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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
14/12/2023 |
Data da última atualização: |
14/12/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
JORDÃO, L. S. B.; MORIM, M. P.; SIMON, M. F.; BAUMGRATZ, J. F. A. |
Afiliação: |
LUCAS SÁ BARRETO JORDÃO, Jardim Botânico do Rio de Janeiro; MARLI PIRES MORIM, Jardim Botânico do Rio de Janeiro; MARCELO FRAGOMENI SIMON, Cenargen; JOSÉ FERNANDO A. BAUMGRATZ, Jardim Botânico do Rio de Janeiro. |
Título: |
Towards a new systematics of Mimosa (Leguminosae): the Petiolovariabilis clade. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
In: INTERNATIONAL LEGUME CONFERENCE, 8., 2023, Pirenópolis. Integrating knowledge on the legume family: [abstracts]. Pirenópolis: [s.n.], 2023. |
Idioma: |
Inglês |
Palavras-Chave: |
Mimosoid; Seasonally dry vegetation. |
Thesaurus Nal: |
Phylogeny; Savannas; Taxonomy. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00673nam a2200193 a 4500 001 2159687 005 2023-12-14 008 2023 bl uuuu u00u1 u #d 100 1 $aJORDÃO, L. S. B. 245 $aTowards a new systematics of Mimosa (Leguminosae)$bthe Petiolovariabilis clade.$h[electronic resource] 260 $aIn: INTERNATIONAL LEGUME CONFERENCE, 8., 2023, Pirenópolis. Integrating knowledge on the legume family: [abstracts]. Pirenópolis: [s.n.]$c2023 650 $aPhylogeny 650 $aSavannas 650 $aTaxonomy 653 $aMimosoid 653 $aSeasonally dry vegetation 700 1 $aMORIM, M. P. 700 1 $aSIMON, M. F. 700 1 $aBAUMGRATZ, J. F. A.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
03/03/2015 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
DIAS, N. L.; FONSECA JÚNIOR, A. A.; OLIVEIRA, A. M.; SALES, É. B.; ALVES, B. R. C.; DORELLA, F. A.; CAMARGOS, M. F. |
Afiliação: |
NATANAEL LAMAS DIAS, LANAGRO - MG; ANTÔNIO AUGUSTO FONSECA JÚNIOR, LANAGRO - MG; ANAPOLINO MACEDO OLIVEIRA, LANAGRO - MG; ÉRICA BRAVO SALES, LANAGRO - MG; BRUNA RIOS COELHO ALVES, CNPGL; FERNANDA ALVES DORELLA, LANAGRO - MG; MARCELO FERNANDES CAMARGOS, LANAGRO - MG. |
Título: |
Validation of a real time PCR for classical swine fever diagnosis. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Veterinary Medicine International, 2014. |
Páginas: |
4 p. |
Idioma: |
Inglês |
Conteúdo: |
The viral disease classical swine fever (CSF), caused by a Pestivirus, is one of the major causes of economic losses for pig farming.The aim of this work was to validate a RT-qPCR using Taqman for detection of CSF in swine tissues. The parameters for the validation followed the specifications of the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organization for Animal Health (OIE) and the guide ABNT NBR ISO/IEC 17025:2005. The analysis of the 5' NTR region of CSF virus was performed in 145 samples from 29 infected pigs and in 240 samples from 80 pigs originated in the Brazilian CSF-free zone. The tissues tested were spleen, kidney, blood, tonsils, and lymph nodes. Sequencing of the positive samples for 5'NTR region was performed to evaluate the specificity of the RT-qPCR. Tests performed for the RT-qPCR validation demonstrated that the PCR assay was efficient in detecting RNA from CSF virus in all materials from different tissues of infected animals. Furthermore, RNA from CSF virus was not detected in samples of swine originated from the Brazilian CSF-free zone. Hence, it is concluded that RT-qPCR can be used as a complementary diagnostic for CSF. |
Palavras-Chave: |
Classical Swine Fever; PCR; Swine health. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119249/1/Cnpgl-2014-VetMedInt-Validation.pdf
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Marc: |
LEADER 01851naa a2200241 a 4500 001 2010526 005 2024-02-05 008 2014 bl uuuu u00u1 u #d 100 1 $aDIAS, N. L. 245 $aValidation of a real time PCR for classical swine fever diagnosis.$h[electronic resource] 260 $c2014 300 $a4 p. 520 $aThe viral disease classical swine fever (CSF), caused by a Pestivirus, is one of the major causes of economic losses for pig farming.The aim of this work was to validate a RT-qPCR using Taqman for detection of CSF in swine tissues. The parameters for the validation followed the specifications of the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organization for Animal Health (OIE) and the guide ABNT NBR ISO/IEC 17025:2005. The analysis of the 5' NTR region of CSF virus was performed in 145 samples from 29 infected pigs and in 240 samples from 80 pigs originated in the Brazilian CSF-free zone. The tissues tested were spleen, kidney, blood, tonsils, and lymph nodes. Sequencing of the positive samples for 5'NTR region was performed to evaluate the specificity of the RT-qPCR. Tests performed for the RT-qPCR validation demonstrated that the PCR assay was efficient in detecting RNA from CSF virus in all materials from different tissues of infected animals. Furthermore, RNA from CSF virus was not detected in samples of swine originated from the Brazilian CSF-free zone. Hence, it is concluded that RT-qPCR can be used as a complementary diagnostic for CSF. 653 $aClassical Swine Fever 653 $aPCR 653 $aSwine health 700 1 $aFONSECA JÚNIOR, A. A. 700 1 $aOLIVEIRA, A. M. 700 1 $aSALES, É. B. 700 1 $aALVES, B. R. C. 700 1 $aDORELLA, F. A. 700 1 $aCAMARGOS, M. F. 773 $tVeterinary Medicine International, 2014.
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