Data/hora: 16/06/2024 00:24:47

Biblioteca(s): 	Embrapa Agricultura Digital; Embrapa Café; Embrapa Unidades Centrais.	
Data corrente: 	20/04/2010	
Data da última atualização: 	10/03/2011	
Autoria: 	MISSIO, R. F.; CAIXETA, E. T.; ZAMBOLIM, E. M.; ZAMBOLIM, L.; SAKIYAMA, N. S.	
Afiliação: 	ROBSON FERNANDO MISSIO, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA, SAPC; EUNIZE MACIEL ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; NEY SUSSUMU SAKIYAMA, UNIVERSIDADE FEDERAL DE VIÇOSA.	
Título: 	Development and validation of SSR markers for Coffea arabica L.	
Ano de publicação: 	2009	
Fonte/Imprenta: 	In.: CROP BREEDING AND APPLIED BIOTECHNOLOGY. v.9, p.361-371, 2009.	
Idioma: 	Inglês	
Conteúdo: 	With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were more frequent than trinucleotides. Four repeat sequences, (AG/CT)n, (AC/GT)n, (AAG/CTT)n, and (AGG/CCT)n represented 61.1% of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSR markers were validated for further genetic studies of C. arabica.	
Palavras-Chave: 	Biblioteca genômica enriquecida; Coffea arabica L; Coffee; Enriched genomic library; Marcador microssatélite; Molecular marker; SSR marker.	
Thesagro: 	Café; Marcador Molecular.	
Categoria do assunto: 	--
G Melhoramento Genético	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/29283/1/Development-and-validation.pdf	
Registro original: 	Embrapa Café (CNPCa)	
Exemplares: 	CNPTIA - (14829-1) - ADD - AP - PP -  -  -  - DIS
AI-SEDE - (49022-1) - EMB - AP - DD -  -  -  - DIS
CNPCa - (76-1) - UPC - AP - DD -  -  -  - DIS