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Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
27/09/2012 |
Data da última atualização: |
09/01/2013 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
SILVA, A. P.; BABUJIA, L. C.; MATSUMOTO, L. S.; GUIMARÃES, M. de F.; HUNGRIA, M. |
Afiliação: |
ADRIANA PEREIRA DA SILVA, UEL; LETÍCIA CARLOS BABUJIA, UEM; LEOPOLDO SUSSUMU MATSUMOTO, UENP; MARIA DE FÁTIMA GUIMARÃES, UEL; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
Título: |
Bacterial diversity under different tillage and crop rotation systems. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa: SBCS, 2012. 4 p. Trab. 1752. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Notas: |
Fertbio. |
Conteúdo: |
Microbial diversity can be used to assess the impact of agricultural practices on the long-term sustainability of cropping systems. The aim of this study was to investigate changes in soil bacterial diversity as a result of the impact of different soil tillage and crop rotation systems. Bacterial diversity was examined in the 0-10 cm layer in two field experiments by analyzing soil DNA using 16S rDNA PCR-DGGE profiles. Experiment 1 was a 26-year trial consisting of four soil tillage management systems: (1) no-tillage (NT), (2) disc plow (DP), (3) field cultivator (FC) and (4) heavy-disc harrow (DH), all under soybean(summer)/wheat(winter) crop succession. Experiment 2 was a 10-year trial consisting of DP and NT and three crop rotations (CR) including grasses, legumes and green manures. Cluster analysis of the 16S rDNA sequences revealed that the main effect on clustering was attributed to differences in soil tillage management systems. The Shannon index confirmed greater bacterial diversity under NT, followed by the FC, DH and DP. Therefore, diversity decreased as tillage practices intensified. The evenness index demonstrated the uniformity of the profiles of the bacterial communities with dominance of a few communities, regardless of soil tillage and crop rotation. Different crop rotations had only minor effects on bacterial diversity, but the results could be related to a previous fallow period. The results suggest that the DGGE profiles are sensitive bioindicators of the changes in bacterial diversity caused by soil tillage. MenosMicrobial diversity can be used to assess the impact of agricultural practices on the long-term sustainability of cropping systems. The aim of this study was to investigate changes in soil bacterial diversity as a result of the impact of different soil tillage and crop rotation systems. Bacterial diversity was examined in the 0-10 cm layer in two field experiments by analyzing soil DNA using 16S rDNA PCR-DGGE profiles. Experiment 1 was a 26-year trial consisting of four soil tillage management systems: (1) no-tillage (NT), (2) disc plow (DP), (3) field cultivator (FC) and (4) heavy-disc harrow (DH), all under soybean(summer)/wheat(winter) crop succession. Experiment 2 was a 10-year trial consisting of DP and NT and three crop rotations (CR) including grasses, legumes and green manures. Cluster analysis of the 16S rDNA sequences revealed that the main effect on clustering was attributed to differences in soil tillage management systems. The Shannon index confirmed greater bacterial diversity under NT, followed by the FC, DH and DP. Therefore, diversity decreased as tillage practices intensified. The evenness index demonstrated the uniformity of the profiles of the bacterial communities with dominance of a few communities, regardless of soil tillage and crop rotation. Different crop rotations had only minor effects on bacterial diversity, but the results could be related to a previous fallow period. The results suggest that the DGGE profiles are sensitive bioindicators of the ... Mostrar Tudo |
Thesagro: |
Rotação de cultura; Sistema de cultivo. |
Thesaurus Nal: |
Crop rotation. |
Categoria do assunto: |
A Sistemas de Cultivo |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/66926/1/FERTBIO-1752.pdf
|
Marc: |
LEADER 02486nam a2200217 a 4500 001 1934761 005 2013-01-09 008 2012 bl uuuu u00u1 u #d 100 1 $aSILVA, A. P. 245 $aBacterial diversity under different tillage and crop rotation systems. 260 $aIn: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 30.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 14.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 12.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 9.; SIMPÓSIO SOBRE SELÊNIO NO BRASIL, 1., 2012, Maceió. A responsabilidade socioambiental da pesquisa agrícola: anais. Viçosa: SBCS, 2012. 4 p. Trab. 1752.$c2012 300 $c1 CD-ROM. 500 $aFertbio. 520 $aMicrobial diversity can be used to assess the impact of agricultural practices on the long-term sustainability of cropping systems. The aim of this study was to investigate changes in soil bacterial diversity as a result of the impact of different soil tillage and crop rotation systems. Bacterial diversity was examined in the 0-10 cm layer in two field experiments by analyzing soil DNA using 16S rDNA PCR-DGGE profiles. Experiment 1 was a 26-year trial consisting of four soil tillage management systems: (1) no-tillage (NT), (2) disc plow (DP), (3) field cultivator (FC) and (4) heavy-disc harrow (DH), all under soybean(summer)/wheat(winter) crop succession. Experiment 2 was a 10-year trial consisting of DP and NT and three crop rotations (CR) including grasses, legumes and green manures. Cluster analysis of the 16S rDNA sequences revealed that the main effect on clustering was attributed to differences in soil tillage management systems. The Shannon index confirmed greater bacterial diversity under NT, followed by the FC, DH and DP. Therefore, diversity decreased as tillage practices intensified. The evenness index demonstrated the uniformity of the profiles of the bacterial communities with dominance of a few communities, regardless of soil tillage and crop rotation. Different crop rotations had only minor effects on bacterial diversity, but the results could be related to a previous fallow period. The results suggest that the DGGE profiles are sensitive bioindicators of the changes in bacterial diversity caused by soil tillage. 650 $aCrop rotation 650 $aRotação de cultura 650 $aSistema de cultivo 700 1 $aBABUJIA, L. C. 700 1 $aMATSUMOTO, L. S. 700 1 $aGUIMARÃES, M. de F. 700 1 $aHUNGRIA, M.
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Registro Completo
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
18/08/2020 |
Data da última atualização: |
10/09/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
TEIXEIRA, W. F. A.; BATISTA, R. D.; SANTOS, C. C. A. do A.; FREITAS JÚNIOR, A. C. F.; TERRASAN, C. R. F.; SANTANA, M. W. P. R. de; SIQUEIRA, F. G. de; PAULA-ELIAS, F. C. de; ALMEIDA, A. F. de. |
Afiliação: |
Wéllen Fabrícia Aviz Teixeira, Universidade Federal do Tocantins; Ryhára Dias Batista, Universidade Federal do Tocantins; Claudia Cristina Auler do Amaral Santos, Universidade Federal do Tocantins; Aloísio Chagas Freitas Júnior, Universidade Federal do Tocantins; César Rafael Fanchini Terrasan, Universidade Estadual Paulista; Marcio Waluce Pinheiro R. de Santana, Universidade Federal da Bahia; FELIX GONCALVES DE SIQUEIRA, CNPAE; Fabrício Coutinho de Paula-Elias, Universidade Federal do Tocantins; Alex Fernando de Almeida, Universidade Federal do Tocantins. |
Título: |
Minimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Waste Biomass Valorization, 2020. |
ISSN: |
1877-265X |
DOI: |
https://doi.org/10.1007/s12649-020-01199-8 |
Idioma: |
Inglês |
Notas: |
On-line first. Published online: 11 August 2020. |
Conteúdo: |
Agroindustrial wastes is an interesting opportunity for enzymes production, reducing operational costs and strengthening the biorefineries concept. The conversion of lignocellulosic waste to fermentable sugars is current challenge for biorefineries industries. Enzyme cocktail used in delignification and saccharification platform are produced by a combinatory of several lignocellulolytic enzymes. The aim of this study was to develop filamentous fungi compatible-consortia isolated from pineapple wastes for cellulose-degrading enzymes production using pineapple crown waste in solid-state cultivation and its potential for saccharification of pineapple crown waste. Isolates were screened in agar-plate and in solid-state cultivation for cellulases, xylanase and pectinases productions. Six compatible consortia of Trichoderma strains with Aspergillus niger or Pleurotus ostreatus were evaluated for SSC using pineapple crown. All consortia increased the enzymes production compared to monoculture. Consortia of Trichoderma asperellum PEC-6 and P. ostreatus increased 1.60-fold the xylanase and 1.42-fold the Beta-glucosidase productions. T. asperellum PEC-17 and A. niger increased 1.16-fold endoglucanase and 1.28-fold pectinase. Saccharification of pineapple crown waste using enzyme cocktail produced by filamentous fungi consortia increased the total reducing sugar released in 12.50–13.93% compared to enzymes produced by monocultures. This study provides an alternative model to the cocktail enzyme production by mixed cultures development with lower cost of on-site enzyme manufacture by use of agroindustrial wastes eliminating an enzyme blended step. Moreover, the procedure used in this work can be potentially cost saving and environmentally friendly and should be explored on other bioenergy feedstocks and feed production. MenosAgroindustrial wastes is an interesting opportunity for enzymes production, reducing operational costs and strengthening the biorefineries concept. The conversion of lignocellulosic waste to fermentable sugars is current challenge for biorefineries industries. Enzyme cocktail used in delignification and saccharification platform are produced by a combinatory of several lignocellulolytic enzymes. The aim of this study was to develop filamentous fungi compatible-consortia isolated from pineapple wastes for cellulose-degrading enzymes production using pineapple crown waste in solid-state cultivation and its potential for saccharification of pineapple crown waste. Isolates were screened in agar-plate and in solid-state cultivation for cellulases, xylanase and pectinases productions. Six compatible consortia of Trichoderma strains with Aspergillus niger or Pleurotus ostreatus were evaluated for SSC using pineapple crown. All consortia increased the enzymes production compared to monoculture. Consortia of Trichoderma asperellum PEC-6 and P. ostreatus increased 1.60-fold the xylanase and 1.42-fold the Beta-glucosidase productions. T. asperellum PEC-17 and A. niger increased 1.16-fold endoglucanase and 1.28-fold pectinase. Saccharification of pineapple crown waste using enzyme cocktail produced by filamentous fungi consortia increased the total reducing sugar released in 12.50–13.93% compared to enzymes produced by monocultures. This study provides an alternative model to the cockta... Mostrar Tudo |
Palavras-Chave: |
Biomass saccharification; Filamentous Fungi Isolation; Microbial consortia; Resíduos lignocelulósicos; Solid-state cultivation. |
Thesagro: |
Abacaxi; Bioenergia; Biomassa; Enzima; Resíduo Industrial; Trichoderma. |
Thesaurus NAL: |
Agricultural wastes; Aspergillus; Bioenergy industry; Biomass; Enzymes; Industrial wastes; Lignocellulosic wastes; Microbial culture; Pineapples; Trichoderma asperellum. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03430naa a2200505 a 4500 001 2124402 005 2020-09-10 008 2020 bl uuuu u00u1 u #d 022 $a1877-265X 024 7 $ahttps://doi.org/10.1007/s12649-020-01199-8$2DOI 100 1 $aTEIXEIRA, W. F. A. 245 $aMinimal Enzymes Cocktail Development by Filamentous Fungi Consortia in Solid-State Cultivation and Valorization of Pineapple Crown Waste by Enzymatic Saccharification.$h[electronic resource] 260 $c2020 500 $aOn-line first. Published online: 11 August 2020. 520 $aAgroindustrial wastes is an interesting opportunity for enzymes production, reducing operational costs and strengthening the biorefineries concept. The conversion of lignocellulosic waste to fermentable sugars is current challenge for biorefineries industries. Enzyme cocktail used in delignification and saccharification platform are produced by a combinatory of several lignocellulolytic enzymes. The aim of this study was to develop filamentous fungi compatible-consortia isolated from pineapple wastes for cellulose-degrading enzymes production using pineapple crown waste in solid-state cultivation and its potential for saccharification of pineapple crown waste. Isolates were screened in agar-plate and in solid-state cultivation for cellulases, xylanase and pectinases productions. Six compatible consortia of Trichoderma strains with Aspergillus niger or Pleurotus ostreatus were evaluated for SSC using pineapple crown. All consortia increased the enzymes production compared to monoculture. Consortia of Trichoderma asperellum PEC-6 and P. ostreatus increased 1.60-fold the xylanase and 1.42-fold the Beta-glucosidase productions. T. asperellum PEC-17 and A. niger increased 1.16-fold endoglucanase and 1.28-fold pectinase. Saccharification of pineapple crown waste using enzyme cocktail produced by filamentous fungi consortia increased the total reducing sugar released in 12.50–13.93% compared to enzymes produced by monocultures. This study provides an alternative model to the cocktail enzyme production by mixed cultures development with lower cost of on-site enzyme manufacture by use of agroindustrial wastes eliminating an enzyme blended step. Moreover, the procedure used in this work can be potentially cost saving and environmentally friendly and should be explored on other bioenergy feedstocks and feed production. 650 $aAgricultural wastes 650 $aAspergillus 650 $aBioenergy industry 650 $aBiomass 650 $aEnzymes 650 $aIndustrial wastes 650 $aLignocellulosic wastes 650 $aMicrobial culture 650 $aPineapples 650 $aTrichoderma asperellum 650 $aAbacaxi 650 $aBioenergia 650 $aBiomassa 650 $aEnzima 650 $aResíduo Industrial 650 $aTrichoderma 653 $aBiomass saccharification 653 $aFilamentous Fungi Isolation 653 $aMicrobial consortia 653 $aResíduos lignocelulósicos 653 $aSolid-state cultivation 700 1 $aBATISTA, R. D. 700 1 $aSANTOS, C. C. A. do A. 700 1 $aFREITAS JÚNIOR, A. C. F. 700 1 $aTERRASAN, C. R. F. 700 1 $aSANTANA, M. W. P. R. de 700 1 $aSIQUEIRA, F. G. de 700 1 $aPAULA-ELIAS, F. C. de 700 1 $aALMEIDA, A. F. de 773 $tWaste Biomass Valorization, 2020.
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