Data/hora: 25/06/2024 00:45:36

Biblioteca(s): | Embrapa Algodão.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 1
Data corrente: | 18/03/2016
Data da última atualização: | 18/03/2016
Autoria: | BATISTA, V. G. L.; PINHEIRO, M. P. N.; MELO FILHO, P. A.; SANTOS, R. C. dos; LIMA, L. M. de.
Afiliação: | VANDRE GUEVARA LYRA BATISTA, UEPB - CAMPINA GRANDE; MORGANA POLLYNNE NÓBREGA PINHEIRO, UFRPE - REDE NORDESTE DE BIOTECNOLOGIA; PÉRICLES DE ALBUQUERQUE MELO FILHO, UFRPE; ROSEANE CAVALCANTI DOS SANTOS, CNPA; LIZIANE MARIA DE LIMA, CNPA.
Título: | Temporal and tissue expression of genes involved in buds of earliness cotton cultivar.
Ano de publicação: | 2015
Fonte/Imprenta: | Genetics and Molecular Research, v. 14, n. 3, p. 7386-7394, 2015.
ISSN: | 1676-5680
Idioma: | Inglês
Conteúdo: | Gene sequences previously identified in Arabidopsis buds were used as references in order to estimate temporal and tissue expression in buds, leaves, stem, and root tissues in cotton plants. Buds were evaluated during 3 phases: 2-8, 10-12, and 14-20 mm. Primers were designed for the ARF6, ATFY, and SEUSS genes for use in semi-quantitative reverse transcription-polymerase chain reaction and quantitative reverse transcription-polymerase chain reaction. Different levels of expression of the 3 genes were confirmed in cotton buds as well as in other tissues. The peak of gene expression was observed in buds sized 10-12 mm, after which expression decreased in larger buds. The gene GhFYPP3 was the most promising for further prospection of promoter regions, with regular expression patterns observed in bud sizes 10-12 and 14-20 mm. This trait was not observed in others genes.
Palavras-Chave: | Bud development.
Thesagro: | Algodão; Botão floral; Gene; Gossypium hirsutum.
Thesaurus Nal: | cotton.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/141407/1/Temporal-and-tissue-expression-of-genes-....pdf
Registro original: | Embrapa Algodão (CNPA)
Exemplares: | CNPA - (28112-1) - UPC - AP - DD -  -  -  - DIS