Data/hora: 16/06/2024 22:47:40

Biblioteca(s): 	Embrapa Caprinos e Ovinos; Embrapa Gado de Leite.	
Data corrente: 	12/01/2021	
Data da última atualização: 	15/09/2021	
Autoria: 	BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; ALFRADIQUE, V. A. P.; ARASHIRO, E. K. N.; PINTO, P. H. N.; SANTOS, J. D. R.; CAMARGO, L. S. de A.; MENCHACA, A.; FONSECA, J. F. da; BRANDÃO, F. Z.	
Afiliação: 	Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Fundacion IRAUy, Cno. Cruz del Sur, Montevideo, Uruguay; JEFERSON FERREIRA DA FONSECA, CNPC; Universidade Federal Fluminense.	
Título: 	Exogenous progestogens differentially alter gene expression of immature cumulus-oocyte complexes in sheep.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Domestic Animal Endocrinology, v. 74, e106518, 2021.	
DOI: 	https://doi.org/10.1016/j.domaniend.2020.106518	
Idioma: 	Inglês	
Conteúdo: 	Abstracts: This study evaluated the role of progesterone (P4) and medroxyprogesterone acetate (MAP) on the molecular status of immature cumulus-oocyte complexes (COCs) and the implications for oocyte quality in sheep. The number of viable COCs per ewe and the rate of COCs screened for developmental competence by brilliant cresyl blue positive (BCBþ) were similar (P > 0.05), respectively, across treatments (P4: 7.7 ± 0.7 and 4.7 ± 1.2; MAP: 5.7 ± 1.0 and 3.5 ± 2.3; and control: 5.7 ± 1.1 and 3.6 ± 2.4). The COCs' gene expression was altered by exogenous progestogens compared with the control group: markers of steroidogenic pathway (FSH receptor [FSHr], LH receptor [LHr], and estradiol receptor a) and of quality (zygote arrest 1, growth differentiation factor 9, and B-cell lymphoma 2) were in abundance in P4 (P < 0.05). In addition, reelin protein (RELN) was downregulated, and Bcl-2 was upregulated in MAP (P < 0.05). In the P4 vs MAP comparison, FSHr, LHr, and RELN genes were upregulated (P < 0.05) in the P4 group. In conclusion, P4 and MAP promoted dissimilar effects on transcriptome profiling of immature BCB-selected COCs, possibly due to the differences in the chemical structure of progestogens and concentrations of serum P4. Exogenous P4 impacted positively on the profile of genes related to oocyte quality.	
Palavras-Chave: 	Oocyte competence; Progestins.	
Thesagro: 	Ovelha; Ovino; Reprodução Animal.	
Thesaurus Nal: 	Ewes; Gene expression; Medroxyprogesterone; Oocytes; Progesterone; Small ruminants.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPC - (39743-1) - UPC - AP - DD -  -  -  - DIS
CNPGL - (25149-1) - UPC - AP - DD -  -  -  - DIS