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21. | | TOSCANO, J. H. B.; OKINO, C. H.; SANTOS, I. B. dos; GIRALDELO, L. A.; HAEHLING, M. B. von; ESTEVES, S. N.; BASSETTO, C. C.; AMARANTE, A. F. T. do; CHAGAS, A. C. de S. Local and systemic immune mediators of Morada Nova lambs with divergent Haemonchus contortus resistance phenotypes. Parasite Immunology, v. 42, e12790, 2020. 10 p. Biblioteca(s): Embrapa Pecuária Sudeste. |
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22. | | OKINO, C. H.; NICIURA, S. C. M.; GIARETTA, P. R.; MELITO, G. R.; KAPRITCHKOFF, R. T. I.; SANTOS, I. B. DOS; RECH, R. R.; MINHO, A. P.; ESTEVES, S. N.; CHAGAS, A. C. de S. Enhanced mucosal response in sheep harbouring BetaA beta-globin against haemonchosis. Parasite Immunology, v. 45, n. 9, e13003, sep. 2023. 12 p. Biblioteca(s): Embrapa Pecuária Sudeste. |
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23. | | MATTOS, M. L. T.; MARTINS, J. F. da S.; MAGALHÃES JÚNIOR, A.; PETRINI, J. A.; SILVA, J. J. C. da; SCIVITTARO, W. B.; NUNES, C. D. M.; CUNHA, N. G. da; SANTOS, I. B. dos. Monitoramento físico-químico da qualidade da água em áreas piloto da produção integrada de arroz irrigado na planície costeira externa do Rio Grande do Sul. Pelotas: Embrapa Clima Temperado, 2008. 5 p. (Embrapa Clima Temperado. Comunicado técnico, 202). Biblioteca(s): Embrapa Unidades Centrais. |
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24. | | MATTOS, M. L. T.; MARTINS, J. F. de S.; MAGALHÃES JÚNIOR, A.; PETRINI, J. A.; SILVA, J. J. C. da; SCIVITTARO, W. B.; NUNES, C. D. M.; CUNHA, N. G. da; SANTOS, I. B. dos. Monitoramento físico-químico da qualidade da água em áreas piloto da produção integrada de arroz irrigado na planície costeria externa do Rio Grande do Sul. Pelotas: Embrapa Clima Temperado, 2008. 5 p. (Embrapa Clima Temperado. Comunicado técnico, 202). Biblioteca(s): Embrapa Clima Temperado. |
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26. | | OKINO, C. H.; CAMINOTTO, L. C. F.; KAPRITCHKOFF, R. T. I.; NICIURA, S. C. M.; ESTEVES, S. N.; SANTOS, I. B. DOS; TOSCANO, J. H. B.; OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S. Comparison of ovine B-globin haplotype sequences and a new multiplex PCR for identification. Veterinary Parasitology, v.300, 109592, dec. 2021. 5 p. Biblioteca(s): Embrapa Pecuária Sudeste. |
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27. | | CAMINOTTO, L. C. F.; KAPRITCHKOFF, R. T. I.; NICIURA, S. C. M.; ESTEVES, S. N.; SANTOS, I. B. DOS; OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S.; OKINO, C. H. Desenvolvimento de PCR para identificação de haplótipos da B-globina ovina e determinação de frequência alélica na raça Morada Nova . In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 13., 2021, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2021. p.21. (Embrapa Pecuária Sudeste. Documentos, 140). Biblioteca(s): Embrapa Pecuária Sudeste. |
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28. | | OKINO, C. H.; TOSCANO, J. H. B; GIRALDELO, L. A.; VON HAEHLING, M. B.; SANTOS, I. B. dos; ESTEVES, S. N.; FERRAZ JUNIOR, R. S.; CHAGAS, A. C. de S. Differential local immune responses against Haemonchus contortus in a resistant Brazilian sheep breed (Morada Nova). In: INTERNATIONAL VETERINARY IMMUNOLOGY SYMPOSIUM, 12., 2019, Seattle. proceedings... Seattle: AAVI, 2019. 114. Biblioteca(s): Embrapa Pecuária Sudeste. |
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29. | | HAEHLING, M. B. V.; CRUVINEL, G. G.; TOSCANO, J. H. B.; GIRALDELO, L. A.; SANTOS, I. B. dos; ESTEVES, S. N.; BENAVIDES, M. V.; BARIONI JUNIOR, W.; NICIURA, S. C. M.; CHAGAS, A. C. de S. Four single nucleotide polymorphisms (SNPs) are associated with resistance and resilience to Haemonchus contortus in Brazilian Morada Nova sheep. Veterinary Parasitology, v. 279, 2020, 109053. 11 p. Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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30. | | OKINO, C. H.; NICIURA, S. C. M.; TOSCANO, J. H. B.; ESTEVES, S. N.; SANTOS, I. B. DOS; HAEHLING, M. B. VON; FIGUEIREDO, A.; OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S. Ovine B-globin gene: a new qPCR for rapid haplotype identification and association with susceptibility to Haemonchus contortus infection. Veterinary Parasitology, v.294, june, 109434, 2021. 6 p. Biblioteca(s): Embrapa Pecuária Sudeste. |
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31. | | TOSCANO, J. H. B.; OKINO, C. H.; VON HAEHLING; SANTOS, I. B. dos; GIRALDELO, L. A.; FIGUEIREDO, A.; ESTEVES, S. N.; FERRAZ JUNIOR, R. S.; BASSETO, C. C.; AMARANTE, F. T. do; CHAGAS, A. C. de S. Systemic immune responses associated to Haemonchus contortus resistance in a resistant Brazilian sheep breed (Morada Nova). In: INTERNATIONAL VETERINARY IMMUNOLOGY SYMPOSIUM, 12., 2019, Seattle. Proceedings... Seattle: AAVI, 2019. p.119. Biblioteca(s): Embrapa Pecuária Sudeste. |
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32. | | CHAGAS, A. C. de S.; SANTOS, I. B. DOS; ALEMAN GAINZA, Y.; SOUZA, G. A. DE; ANHOLETO, L. A.; BARIONI JUNIOR, W.; ESTEVES, S. N.; NICIURA, S. C. M. Tratamento anti-helmíntico seletivo de ovinos pelo peso (TST) visando assegurar a eficácia dos fármacos desparasitantes e a segurança dos produtos. In: CONGRESSO NACIONAL DA CAPRINICULTURA, 1., 2021. Evento online: APEZ, 2021. p. 128-149. Biblioteca(s): Embrapa Pecuária Sudeste. |
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33. | | SANTOS, I. B. dos; MACIEL, W. G.; FELIPPELLI, G.; TOSCANO, J. H. B.; CRUZ, B. C.; CHAGAS, A. C. de S.; SOARES, V. E.; LOPES. W. D. Z.; COSTA, A. J. da; OLIVEIRA, G. P. de. Viability of Haemonchus placei parasitism in experimentally infected young goats. Veterinary Parasitology, v. 271, p. 64-67, 2019. Biblioteca(s): Embrapa Pecuária Sudeste. |
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34. | | SANTOS, I. B. DOS; ANHOLETO, L. A.; SOUSA, G. A. DE; NUCCI, A. DA S.; ALEMÁN GAINZA, Y.; FIGUEIREDO A.; SANTOS, L. A. L. DOS; MINHO, A. P.; BARIONI JUNIOR, W.; ESTEVES, S. N.; NICIURA, S. C. M.; CHAGAS, A. C. de S. Investigating the benefits of targeted selective treatment according to average daily weight gain against gastrointestinal nematodes in Morada Nova lambs. Parasitology Research, 2022. 12 p. Onlie First.
https://rdcu.be/cOwc0 Biblioteca(s): Embrapa Pecuária Sudeste. |
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Registros recuperados : 34 | |
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Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
03/05/2021 |
Data da última atualização: |
10/05/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
OKINO, C. H.; NICIURA, S. C. M.; TOSCANO, J. H. B.; ESTEVES, S. N.; SANTOS, I. B. DOS; HAEHLING, M. B. VON; FIGUEIREDO, A.; OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S. |
Afiliação: |
CINTIA HIROMI OKINO, CPPSE; SIMONE CRISTINA MEO NICIURA, CPPSE; JOÃO HENRIQUE BARBOSA TOSCANO, UNESP; SERGIO NOVITA ESTEVES, CPPSE; ISABELLA BARBOSA DOS SANTOS, UNESP; MAREI BORSCH VON HAEHLING, UNESP; AMANDA FIGUEIREDO, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
Título: |
Ovine B-globin gene: a new qPCR for rapid haplotype identification and association with susceptibility to Haemonchus contortus infection. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Veterinary Parasitology, v.294, june, 109434, 2021. |
Páginas: |
6 p. |
DOI: |
https://doi.org/10.1016/j.vetpar.2021.109434 |
Idioma: |
Inglês |
Conteúdo: |
Two B-globin allelic haplotypes (A and B) were identified in domestic sheep, wherein animals which are homozygous for B B allele (BB haplotype) have a deletion of pre-adult B C-globin and consequently are less tolerant to anemia and hypoxia. Since Haemonchus contortus infection, is associated with severe anemia, studies performed from 1960s to 1990s investigated the association between B-globin haplotype and resistance against this parasite. However, the findings were controversial, pointing out from increased resistance in animals harboring the B A allele to inexistence of association. Thus, our study aimed to develop a qPCR for B-globin haplotype identification, and to evaluate the association between B-globin haplotype and resistance against H. contortus in a group of sheep submitted to artificial infection with this parasite. A total of 286 lambs of Morada Nova breed were experimentally challenged with 4000 H. contortus L3 and monitored for 112 days from weaning. Significantly improved (p < 0.05) phenotypic profiles (lower fecal egg counts, higher packed cell volume and birthweight) were observed for AA haplotype animals, especially when compared to BB animals, while AB animals were similar to BB. This is the first report of a qPCR assay for ovine B-globin haplotype identification. In view of significant differences of phenotypic profiles between haplotype groups, the developed qPCR may constitute an important tool for sheep producers to improve genetic selection of parasite resistant animals. MenosTwo B-globin allelic haplotypes (A and B) were identified in domestic sheep, wherein animals which are homozygous for B B allele (BB haplotype) have a deletion of pre-adult B C-globin and consequently are less tolerant to anemia and hypoxia. Since Haemonchus contortus infection, is associated with severe anemia, studies performed from 1960s to 1990s investigated the association between B-globin haplotype and resistance against this parasite. However, the findings were controversial, pointing out from increased resistance in animals harboring the B A allele to inexistence of association. Thus, our study aimed to develop a qPCR for B-globin haplotype identification, and to evaluate the association between B-globin haplotype and resistance against H. contortus in a group of sheep submitted to artificial infection with this parasite. A total of 286 lambs of Morada Nova breed were experimentally challenged with 4000 H. contortus L3 and monitored for 112 days from weaning. Significantly improved (p < 0.05) phenotypic profiles (lower fecal egg counts, higher packed cell volume and birthweight) were observed for AA haplotype animals, especially when compared to BB animals, while AB animals were similar to BB. This is the first report of a qPCR assay for ovine B-globin haplotype identification. In view of significant differences of phenotypic profiles between haplotype groups, the developed qPCR may constitute an important tool for sheep producers to improve genetic selection of para... Mostrar Tudo |
Palavras-Chave: |
Allele; B haplotype; Beta globin C; Morada Nova. |
Thesaurus NAL: |
Sheep. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02450naa a2200301 a 4500 001 2131645 005 2021-05-10 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.vetpar.2021.109434$2DOI 100 1 $aOKINO, C. H. 245 $aOvine B-globin gene$ba new qPCR for rapid haplotype identification and association with susceptibility to Haemonchus contortus infection.$h[electronic resource] 260 $c2021 300 $a6 p. 520 $aTwo B-globin allelic haplotypes (A and B) were identified in domestic sheep, wherein animals which are homozygous for B B allele (BB haplotype) have a deletion of pre-adult B C-globin and consequently are less tolerant to anemia and hypoxia. Since Haemonchus contortus infection, is associated with severe anemia, studies performed from 1960s to 1990s investigated the association between B-globin haplotype and resistance against this parasite. However, the findings were controversial, pointing out from increased resistance in animals harboring the B A allele to inexistence of association. Thus, our study aimed to develop a qPCR for B-globin haplotype identification, and to evaluate the association between B-globin haplotype and resistance against H. contortus in a group of sheep submitted to artificial infection with this parasite. A total of 286 lambs of Morada Nova breed were experimentally challenged with 4000 H. contortus L3 and monitored for 112 days from weaning. Significantly improved (p < 0.05) phenotypic profiles (lower fecal egg counts, higher packed cell volume and birthweight) were observed for AA haplotype animals, especially when compared to BB animals, while AB animals were similar to BB. This is the first report of a qPCR assay for ovine B-globin haplotype identification. In view of significant differences of phenotypic profiles between haplotype groups, the developed qPCR may constitute an important tool for sheep producers to improve genetic selection of parasite resistant animals. 650 $aSheep 653 $aAllele 653 $aB haplotype 653 $aBeta globin C 653 $aMorada Nova 700 1 $aNICIURA, S. C. M. 700 1 $aTOSCANO, J. H. B. 700 1 $aESTEVES, S. N. 700 1 $aSANTOS, I. B. DOS 700 1 $aHAEHLING, M. B. VON 700 1 $aFIGUEIREDO, A. 700 1 $aOLIVEIRA, M. C. de S. 700 1 $aCHAGAS, A. C. de S. 773 $tVeterinary Parasitology$gv.294, june, 109434, 2021.
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