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| Registros recuperados : 31 | |
| 21. |  | PORTO, B. N.; ALVES, J. D.; MAGALHAES, P. C.; CASTRO, E. M.; CAMPOS, N. A.; SOUZA, K. R. D.; MAGALHAES, M. M.; ANDRADE, C. A.; SANTOS, M. O. Calcium-dependent tolerant responses of cell wall in maize mesocotyl flooding stress. Journal of Agronomy and Crop Science, v. 199, p. 134-143, 2013.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 22. | | PANDELO, D.; MELO, T. D.; SINGULANI, J. L.; GUEDES, F. A. F.; MACHADO, M. A.; COELHO, C. M.; VICCINI, L. F.; SANTOS, M. O. Oil production at different stages of leaf development in Lippia alba. Revista Brasileira de Farmacognosia, v. 22, n. 3, p. 497-501, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 23. | | MOREIRA, J. S.; ALMEIDA, R. G.; SANTOS, M. O.; VASCONCELOS, I. M.; OLIVEIRA, J. T. A.; RIBEIRO, S. da G.; MARCELLINO, L. H.; DIAS, S. C.; FRANCO, O. L. Molecular characterization of two antifungal proteins from flowers of Rosemay pepper (Lippia Sidoides) with activity against botrytis cinerea. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 90.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 24. | | NUNES, P. H.; PIERANGELI, E. V.; SANTOS, M. O.; SILVEIRA, H. R. O.; MATOS, C. S. M. de; PEREIRA, A. B.; ALVES, H. M. R.; VOLPATO, M. M. L.; SILVA, V. A.; FERREIRA, D. D. Predicting coffee water potential from spectral reflectance indices with neural networks. Smart Agricultural Technology, v. 4, 100213, 2023. 6 p.| Biblioteca(s): Embrapa Café. |
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| 25. | | SANTOS, M. O.; SASSAKI, R. P.; FERREIRA, T. H. S.; RESENDE, R. M. S.; CHIARI, L.; KARIA, C. T.; FALEIRO, F. G.; JUNGMANN, L.; ZUCCHI, M. I.; SOUZA, A. P. Polymorphic microsatellite loci for Stylosanthes macrocephala Ferr. et Costa, a tropical forage legume Conservation Genetics Resources, v. 1, p. 481-485, 2009.| Biblioteca(s): Embrapa Cerrados. |
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| 26. | | SANTOS, M. O.; SASSAKI, R. P.; FERREIRA, T. H. S.; RESENDE, R. M. S.; CHIARI, L.; KARIA, C. T.; FALEIRO, F. G.; JUNGMANN, L.; ZUCCHI, M. I.; SOUZA, A. P. Polymorphic microsatellite loci for Stylosanthes macrocephala Ferr. et Costa, a tropical forage legume Conservation Genetics Resources, v.1, p. 481-485, 2009.| Biblioteca(s): Embrapa Gado de Corte. |
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| 27. | | AMARAL, D. L.; ZANETTE, R. S.; ALMEIDA, C. G.; ALMEIDA, L. B.; OLIVEIRA, L. F.; MARCOMINI, R. F.; NOGUEIRA, B. V.; SANTOS, M. O.; BRANDAO, H. de M.; MARANDUBA, C. M. C.; MUNK, M. In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation. Biomedical Materials, v. 14, article 035011, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| 28. | | MACIEL, D. A.; SILVA, V. A.; ALVES, H. M. R.; VOLPATO, M. M. L.; BARBOSA, J. P. R. A. de; SOUZA, V. C. O.; SANTOS, M. O.; SILVEIRA, H. R. DE O.; DANTAS, M. F.; FREITAS, A. F. de; SANTOS, J. O. DOS. Leaf water potential of coffee estimated by landsat-8 images. Plos One, v. 15, n. 3, e031019, Mar. 2020.| Biblioteca(s): Embrapa Café. |
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| 29. | | LIMA, A. R. de S.; SOUZA, V. L. N. de; PEREIRA, M. C. N.; BRITO, M. A. A.; CARNEIRO, E. de F.; RIBEIRO, A. B. de S.; OLIVEIRA, G. M. T. de; PIEROZZI JUNIOR, I.; TUPINAMBÁ, M. J. F.; BERNI, R. F.; ROCHA, A. S. N. da C.; SANTOS, M. O. dos. Gestão integrada em negócios e comunicação na Embrapa Amazônia Ocidental. In: REUNIÃO TÉCNICA DE PESQUISA E DESENVOLVIMENTO DA EMBRAPA AMAZÔNIA OCIDENTAL, 4., 2009, Manaus. Anais... Manaus: Embrapa Amazônia Ocidental, 2009. p. 172-174. (Embrapa Amazônia Ocidental. Documentos, 73).| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 30. | | LIMA, A. R. de S.; SOUZA, V. L. N. de; PIEROZZI JUNIOR, I.; LOPES, N. C.; CARVALHO, E. de F. C.; BRITO, M. A. A.; PEREIRA, M. C. N.; OLIVEIRA, G. M. T. de; RIBEIRO, A. B. de S.; TUPINAMBÁ, M. J. F.; BERNI, R. F.; ROCHA, A. S. N. da C.; SANTOS, M. O. dos. Gestão integrada em negócios e comunicação - Ginc. In: REUNIÃO TÉCNICA DE PESQUISA E DESENVOLVIMENTO DA EMBRAPA AMAZÔNIA OCIDENTAL, 5., 2011, Manaus. Anais... Manaus: Embrapa Amazônia Ocidental, 2011. p. 18-19. (Embrapa Amazônia Ocidental. Documentos, 87).| Biblioteca(s): Embrapa Agricultura Digital. |
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| 31. | | LIMA, A. R. de S.; SOUZA, V. L. N. de; PIEROZZI JUNIOR, I.; LOPES, N. C.; CARVALHO, E. de F. C.; SOUSA, M. A. A. B. de; PEREIRA, M. C. N.; OLIVEIRA, G. M. T. de; RIBEIRO, A. B. de S.; TUPINAMBA, M. J. F.; BERNI, R. F.; ROCHA, A. S. N. da C.; SANTOS, M. O. dos. Gestão integrada em negócios e comunicação - Ginc. In: REUNIÃO TÉCNICA DE PESQUISA E DESENVOLVIMENTO DA EMBRAPA AMAZÔNIA OCIDENTAL, 5., 2011, Manaus. Anais... Manaus: Embrapa Amazônia Ocidental, 2011. p. 18-19. (Embrapa Amazônia Ocidental. Documentos, 87).| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| Registros recuperados : 31 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
13/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
AMARAL, D. L.; ZANETTE, R. S.; ALMEIDA, C. G.; ALMEIDA, L. B.; OLIVEIRA, L. F.; MARCOMINI, R. F.; NOGUEIRA, B. V.; SANTOS, M. O.; BRANDAO, H. de M.; MARANDUBA, C. M. C.; MUNK, M. |
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Afiliação: |
HUMBERTO DE MELLO BRANDAO, CNPGL. |
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Título: |
In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Biomedical Materials, v. 14, article 035011, 2019. |
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DOI: |
https://doi.org/10.1088/1748-605x/ab0a52 |
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Idioma: |
Inglês |
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Conteúdo: |
Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering. In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation. In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC). Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays. Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity. The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation. Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold. Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements. These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation. MenosNanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering. In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation. In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC). Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays. Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity. The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation. Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold. Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements. These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic... Mostrar Tudo |
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Palavras-Chave: |
3D scaffolds; Human stem cells; Nanomaterial; Nanotopography; Osteoinduction. |
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Categoria do assunto: |
-- |
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Marc: |
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100 1 $aAMARAL, D. L.
245 $aIn vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation.$h[electronic resource]
260 $c2019
520 $aNanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering. In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation. In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC). Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays. Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity. The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation. Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold. Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements. These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation.
653 $a3D scaffolds
653 $aHuman stem cells
653 $aNanomaterial
653 $aNanotopography
653 $aOsteoinduction
700 1 $aZANETTE, R. S.
700 1 $aALMEIDA, C. G.
700 1 $aALMEIDA, L. B.
700 1 $aOLIVEIRA, L. F.
700 1 $aMARCOMINI, R. F.
700 1 $aNOGUEIRA, B. V.
700 1 $aSANTOS, M. O.
700 1 $aBRANDAO, H. de M.
700 1 $aMARANDUBA, C. M. C.
700 1 $aMUNK, M.
773 $tBiomedical Materials$gv. 14, article 035011, 2019.
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