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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sul. Para informações adicionais entre em contato com cppsul.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
20/11/2009 |
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Data da última atualização: |
23/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BENAVIDES, M. V.; WEIMER, T. A.; BORBA, M. F. S.; BERNE, M. E. A.; SACCO, A. M. S. |
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Afiliação: |
MAGDA VIEIRA BENAVIDES, CPPSUL; T. A. Weimer, ULBRA; MARCOS FLAVIO SILVA BORBA, CPPSUL; M. E. A. Berne, UFPEL; ANA MARIA SASTRE SACCO, CPPSUL. |
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Título: |
Genetic analyses of polymorphisms on ovine chromosomes 5 and 20 and their effect on resistance to internal parasites. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Small Ruminant Research, Amsterdam, v. 83, n.1-3, p. 67-73, May 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
A trial was conducted to evaluate the association between polymorphisms in, or close to, candidate genes and faecal egg counts (FEC) in sheep. Corriedale and Polwarth weaned ewe lambs from commercial flocks were submitted to three successive experimental challenges in naturally contaminated pastures in Southern Brazil. During the trial, Haemonchus contortus was the predominant parasite species. Individuals were phenotyped according to their post-challenge FEC averages. Polymorphisms at interleukin genes (IL-3, IL-4 and IL-5), located at sheep chromosome 5 (OAR5) and the microsatellite markers BM1815, CSRD226, OarHH56, and OMHCI, located at sheep chromosome 20 (OAR20), were tested. A forced PCR-RFLP method to genotype ovine IL-5 was also developed and described. Associations between phenotypes and genotypes were tested by allele substitution analyses. There was no significant association between logFEC and the polymorphisms examined here, except for IL-4 in the Corriedale population. Results showed that FEC averages were significantly reducedwhen IL-4*B allele, themost frequent allele,was replaced by either IL-4*A or IL-4*C alleles (P < 0.05). |
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Palavras-Chave: |
Parasito intestinal. |
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Thesagro: |
Marcador molecular; Ovino; Parasito. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01819naa a2200217 a 4500
001 1575580
005 2019-10-23
008 2009 bl uuuu u00u1 u #d
100 1 $aBENAVIDES, M. V.
245 $aGenetic analyses of polymorphisms on ovine chromosomes 5 and 20 and their effect on resistance to internal parasites.
260 $c2009
520 $aA trial was conducted to evaluate the association between polymorphisms in, or close to, candidate genes and faecal egg counts (FEC) in sheep. Corriedale and Polwarth weaned ewe lambs from commercial flocks were submitted to three successive experimental challenges in naturally contaminated pastures in Southern Brazil. During the trial, Haemonchus contortus was the predominant parasite species. Individuals were phenotyped according to their post-challenge FEC averages. Polymorphisms at interleukin genes (IL-3, IL-4 and IL-5), located at sheep chromosome 5 (OAR5) and the microsatellite markers BM1815, CSRD226, OarHH56, and OMHCI, located at sheep chromosome 20 (OAR20), were tested. A forced PCR-RFLP method to genotype ovine IL-5 was also developed and described. Associations between phenotypes and genotypes were tested by allele substitution analyses. There was no significant association between logFEC and the polymorphisms examined here, except for IL-4 in the Corriedale population. Results showed that FEC averages were significantly reducedwhen IL-4*B allele, themost frequent allele,was replaced by either IL-4*A or IL-4*C alleles (P < 0.05).
650 $aMarcador molecular
650 $aOvino
650 $aParasito
653 $aParasito intestinal
700 1 $aWEIMER, T. A.
700 1 $aBORBA, M. F. S.
700 1 $aBERNE, M. E. A.
700 1 $aSACCO, A. M. S.
773 $tSmall Ruminant Research, Amsterdam$gv. 83, n.1-3, p. 67-73, May 2009.
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