Data/hora: 19/06/2024 04:04:24

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 02/06/2022
Data da última atualização: | 02/06/2022
Autoria: | SANTOS, E. M. dos; TAVARES, L. S.; FAYER, L.; BRANDAO, H. de M.; MUNK, M.; SANTOS, M. de O.
Afiliação: | ELISANDRA M. DOS SANTOS, Universidade Federal de Juiz de Fora; LETÍCIA S. TAVARES, Universidade Federal de Juiz de Fora; LEONARA FAYER, Universidade Federal de Juiz de Fora; HUMBERTO DE MELLO BRANDAO, CNPGL; MICHELE MUNK, Universidade Federal de Juiz de Fora; MARCELO DE O. SANTOS, Universidade Federal de Juiz de Fora.
Título: | Nanoencapsulated Lippia rotundifolia antimicrobial peptide: synthesis, characterization, antimicrobial activity, and cytotoxicity evaluations.
Ano de publicação: | 2022
Fonte/Imprenta: | Archives of Microbiology, v. 204, n. 3, 184, 2022.
DOI: | https://doi.org/10.1007/s00203-022-02787-z
Idioma: | Inglês
Conteúdo: | Antimicrobial peptides (AMP) are promising novel antibiotics but exhibit low stability and can be toxic. The AMP encapsulation can be used to protect the drug and control its release rates. The Lr-AMP1f encapsulated into chitosan nanoparticle (NP) by ionic gelation method reached 90% efficiency. The results indicated that the hydrodynamic particle size of NPs increased from 196.1 ± 3.14 nm (free NP) to 228.1 ± 12.22 nm (nanoencapsulated Lr-AMP1f), while the atomic force microscope showed the spherical shape. The Zeta potential of the nanoencapsulated Lr-AMP1f was high (+ 35 mV). These AMP-loaded NPs exhibited stability for up to 21 days of storage. The minimum inhibitory concentration (MIC) of free Lr-AMP1f was 8 µg/mL for E. coli and S. epidermidis. However, the nanoencapsulated Lr-AMP1f produced a bacteriostatic effect against both bacteria at 8 µg/mL. The MIC of nanoencapsulated Lr-AMP1f was 16 µg/mL for E. coli and 32 for S. epidermidis. Nanoencapsulated Lr-AMP1f was nontoxic to HEK293 cells. Promisingly, chitosan NP can be used as a vehicle for the antibacterial application of new AMP (Lr-AMP1f).
Palavras-Chave: | Atividade antimicrobiana; Citotoxicidade; Nanocapsulado; Nanotecnologia; Peptídeo antimicrobiano.
Thesagro: | Antibiótico; Peptídeo.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (25668-1) - UPC - AP - DD -  -  -  - DIS