Data/hora: 13/06/2024 14:40:56

Biblioteca(s): | Embrapa Agricultura Digital.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | Internacional - A
Data corrente: | 16/02/2009
Data da última atualização: | 15/01/2020
Autoria: | BALAN, A.; SANTACRUZ-PÉREZ, C.; MOUTRAN, A.; FERREIRA, L. C. S.; NESHICH, G.; BARBOSA, J. A. R. G.
Afiliação: | ANDREA BALAN, USP; CAROLINA SANTACRUZ-PÉREZ, LNLS; ALEXANDRE MOUTRAN, USP; LUÍS CARLOS SOUZA FERREIRA, USP; GORAN NESHICH, CNPTIA; JOÃO ALEXANDRE RIBEIRO GONÇALVES BARBOSA, LNLS.
Título: | Crystallographic structure and substrate-binding interactions of the molybdate-binding protein of the phytopathogen Xanthomonas axonopodis pv. citri.
Ano de publicação: | 2008
Fonte/Imprenta: | Biochimica et Biophysica Acta, v. 1784, n. 2, p. 393-399, 2008.
DOI: | 10.1016/j.bbapap.2007.11.013
Idioma: | Inglês
Conteúdo: | In Xanthomonas axonopodis pv. citri (Xac or X. citri), the modA gene codes for a periplasmic protein (ModA) that is capable of binding molybdate and tungstate as part of the ABC-type transporter required for the uptake of micronutrients. In this study, we report the crystallographic structure of the Xac ModA protein with bound molybdate. The Xac ModA structure is similar to orthologs with known three-dimensional structures and consists of two nearly symmetrical domains separated by a hinge region where the oxyanion-binding site lies. Phylogenetic analysis of different ModA orthologs based on sequence alignments revealed three groups of molybdate-binding proteins: bacterial phytopathogens, enterobacteria and soil bacteria. Even though the ModA orthologs are segregated into different groups, the ligand-binding hydrogen bonds are mostly conserved, except for Archaeglobus fulgidus ModA. A detailed discussion of hydrophobic interactions in the active site is presented and two new residues, Ala38 and Ser151, are shown to be part of the ligand-binding pocket.
Palavras-Chave: | Estrutura de cristalografia de raio-X; ModA; Molybdate-binding protein; X-ray crystal structure.
Thesagro: | Proteína.
Thesaurus Nal: | Xanthomonas axonopodis.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Agricultura Digital (CNPTIA)

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