugiperda
653 $aPest
653 $aPragas
653 $aResistance
700 1 $aWAQUIL, J. M.
700 1 $aANDRADE, R. V. de
773 $tIn: EMBRAPA. Centro de Nacional de Pesquisa de Milho e Sorgo. Relatório técnico anual do Centro Nacional de Pesquisa de Milho e Sorgo 1988-1991. Sete Lagoas, 1992. p. 98-100.
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