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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
11/03/2014 |
Data da última atualização: |
12/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TIZIOTO, P.; GASPARINI, G.; SOUZA, M. M.; MUDADU, M. de A.; COUTINHO, L. L.; MOURÃO, G. B.; THOLON, P.; MEIRELLES, S. L. C.; TULLIO, R. R.; ROSA, A. do N.; ALENCAR, M. M. de; MEDEIROS, S. R.; SIQUEIRA, F.; FEIJO, G. L. D.; NASSU, R. T.; REGITANO, L. C. de A. |
Afiliação: |
POLIANA TIZIOTO, Universidade Federal de São Carlos; GUSTAVO GASPARINI, POS-GRADUAÇÃO UFSCar/SÃO CARLOS, SP; MARCELA M. SOUZA, POS-GRADUAÇÃO UFSCar/SÃO CARLOS, SP; MAURICIO DE ALVARENGA MUDADU, CPPSE; LUIZ L. COUTINHO, PROF. ESALQ/PIRACICABA; GERSON B. MOURÃO, ESALQ/PIRACICABA; PATRICIA THOLON, CPPSE; SARAH L. C. MEIRELLES, PROF. UFLA/LAVRAS; RYMER RAMIZ TULLIO, CPPSE; ANTONIO DO NASCIMENTO ROSA, CNPGC; MAURICIO MELLO DE ALENCAR, CPPSE; SERGIO R. MEDEIROS, CAMPO GRANDE; FABIANE SIQUEIRA, CNPGC; GELSON LUIS DIAS FEIJO, CNPGC; RENATA TIEKO NASSU, CPPSE; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
Título: |
Identification of KCNJ11 as a functional candidate gene for bovine meat tenderness. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Physiological Genomics, v. 45, n. 24, p. 1215-1221, 2013. |
DOI: |
10.1152/physiolgenomics.00137.2012 |
Idioma: |
Inglês |
Conteúdo: |
The potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to identify 22 SNPs. We validated two of the identified markers by genotyping the whole population (n = 460). Analysis of association between genotypes and WBSF values revealed a significant additive effect of a SNP at different meat aging times (P ? 0.05). In addition, an association between the expression levels of KCNJ11 and WBSF was found, with lower expression levels of KCNJ11 associated with more tender meat (P ? 0.05). The results showed that the KCNJ11 gene is a candidate mapped to a QTL for meat tenderness previously identified on BTA15 and may be useful to identify animals with genetic potential to produce tender meat. The effect of KCNJ11 observed on muscle is potentially due to changes in activity of KATP channels, which in turn influence the flow of potassium in the intracellular space, allowing establishment of the membrane potential necessary for muscle contraction. MenosThe potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to identify 22 SNPs. We validated two of the identified markers by genotyping the whole population (n = 460). Analysis of association between genotypes and WBSF values revealed a significant additive effect of a SNP at different meat aging times (P ? 0.05). In addition, an association between the expression levels of KCNJ11 and WBSF was found, with lower expression levels of KCNJ11 associated with more tender meat (P ? 0.05). The results showed that the KCNJ11 gene is a candidate mapped to a QTL for meat tenderness previously identified on BTA15 and may be useful to identify animals with genetic potential to produce tender meat. The effect of KCNJ11 observed on muscle is potentially due to changes in activity of KATP channels, whic... Mostrar Tudo |
Palavras-Chave: |
Bovine; Candidate gene; Differential expression; Molecular markers; Shear force; Warner Bratzler. |
Thesaurus Nal: |
beef; meat. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/108204/1/1215.full.pdf
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Marc: |
LEADER 02795naa a2200409 a 4500 001 1982035 005 2023-05-12 008 2013 bl uuuu u00u1 u #d 024 7 $a10.1152/physiolgenomics.00137.2012$2DOI 100 1 $aTIZIOTO, P. 245 $aIdentification of KCNJ11 as a functional candidate gene for bovine meat tenderness.$h[electronic resource] 260 $c2013 520 $aThe potassium inwardly rectifying channel, subfamily J, member 11 (KCNJ11) gene was investigated as a candidate for meat tenderness based on the effects reported on muscle for KCNJ11 gene knockout in rat models and its position in a quantitative trait locus (QTL) for meat tenderness in the bovine genome. Sequence variations in the KCNJ11 gene were described by sequencing six amplified fragments, covering almost the entire gene. We identified single nucleotide polymorphisms (SNP) and validated them by different approaches, taking advantage of simultaneous projects that are being developed with the same Nelore population. By sequencing the KCNJ11 in Nelore steers representing extreme phenotypes for Warner-Bratzler shear force (WBSF), it was possible to identify 22 SNPs. We validated two of the identified markers by genotyping the whole population (n = 460). Analysis of association between genotypes and WBSF values revealed a significant additive effect of a SNP at different meat aging times (P ? 0.05). In addition, an association between the expression levels of KCNJ11 and WBSF was found, with lower expression levels of KCNJ11 associated with more tender meat (P ? 0.05). The results showed that the KCNJ11 gene is a candidate mapped to a QTL for meat tenderness previously identified on BTA15 and may be useful to identify animals with genetic potential to produce tender meat. The effect of KCNJ11 observed on muscle is potentially due to changes in activity of KATP channels, which in turn influence the flow of potassium in the intracellular space, allowing establishment of the membrane potential necessary for muscle contraction. 650 $abeef 650 $ameat 653 $aBovine 653 $aCandidate gene 653 $aDifferential expression 653 $aMolecular markers 653 $aShear force 653 $aWarner Bratzler 700 1 $aGASPARINI, G. 700 1 $aSOUZA, M. M. 700 1 $aMUDADU, M. de A. 700 1 $aCOUTINHO, L. L. 700 1 $aMOURÃO, G. B. 700 1 $aTHOLON, P. 700 1 $aMEIRELLES, S. L. C. 700 1 $aTULLIO, R. R. 700 1 $aROSA, A. do N. 700 1 $aALENCAR, M. M. de 700 1 $aMEDEIROS, S. R. 700 1 $aSIQUEIRA, F. 700 1 $aFEIJO, G. L. D. 700 1 $aNASSU, R. T. 700 1 $aREGITANO, L. C. de A. 773 $tPhysiological Genomics$gv. 45, n. 24, p. 1215-1221, 2013.
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Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
02/02/2009 |
Data da última atualização: |
23/11/2022 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
SOUZA JÚNIOR, L. de; LOURENÇO JÚNIOR, J. de B.; SANTOS, N. de F. A. dos; ARAÚJO, C. V. de; FATURI, C.; NAHUM, B. de S. |
Afiliação: |
Lucival de Souza Júnior; José de Brito Lourenço Júnior, CPATU; Núbia de Fátima Alves dos Santos, Bolsista Capes; Cláudio Vieira de Araújo, UFRA; Cristian Faturi, UFRA; BENJAMIM DE SOUZA NAHUM, CPATU. |
Título: |
Análise da digestibilidade aparente da proteína bruta e fibra em detergente neutro do subproduto torta de coco (Cocos nucifera L.) na dieta de ovinos. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 5.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 11.; SIMPÓSIO SERGIPANO DE PRODUÇÃO ANIMAL, 1., 2008, Aracaju. Anais... Aracaju: Sociedade Nordestina de Produção Animal; Embrapa Tabuleiros Costeiros, 2008. |
Idioma: |
Português |
Thesagro: |
Dieta; Digestibilidade Aparente; Ovino; Ovinocultura; Pecuária; Ruminante; Valor Nutritivo; Zootecnia. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/102280/1/5879.pdf
|
Marc: |
LEADER 01034nam a2200253 a 4500 001 1408811 005 2022-11-23 008 2008 bl uuuu u00u1 u #d 100 1 $aSOUZA JÚNIOR, L. de 245 $aAnálise da digestibilidade aparente da proteína bruta e fibra em detergente neutro do subproduto torta de coco (Cocos nucifera L.) na dieta de ovinos.$h[electronic resource] 260 $aIn: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 5.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 11.; SIMPÓSIO SERGIPANO DE PRODUÇÃO ANIMAL, 1., 2008, Aracaju. Anais... Aracaju: Sociedade Nordestina de Produção Animal; Embrapa Tabuleiros Costeiros$c2008 650 $aDieta 650 $aDigestibilidade Aparente 650 $aOvino 650 $aOvinocultura 650 $aPecuária 650 $aRuminante 650 $aValor Nutritivo 650 $aZootecnia 700 1 $aLOURENÇO JÚNIOR, J. de B. 700 1 $aSANTOS, N. de F. A. dos 700 1 $aARAÚJO, C. V. de 700 1 $aFATURI, C. 700 1 $aNAHUM, B. de S.
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