Data/hora: 23/06/2024 22:01:03

Biblioteca(s): | Embrapa Rondônia.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 2
Data corrente: | 22/09/2016
Data da última atualização: | 06/10/2016
Autoria: | SANTOS, M. R. A. dos; SOUZA, C. A. de.
Afiliação: | MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia; Carolina Augusto de Souza.
Título: | Dedifferentiation of Leaf Cells and Growth Pattern of Calluses of Capsicum annuumcv. Etna.
Ano de publicação: | 2016
Fonte/Imprenta: | Australian Journal of Basic and Applied Sciences, v. 10, n. 12, p. 362-368, 2016.
Idioma: | Inglês
Conteúdo: | Background:In vitrocell suspension cultivation systems have been largely reported assafe and standardized methods for production of secondary metabolites with medicinaland agricultural interest.Capsicum annuumis one of the most widely grown vegetablein the world and its biological activities have been demonstrated against insects, fungi,bacteria and other groups of organisms. The determination of procedures for thededifferentiation of cells into callus cells and the subsequent study of the callus growthpattern are necessary for the establishment of cellsuspensions and also to subsidizestudies regarding the bioactivity of its secondarymetabolites. To date, no study hasdescribed the development of protocols for callus induction inC. annuumL. cv. Etna. Objective:The objective of this study was to establish a protocol for dedifferentiationof leaf cells of the cultivarC. annuumcv. Etna and to determine the growth pattern ofthe calluses with a focus on the deceleration phase, when the callus cells must besubcultured into a liquid medium in order to establish cell suspension cultivationsaiming at the production of secondary metabolites.Results:The treatment that resultedin the highest %CI, ACCC and callus weight was thecombination of 4.52 &#956; M 2,4-D +0.44 &#956; M BA. The calluses produced were friable andwhitish and their growth patternfollowed a sigmoid shape. The deceleration phase started on the 23rdday of cultivation.Conclusion:Callus induction in leaf explants ofC. annuumcv. Etnacan be achieved inMS medium supplemented with 4.52 &#956; M 2,4-D + 0.44 &#956; MBA, which results in highcellular proliferation; in order to start a cell suspension culture, callus cells on the 23rdday of culture should be used.
Palavras-Chave: | Callogenesis; Calogêneses; Growth curve; Metabolismo secundário.
Thesagro: | Curva de Crescimento; Pimenta.
Thesaurus Nal: | secondary metabolites.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/147697/1/Differentiation-SANTOS-2016.pdf
Registro original: | Embrapa Rondônia (CPAF-RO)
Exemplares: | CPAF-RO - (17714-1) - UPC - AP - DD -  -  -  - DIS