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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
11/06/2010 |
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Data da última atualização: |
04/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VALICENTE, F. H.; PICOLI, E. A. de T.; VASCONCELOS, M. J. V. de; CARNEIRO, N. P.; CARNEIRO, A. A.; GUIMARAES, C. T.; LANA, U. G. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS; EDGAR AUGUSTO DE TOLEDO PICOLI, CNPMS; MARIA JOSE VILACA DE VASCONCELOS, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS; ANDREA ALMEIDA CARNEIRO, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS. |
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Título: |
Molecular characterization and distribution of Bacillus thuringiensis cry1 genes from Brazilian strains effective against the fall armyworm, Spodoptera frugiperda. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Biological Control, San Diego, v. 53, p. 360-366, 2010. |
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DOI: |
10.1016/j.biocontrol.2010.02.003 |
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Idioma: |
Inglês |
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Conteúdo: |
A total of 165 Bacillus thuringiensis (Bt) strains that showed larval mortality above 75% were selected from 4459 isolates previously evaluated with respect to Spodoptera frugiperda activity. Molecular characterization was performed based on PCR using specific cry1 primers where 20% did not amplify the expected fragments; 62.4% amplified fragments corresponding to the presence of only one gene, and 15.2%, 1.8% and 0.6% presented a profile of two, three and four different cry1 genes, respectively. SDS?PAGE protein analyses were in accordance with the presence of cry1 genes. The most frequent gene (57.5%) was cry1D, whereas the least frequent genes were cry1Aa/cry1Ad and cry1C (1.2%). However, more than 60% of the evaluated strains had cry1B or cry1E genes. Analyzing cry1 genes and the larval mortality together, the range of activity from the most to least lethal to S. frugiperda was Bt strains with cry1C, cry1B, cry1E, cry1F, cry1A, cry1G and cry1D genes, respectively. The distribution of the strains and cry1 genes varied according to the origin of the sample and the source. DNA sequencing of the PCR products of the cry1C and cry1F genes confirmed their identities. |
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Palavras-Chave: |
Cry1 genes; Crystal protein. |
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Thesagro: |
Bacillus Thuringiensis. |
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Thesaurus Nal: |
Spodoptera. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02031naa a2200253 a 4500
001 1854885
005 2018-06-04
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1016/j.biocontrol.2010.02.003$2DOI
100 1 $aVALICENTE, F. H.
245 $aMolecular characterization and distribution of Bacillus thuringiensis cry1 genes from Brazilian strains effective against the fall armyworm, Spodoptera frugiperda.$h[electronic resource]
260 $c2010
520 $aA total of 165 Bacillus thuringiensis (Bt) strains that showed larval mortality above 75% were selected from 4459 isolates previously evaluated with respect to Spodoptera frugiperda activity. Molecular characterization was performed based on PCR using specific cry1 primers where 20% did not amplify the expected fragments; 62.4% amplified fragments corresponding to the presence of only one gene, and 15.2%, 1.8% and 0.6% presented a profile of two, three and four different cry1 genes, respectively. SDS?PAGE protein analyses were in accordance with the presence of cry1 genes. The most frequent gene (57.5%) was cry1D, whereas the least frequent genes were cry1Aa/cry1Ad and cry1C (1.2%). However, more than 60% of the evaluated strains had cry1B or cry1E genes. Analyzing cry1 genes and the larval mortality together, the range of activity from the most to least lethal to S. frugiperda was Bt strains with cry1C, cry1B, cry1E, cry1F, cry1A, cry1G and cry1D genes, respectively. The distribution of the strains and cry1 genes varied according to the origin of the sample and the source. DNA sequencing of the PCR products of the cry1C and cry1F genes confirmed their identities.
650 $aSpodoptera
650 $aBacillus Thuringiensis
653 $aCry1 genes
653 $aCrystal protein
700 1 $aPICOLI, E. A. de T.
700 1 $aVASCONCELOS, M. J. V. de
700 1 $aCARNEIRO, N. P.
700 1 $aCARNEIRO, A. A.
700 1 $aGUIMARAES, C. T.
700 1 $aLANA, U. G.
773 $tBiological Control, San Diego$gv. 53, p. 360-366, 2010.
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Embrapa Milho e Sorgo (CNPMS) |
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| 1. |  | SOUSA, E. M. de; SANTOS, E. V. dos; SILVA, E. R. O. da; SILVA, M. O. dos S.; GONÇALVES, D. de A.; PROTÁSIO, T. de P. Produção de massa seca de madeira da espécie Tachigali vulgaris L.G.Silva & H.C.Lima (tachi branco) em razão do pH do solo e espaçamento de plantio. In: CONGRESSO FLORESTAL BRASILEIRO, 9., 2022, Brasília, DF. Inovações, desafios e oportunidades para o uso sustentável, a conservação das florestas brasileiras e o desenvolvimento social: anais. Brasília, DF: SBEF, 2022. p. 90.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 2. | | MARQUES, J. D.; SANTOS, E. V. dos; LIMA, M. D. R.; PATRÍCIO, E. P. S.; GONÇALVES, D. de A.; PROTÁSIO, T. de P. Produtividade da espécie amazônica Tachigali vulgaris cultivada em plantios homogêneos com diferentes espaçamentos e idade. Série Técnica IPEF, v. 26, n. 48, p. 219-223, maio 2023. Anais do Congresso de Plantações Florestais, 2023, Piracicaba.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 3. | | SANTOS, E. V. dos; LIMA, M. D. R.; DANTAS, K. das G. F.; CARVALHO, F. I. M.; GONÇALVES, D. de A.; SILVA, A. R.; SUN, H.; FERREIRA, M. J.; HEIN, P. R. G.; PROTÁSIO, T. de P. The inorganic composition of Tachigali vulgaris wood: implications for bioenergy and nutrient balances of planted forests in the Amazonia. BioEnergy Research, v. 17, p. 114-128, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 3 | |
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