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| Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
11/05/2020 |
Data da última atualização: |
15/07/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CARMO, C. D. do; SOUSA, M. B. e; SILVA, P. P. dos S.; OLIVEIRA, G. A. F.; CEBALLOS, H.; OLIVEIRA, E. J. de. |
Afiliação: |
CÁTIA DIAS DO CARMO; MASSAINE BANDEIRA E SOUSA; PRISCILA PATRÍCIA DOS SANTOS SILVA; GILMARA ALVARENGA FACHARDO OLIVEIRA; HERNÁN CEBALLOS; EDER JORGE DE OLIVEIRA, CNPMF. |
Título: |
Identification and validation of mutation points associated with waxy phenotype in cassava. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
BMC Plant Biology, v. 20, p.164, 2020. |
ISSN: |
1471-2229 |
Idioma: |
Inglês |
Conteúdo: |
BACKGROUND: The granule-bound starch synthase I (GBSSI) enzyme is responsible for the synthesis of amylose, and therefore, its absence results in individuals with a waxy starch phenotype in various amylaceous crops. The validation of mutation points previously associated with the waxy starch phenotype in cassava, as well as the identification of alternative mutant alleles in the GBSSI gene, can allow the development of molecular-assisted selection to introgress the waxy starch mutation into cassava breeding populations. RESULTS: A waxy cassava allele has been identified previously, associated with several SNPs. A particular SNP (intron 11) was used to develop SNAP markers for screening heterozygote types in cassava germplasm. Although the molecular segregation corresponds to the expected segregation at 3:1 ratio (dominant gene for the presence of amylose), the homozygotes containing the SNP associated with the waxy mutation did not show waxy phenotypes. To identify more markers, we sequenced the GBSS gene from 89 genotypes, including some that were segregated from a cross with a line carrying the known waxy allele. As a result, 17 mutations in the GBSSI gene were identified, in which only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype. The evaluation of mutation points by discriminant analysis of principal component analysis (DAPC) also did not completely discriminate the waxy individuals. Therefore, we developed Kompetitive Allele Specific PCR (KASP) markers that allowed discrimination between WX and wx alleles. The results demonstrated the non-existence of heterozygous individuals of the MeWxEx6-del-C deletion in the analyzed germplasm. Therefore, the deletion MeWxEx6-del-C should not be used for assisted selection in genetic backgrounds different from the original source of waxy starch. Also, the alternative SNPs identified in this study were not associated with the waxy phenotype when compared to a panel of accessions with high genetic diversity. CONCLUSION: Although the GBSSI gene can exhibit several mutations in cassava, only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype in the original AM206-5 source. MenosBACKGROUND: The granule-bound starch synthase I (GBSSI) enzyme is responsible for the synthesis of amylose, and therefore, its absence results in individuals with a waxy starch phenotype in various amylaceous crops. The validation of mutation points previously associated with the waxy starch phenotype in cassava, as well as the identification of alternative mutant alleles in the GBSSI gene, can allow the development of molecular-assisted selection to introgress the waxy starch mutation into cassava breeding populations. RESULTS: A waxy cassava allele has been identified previously, associated with several SNPs. A particular SNP (intron 11) was used to develop SNAP markers for screening heterozygote types in cassava germplasm. Although the molecular segregation corresponds to the expected segregation at 3:1 ratio (dominant gene for the presence of amylose), the homozygotes containing the SNP associated with the waxy mutation did not show waxy phenotypes. To identify more markers, we sequenced the GBSS gene from 89 genotypes, including some that were segregated from a cross with a line carrying the known waxy allele. As a result, 17 mutations in the GBSSI gene were identified, in which only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype. The evaluation of mutation points by discriminant analysis of principal component analysis (DAPC) also did not completely discriminate the waxy individuals. Therefore, we developed Kompetitive Allele Specific PCR... Mostrar Tudo |
Thesagro: |
Mandioca. |
Thesaurus Nal: |
Cassava. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02827naa a2200217 a 4500 001 2122191 005 2020-07-15 008 2020 bl uuuu u00u1 u #d 022 $a1471-2229 100 1 $aCARMO, C. D. do 245 $aIdentification and validation of mutation points associated with waxy phenotype in cassava.$h[electronic resource] 260 $c2020 520 $aBACKGROUND: The granule-bound starch synthase I (GBSSI) enzyme is responsible for the synthesis of amylose, and therefore, its absence results in individuals with a waxy starch phenotype in various amylaceous crops. The validation of mutation points previously associated with the waxy starch phenotype in cassava, as well as the identification of alternative mutant alleles in the GBSSI gene, can allow the development of molecular-assisted selection to introgress the waxy starch mutation into cassava breeding populations. RESULTS: A waxy cassava allele has been identified previously, associated with several SNPs. A particular SNP (intron 11) was used to develop SNAP markers for screening heterozygote types in cassava germplasm. Although the molecular segregation corresponds to the expected segregation at 3:1 ratio (dominant gene for the presence of amylose), the homozygotes containing the SNP associated with the waxy mutation did not show waxy phenotypes. To identify more markers, we sequenced the GBSS gene from 89 genotypes, including some that were segregated from a cross with a line carrying the known waxy allele. As a result, 17 mutations in the GBSSI gene were identified, in which only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype. The evaluation of mutation points by discriminant analysis of principal component analysis (DAPC) also did not completely discriminate the waxy individuals. Therefore, we developed Kompetitive Allele Specific PCR (KASP) markers that allowed discrimination between WX and wx alleles. The results demonstrated the non-existence of heterozygous individuals of the MeWxEx6-del-C deletion in the analyzed germplasm. Therefore, the deletion MeWxEx6-del-C should not be used for assisted selection in genetic backgrounds different from the original source of waxy starch. Also, the alternative SNPs identified in this study were not associated with the waxy phenotype when compared to a panel of accessions with high genetic diversity. CONCLUSION: Although the GBSSI gene can exhibit several mutations in cassava, only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype in the original AM206-5 source. 650 $aCassava 650 $aMandioca 700 1 $aSOUSA, M. B. e 700 1 $aSILVA, P. P. dos S. 700 1 $aOLIVEIRA, G. A. F. 700 1 $aCEBALLOS, H. 700 1 $aOLIVEIRA, E. J. de 773 $tBMC Plant Biology$gv. 20, p.164, 2020.
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| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
15/03/1993 |
Data da última atualização: |
15/03/1993 |
Autoria: |
RIBEIRO, J. F.; SANO, S. M.; SILVA, J. A. da. |
Título: |
Chave preliminar de identificacao dos tipos fisionomicos da vegetacao dos cerrados. |
Ano de publicação: |
1981 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE BOTANICA, 32., 1981, Teresina. Anais. Teresina: SBB/UFPI, 1981. |
Páginas: |
p.124-133. |
Idioma: |
Português |
Conteúdo: |
Varios autores ainda discutem a nomenclatura dos tipos fisionomicos ocorrentes na Regiao dos Cerrados. Apesar disso, nao se tem um consenso sobre uma padronizacao dessa monenclatura. Dada essa dificuldade, e proposta uma chave preliminar, com ilustracoes, para os tipos fisionomicos comumente diferenciados, visando facilitar a sua identificacao em trabalhos multidisciplinares. A partir de levantamento bibliografico e trabalho de campo foram identificados e separados os tipos fisionomicos de Mata de Galeria, mata Mesofitica, Cerradao, Cerrado, Campo Cerrado, Campo Sujo, Campo Limpo, Vereda, Campo Rupestre e Campo Murundu, os principais parametros usados na separacao dos tipos fisionomicos foram estrutura da vegetacao e caracteristicas do solo. Com relacao a estrutura da vegetacao foram considerados altura, estratificacao, caducifolia e porcentagem de cobertura arborea. Quanto as caracteristicas do solo foram utilizadas grau de umidade, profundidade e presenca ou nao de afloramento de rochas. |
Thesagro: |
Cerrado; Vegetação. |
Thesaurus NAL: |
vegetation. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01567naa a2200193 a 4500 001 1548296 005 1993-03-15 008 1981 bl --- 0-- u #d 100 1 $aRIBEIRO, J. F. 245 $aChave preliminar de identificacao dos tipos fisionomicos da vegetacao dos cerrados. 260 $c1981 300 $ap.124-133. 520 $aVarios autores ainda discutem a nomenclatura dos tipos fisionomicos ocorrentes na Regiao dos Cerrados. Apesar disso, nao se tem um consenso sobre uma padronizacao dessa monenclatura. Dada essa dificuldade, e proposta uma chave preliminar, com ilustracoes, para os tipos fisionomicos comumente diferenciados, visando facilitar a sua identificacao em trabalhos multidisciplinares. A partir de levantamento bibliografico e trabalho de campo foram identificados e separados os tipos fisionomicos de Mata de Galeria, mata Mesofitica, Cerradao, Cerrado, Campo Cerrado, Campo Sujo, Campo Limpo, Vereda, Campo Rupestre e Campo Murundu, os principais parametros usados na separacao dos tipos fisionomicos foram estrutura da vegetacao e caracteristicas do solo. Com relacao a estrutura da vegetacao foram considerados altura, estratificacao, caducifolia e porcentagem de cobertura arborea. Quanto as caracteristicas do solo foram utilizadas grau de umidade, profundidade e presenca ou nao de afloramento de rochas. 650 $avegetation 650 $aCerrado 650 $aVegetação 700 1 $aSANO, S. M. 700 1 $aSILVA, J. A. da 773 $tIn: CONGRESSO NACIONAL DE BOTANICA, 32., 1981, Teresina. Anais. Teresina: SBB/UFPI, 1981.
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