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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
23/06/2017 |
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Data da última atualização: |
29/06/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KMIT, M. C. P.; LIMA, A. O. S.; FREITAS, R. C.; ROMAGNOLI, E. M.; ABDALLA, A. L.; MENDES, R. |
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Afiliação: |
M. C. P. KMIT, ESALQ/USP; A. O. S. LIMA, University of Vale do Itajaí; R. C. FREITAS, University of Vale do Itajaí; E. M. ROMAGNOLI, ESALQ/USP; A. L. ABDALLA, CENA, ESALQ/USP; RODRIGO MENDES, CNPMA. |
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Título: |
Exploring the sheep rumen shotgun sequencing for funcional analysis and lignocellulolitic enzyme discovery. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: CONGRESO LATINOAMERICANO DE MICROBIOLOGÍA, 23.; CONGRESO ARGENTINO DE MICROBIOLOGIA, 14.; 2016, Rosario. Libro de Resumenes... Rosario: Asociación Latinoamericana de Microbiología; Asociación Argentina de Microbiología, 2016. Ref. JU-1377. |
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Idioma: |
Inglês |
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Conteúdo: |
The rumen harbors complex microbial communities which participate in an efficient process to digest plant biomass. This ecosytem represents an untapped source of hydrolytic enzymes with potential application for second?generation biofuel production from lignocellulosic biomass. The search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to describe the functions and explores the potential for lignocellulolitic enzyme in the sheep rumen microbiome. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. Metagenomic DNA was extracted from the solid rumen contents and sequencing was performed in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 GB of metagenomic DNA from microbes adherent to plant fiber using MGRAST metagenomics analysis server. The functional annotation was performed at MG?RAST for the total functional profile using the KEGG orthology level 2. The shotgun metagenomic reads of all animals samples was assigned to putative lignocellulolitic enzymes when considering nine protein databases at MG?RAST. The predictive functional profiling of the sheep rumen microbiome revealed that amino acid and carbohydrate metabolism, translation, DNA replication and repair, and membrane transport are dominant functions in the rumen microbiome. This functional pattern was similar across all animals. As expected, carbohydrate metabolism was highly represented in our data set, supporting the importance of the rumen microbiome for fiber degradation. Reads classification using nine databases resulted in 22 lignocellulases. For instance, the TrEMBL representing 76,77% out of a total 933639 protein abundance, followed by SwissProt representing 39,95 %, Seed 20,71%, PATRIC 5,92%, IMG 3,63%, KEEG 3,44%, GenBank 3,13%, RefSeq 3,09%, eggnog 1,48%. Based on Cazy search for glycosyl hydrolase (GH) families, more than 50 GH families were detected . The most abundant enzymes were ??glucosidase (GH1; GH30), Endo?1,4???xylanase (GH5; GH10; GH51) , ??N?arabinofuranosidase (GH7; GH51; GH54), ??galactosidase( GH27; GH31; GH36), Acetylesterase, Cellulase (GH5; GH9, GH7), Cellobiose phosphorylase (GH94), ??mannosidase(GH2; GH5), ??galactosidase(GH1; GH2; GH35). This results showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes. MenosThe rumen harbors complex microbial communities which participate in an efficient process to digest plant biomass. This ecosytem represents an untapped source of hydrolytic enzymes with potential application for second?generation biofuel production from lignocellulosic biomass. The search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to describe the functions and explores the potential for lignocellulolitic enzyme in the sheep rumen microbiome. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. Metagenomic DNA was extracted from the solid rumen contents and sequencing was performed in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 GB of metagenomic DNA from microbes adherent to plant fiber using MGRAST metagenomics analysis server. The functional annotation was performed at MG?RAST for the total functional profile using the KEGG orthology level 2. The shotgun metagenomic reads of all animals samples was assigned to putative lignocellulolitic enzymes when considering nine protein databases at MG?RAST. The predictive functional profiling of the sheep rumen microbiome revealed that amino acid... Mostrar Tudo |
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Thesagro: |
Enzima; Rúmen. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161026/1/2016RA-086.pdf
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Marc: |
LEADER 03439nam a2200193 a 4500
001 2071445
005 2017-06-29
008 2016 bl uuuu u00u1 u #d
100 1 $aKMIT, M. C. P.
245 $aExploring the sheep rumen shotgun sequencing for funcional analysis and lignocellulolitic enzyme discovery.$h[electronic resource]
260 $aIn: CONGRESO LATINOAMERICANO DE MICROBIOLOGÍA, 23.; CONGRESO ARGENTINO DE MICROBIOLOGIA, 14.; 2016, Rosario. Libro de Resumenes... Rosario: Asociación Latinoamericana de Microbiología; Asociación Argentina de Microbiología, 2016. Ref. JU-1377.$c1377
520 $aThe rumen harbors complex microbial communities which participate in an efficient process to digest plant biomass. This ecosytem represents an untapped source of hydrolytic enzymes with potential application for second?generation biofuel production from lignocellulosic biomass. The search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to describe the functions and explores the potential for lignocellulolitic enzyme in the sheep rumen microbiome. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. Metagenomic DNA was extracted from the solid rumen contents and sequencing was performed in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 GB of metagenomic DNA from microbes adherent to plant fiber using MGRAST metagenomics analysis server. The functional annotation was performed at MG?RAST for the total functional profile using the KEGG orthology level 2. The shotgun metagenomic reads of all animals samples was assigned to putative lignocellulolitic enzymes when considering nine protein databases at MG?RAST. The predictive functional profiling of the sheep rumen microbiome revealed that amino acid and carbohydrate metabolism, translation, DNA replication and repair, and membrane transport are dominant functions in the rumen microbiome. This functional pattern was similar across all animals. As expected, carbohydrate metabolism was highly represented in our data set, supporting the importance of the rumen microbiome for fiber degradation. Reads classification using nine databases resulted in 22 lignocellulases. For instance, the TrEMBL representing 76,77% out of a total 933639 protein abundance, followed by SwissProt representing 39,95 %, Seed 20,71%, PATRIC 5,92%, IMG 3,63%, KEEG 3,44%, GenBank 3,13%, RefSeq 3,09%, eggnog 1,48%. Based on Cazy search for glycosyl hydrolase (GH) families, more than 50 GH families were detected . The most abundant enzymes were ??glucosidase (GH1; GH30), Endo?1,4???xylanase (GH5; GH10; GH51) , ??N?arabinofuranosidase (GH7; GH51; GH54), ??galactosidase( GH27; GH31; GH36), Acetylesterase, Cellulase (GH5; GH9, GH7), Cellobiose phosphorylase (GH94), ??mannosidase(GH2; GH5), ??galactosidase(GH1; GH2; GH35). This results showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes.
650 $aEnzima
650 $aRúmen
700 1 $aLIMA, A. O. S.
700 1 $aFREITAS, R. C.
700 1 $aROMAGNOLI, E. M.
700 1 $aABDALLA, A. L.
700 1 $aMENDES, R.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
30/01/2018 |
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Data da última atualização: |
28/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
TORRES JÚNIOR, C. V.; ARAUJO, K. E. C.; ALVES, L. S.; SOUZA, S. R. de; SANTOS, L. A.; SANTA-CATARINA, C.; SILVA, K. da; PEREIRA, G. M. D.; XAVIER, G. R.; ZILLI, J. E. |
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Afiliação: |
CARLOS VERGARA TORRES JUNIOR, UFRRJ; KARLA EMANUELLE CAMPOS ARAUJO, UFRRJ; LUIZIENE SOARES ALVES, UFRRJ; SÔNIA REGINA DE SOUZA, UFRRJ; LEANDRO AZEVEDO SANTOS, UFRRJ; CLAUDETE SANTA-CATARINA, UENF; KRISLE DA SILVA, CNPF; GILMARA MARIA DUARTE PEREIRA, UFRR; GUSTAVO RIBEIRO XAVIER, CNPAB; JERRI EDSON ZILLI, CNPAB. |
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Título: |
Contribution of dark septate fungi to the nutrient uptake and growth of rice plants |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, Environmental Microbiology, v. 49, n. 1, p. 67-78, jan./mar. 2018. |
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DOI: |
https://doi.org/10.1016/j.bjm.2017.04.010 |
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Idioma: |
Inglês |
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Conteúdo: |
The use of dark septate fungi (DSE) to promote plant growth can be beneficial to agriculture, and these organisms are important allies in the search for sustainable agriculture practices. This study investigates the contribution of dark septate fungi to the absorption of nutrients by rice plants and their ensuing growth. Four dark septate fungi isolates that were identified by Internal transcribed spacer phylogeny were inoculated in rice seeds (Cv. Piauí). The resulting root colonization was estimated and the kinetic parameters Vmax and Km were calculated from the nitrate contents of the nutrient solution. The macronutrient levels in the shoots, and the NO3?-N, NH4+-N, free amino-N and soluble sugars in the roots, sheathes and leaves were measured. The rice roots were significantly colonized by all of the fungi, but in particular, isolate A103 increased the fresh and dry biomass of the shoots and the number of tillers per plant, amino-N, and soluble sugars as well as the N, P, K, Mg and S contents in comparison with the control treatment. When inoculated with isolates A103 and A101, the plants presented lower Km values, indicating affinity increases for NO3?-N absorption. Therefore, the A103 Pleosporales fungus presented the highest potential for the promotion of rice plant growth, increasing the tillering and nutrients uptake, especially N (due to an enhanced affinity for N uptake) and P. |
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Palavras-Chave: |
Colonization; Oryza sativa L. |
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Thesaurus NAL: |
tillering. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02282naa a2200277 a 4500
001 2086741
005 2023-08-28
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.bjm.2017.04.010$2DOI
100 1 $aTORRES JÚNIOR, C. V.
245 $aContribution of dark septate fungi to the nutrient uptake and growth of rice plants$h[electronic resource]
260 $c2018
520 $aThe use of dark septate fungi (DSE) to promote plant growth can be beneficial to agriculture, and these organisms are important allies in the search for sustainable agriculture practices. This study investigates the contribution of dark septate fungi to the absorption of nutrients by rice plants and their ensuing growth. Four dark septate fungi isolates that were identified by Internal transcribed spacer phylogeny were inoculated in rice seeds (Cv. Piauí). The resulting root colonization was estimated and the kinetic parameters Vmax and Km were calculated from the nitrate contents of the nutrient solution. The macronutrient levels in the shoots, and the NO3?-N, NH4+-N, free amino-N and soluble sugars in the roots, sheathes and leaves were measured. The rice roots were significantly colonized by all of the fungi, but in particular, isolate A103 increased the fresh and dry biomass of the shoots and the number of tillers per plant, amino-N, and soluble sugars as well as the N, P, K, Mg and S contents in comparison with the control treatment. When inoculated with isolates A103 and A101, the plants presented lower Km values, indicating affinity increases for NO3?-N absorption. Therefore, the A103 Pleosporales fungus presented the highest potential for the promotion of rice plant growth, increasing the tillering and nutrients uptake, especially N (due to an enhanced affinity for N uptake) and P.
650 $atillering
653 $aColonization
653 $aOryza sativa L
700 1 $aARAUJO, K. E. C.
700 1 $aALVES, L. S.
700 1 $aSOUZA, S. R. de
700 1 $aSANTOS, L. A.
700 1 $aSANTA-CATARINA, C.
700 1 $aSILVA, K. da
700 1 $aPEREIRA, G. M. D.
700 1 $aXAVIER, G. R.
700 1 $aZILLI, J. E.
773 $tBrazilian Journal of Microbiology, Environmental Microbiology$gv. 49, n. 1, p. 67-78, jan./mar. 2018.
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Embrapa Agrobiologia (CNPAB) |
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