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| Registros recuperados : 258 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
12/11/2020 |
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Data da última atualização: |
21/12/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVEIRA, S. S.; SANT'ANNA-SANTOS, B. F.; DEGENHARDT, J.; QUOIRIN, M. |
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Afiliação: |
Sheila Susy Silveira, UFPR; Bruno Francisco Sant'Anna-Santos, UFPR; JULIANA DEGENHARDT, CNPF; Marguerite Quoirin, UFPR. |
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Título: |
Somatic embryogenesis from mature split seeds of jaboticaba (Plinia peruviana (Poir) Govaerts). |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Acta Scientiarum. Agronomy, v. 42, e43798, 2020 11 p. |
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DOI: |
10.4025/actasciagron.v42i1.43798 |
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Idioma: |
Inglês |
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Conteúdo: |
Plinia peruviana is a species that is native to Brazil and is important due to the taste and medicinal properties of its fruits. Young leaves and split mature seeds were used as explants to initiate somatic embryogenesis to obtain a large number of plants in a short period of time. Leaf discs were cultured in MS medium containing various concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid) or picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid). In the case of the mature seeds, various concentrations of glutamine, 2,4-D and a combination of auxin and BAP (6-benzylaminopurine) were tested for somatic embryogenesis induction. For somatic embryo maturation, several concentrations of PEG 6000 (polyethylene glycol; up to 90 g L-1) were tested. After 60 days of culture using leaf discs, callus formation occurred in all treatments, with the highest averages obtained with 10 μM 2,4-D. However, these calluses did not form somatic embryos. For the cultured seeds, the best treatment was the MS medium with 1,000 mg L-1 glutamine and 10 μM 2,4-D without BAP. The supplementation of 60 g L-1 PEG 6000 was sufficient to promote the maturation of the somatic embryos. Histological analyses of the calluses that were formed from leaf discs showed nonembryogenic characteristics. In contrast, the calluses that originated from mature seeds had small and round cells with little vacuolation, which are characteristics of embryogenic structures. |
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Palavras-Chave: |
Análise histológica; Dichlorophenoxyacetic acid; Discos foliares; Histological analysis; Leaf discs; Massa pró-embriogênica; Mature seeds; Plinia peruviana; Proembryogenic masses; Semente madura. |
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Thesagro: |
Jabuticaba. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02390naa a2200301 a 4500
001 2126530
005 2021-12-21
008 2020 bl uuuu u00u1 u #d
024 7 $a10.4025/actasciagron.v42i1.43798$2DOI
100 1 $aSILVEIRA, S. S.
245 $aSomatic embryogenesis from mature split seeds of jaboticaba (Plinia peruviana (Poir) Govaerts).$h[electronic resource]
260 $c2020
520 $aPlinia peruviana is a species that is native to Brazil and is important due to the taste and medicinal properties of its fruits. Young leaves and split mature seeds were used as explants to initiate somatic embryogenesis to obtain a large number of plants in a short period of time. Leaf discs were cultured in MS medium containing various concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid) or picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid). In the case of the mature seeds, various concentrations of glutamine, 2,4-D and a combination of auxin and BAP (6-benzylaminopurine) were tested for somatic embryogenesis induction. For somatic embryo maturation, several concentrations of PEG 6000 (polyethylene glycol; up to 90 g L-1) were tested. After 60 days of culture using leaf discs, callus formation occurred in all treatments, with the highest averages obtained with 10 μM 2,4-D. However, these calluses did not form somatic embryos. For the cultured seeds, the best treatment was the MS medium with 1,000 mg L-1 glutamine and 10 μM 2,4-D without BAP. The supplementation of 60 g L-1 PEG 6000 was sufficient to promote the maturation of the somatic embryos. Histological analyses of the calluses that were formed from leaf discs showed nonembryogenic characteristics. In contrast, the calluses that originated from mature seeds had small and round cells with little vacuolation, which are characteristics of embryogenic structures.
650 $aJabuticaba
653 $aAnálise histológica
653 $aDichlorophenoxyacetic acid
653 $aDiscos foliares
653 $aHistological analysis
653 $aLeaf discs
653 $aMassa pró-embriogênica
653 $aMature seeds
653 $aPlinia peruviana
653 $aProembryogenic masses
653 $aSemente madura
700 1 $aSANT'ANNA-SANTOS, B. F.
700 1 $aDEGENHARDT, J.
700 1 $aQUOIRIN, M.
773 $tActa Scientiarum. Agronomy$gv. 42, e43798, 2020 11 p.
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