| |
|
|
| Registros recuperados : 556 | |
| Registros recuperados : 556 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
28/07/2010 |
|
Data da última atualização: |
18/08/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
LOPES, M. A.; HORA JUNIOR, B. T.; DIAS, C. V.; SANTOS, G. C.; GRAMACHO, K. P.; CASCARDO, J. C. M.; GESTEIRA, A. da S.; MICHELI, F. |
|
Afiliação: |
Karina Peres Gramacho, CEPLAC; Júlio Cézar Mattos Cascardo, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; Fabienne Micheli, UESC. |
|
Título: |
Expression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae). |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
Genetics and Molecualr Reseach, Ribeirão Preto, v. 9, n. 3, p. 1279-1297, 2010. |
|
ISSN: |
1676-5680 |
|
DOI: |
10.4238/vol9-3gmr825 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Cacao (Theobroma cacao) is one of the most important tropical crops; however, production is threatened by numerous pathogens, including the hemibiotrophic fungus Moniliophthora perniciosa, which causes witches? broom disease. To understand the mechanisms that lead to the development of this disease in cacao, we focused our attention on cacao transcription factors (TFs), which act as master regulators of cellular processes and are important for the fine-tuning of plant defense responses. We developed a macroarray with 88 TF cDNA from previously obtained cacao-M. perniciosa interaction libraries. Seventy-two TFs were found differentially expressed between the susceptible (Catongo) and resistant (TSH1188) genotypes and/or during the disease time course - from 24 h to 30 days after infection. Most of the differentially expressed TFs belonged to the bZIP, MYB and WRKY families and presented opposite expression patterns in susceptible and resistant cacao-M. perniciosa interactions (i.e., up-regulated in Catongo and down-regulated in TSH1188). The results of the macroarray were confirmed for bZIP and WRKY TFs by real-time PCR. These differentially expressed TFs are good candidates for subsequent functional analysis as well as for plant engineering. Some of these TFs could also be localized on the cacao reference map related to witches? broom resistance, facilitating the breeding and selection of resistant cacao trees. |
|
Palavras-Chave: |
Gene regulation; Macroarrays; RT-qPCR; Theobrama cacao; Witches broom disease. |
|
Thesagro: |
Cacau; Doença de Planta; Fungo; Genótipo; Hibridação; Vassoura de Bruxa. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/858659/1/gmr825.pdf
|
|
Marc: |
LEADER 02501naa a2200361 a 4500
001 1858659
005 2022-08-18
008 2010 bl uuuu u00u1 u #d
022 $a1676-5680
024 7 $a10.4238/vol9-3gmr825$2DOI
100 1 $aLOPES, M. A.
245 $aExpression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae).$h[electronic resource]
260 $c2010
520 $aCacao (Theobroma cacao) is one of the most important tropical crops; however, production is threatened by numerous pathogens, including the hemibiotrophic fungus Moniliophthora perniciosa, which causes witches? broom disease. To understand the mechanisms that lead to the development of this disease in cacao, we focused our attention on cacao transcription factors (TFs), which act as master regulators of cellular processes and are important for the fine-tuning of plant defense responses. We developed a macroarray with 88 TF cDNA from previously obtained cacao-M. perniciosa interaction libraries. Seventy-two TFs were found differentially expressed between the susceptible (Catongo) and resistant (TSH1188) genotypes and/or during the disease time course - from 24 h to 30 days after infection. Most of the differentially expressed TFs belonged to the bZIP, MYB and WRKY families and presented opposite expression patterns in susceptible and resistant cacao-M. perniciosa interactions (i.e., up-regulated in Catongo and down-regulated in TSH1188). The results of the macroarray were confirmed for bZIP and WRKY TFs by real-time PCR. These differentially expressed TFs are good candidates for subsequent functional analysis as well as for plant engineering. Some of these TFs could also be localized on the cacao reference map related to witches? broom resistance, facilitating the breeding and selection of resistant cacao trees.
650 $aCacau
650 $aDoença de Planta
650 $aFungo
650 $aGenótipo
650 $aHibridação
650 $aVassoura de Bruxa
653 $aGene regulation
653 $aMacroarrays
653 $aRT-qPCR
653 $aTheobrama cacao
653 $aWitches broom disease
700 1 $aHORA JUNIOR, B. T.
700 1 $aDIAS, C. V.
700 1 $aSANTOS, G. C.
700 1 $aGRAMACHO, K. P.
700 1 $aCASCARDO, J. C. M.
700 1 $aGESTEIRA, A. da S.
700 1 $aMICHELI, F.
773 $tGenetics and Molecualr Reseach, Ribeirão Preto$gv. 9, n. 3, p. 1279-1297, 2010.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
