Data/hora: 17/06/2024 10:54:58

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 15/02/2017
Data da última atualização: | 29/12/2017
Autoria: | SANTOS, M. L. T.; BORGES, A. A.; QUEIROZ NETA, L. B.; SANTOS, M. V. O.; OLIVEIRA, M. F.; SILVA, A. R.; PEREIRA, A. F.
Afiliação: | MAGDA L. T. SANTOS, UFERSA; ALANA A. BORGES, UFERSA; LUIZA B. QUEIROZ NETA, UFERSA; MARIA V. O. SANTOS, UFERSA; MOACIR F. OLIVEIRA, UFERSA; ALEXANDRE R. SILVA, UFERSA; ALEXANDRA F. PEREIRA, UFERSA.
Título: | In vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements.
Ano de publicação: | 2016
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 12, p. 1194-1202, dez. 2016.
Idioma: | Português
Conteúdo: | The maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57?0.55 vs. FBS20: 0.82?0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve the quality of growing these cells. To our knowledge, this is the first study culturing somatic cells of collared peccaries.
Palavras-Chave: | Mitotic factor; Somatic tissue.
Thesagro: | Tayassu Tajacu.
Thesaurus Nal: | Culture media; Protein sources; Somatic cells; Wild animals.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/155804/1/In-vitro-culutre-of-somatic.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (60829-1) - UPE - AP - DD -  -  -  - DIS