Data/hora: 12/06/2024 05:44:41

Biblioteca(s): 	Embrapa Gado de Leite.	
Tipo da produção científica: 	Artigo em Periódico Indexado	
Circulação/Nível: 	A - 2	
Data corrente: 	17/04/2019	
Data da última atualização: 	24/01/2023	
Autoria: 	SABINO, Y. N. V.; FOCHAT, R. C.; LIMA, J. C. F.; RIBEIRO, M. T.; ARCURI, P. B.; CARNEIRO, J. da C.; MACHADO, M. A.; REIS, D. R. de L.; MACHADO, A. B. F.; HÚNGARO, H. M.; RIBEIRO, J. B.; PAIVA, A. D.	
Afiliação: 	Yasmin Neves Vieira Sabino, UFJF; Romário Costa Fochat, UFJF; JUNIOR CESAR FERNANDES LIMA, CNPGL; MARLICE TEIXEIRA RIBEIRO, CNPGL; PEDRO BRAGA ARCURI, CNPGL; JAILTON DA COSTA CARNEIRO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; Alessandra Barbosa Ferreira Machado, UFTM, Uberaba; Humberto Moreira Húngaro, UFJF; JOAO BATISTA RIBEIRO, CNPGL; Aline Dias Paiva, UFTM, Uberaba.	
Título: 	Antibacterial activity and lantibiotic post-translational modification genes in Streptococcus spp. isolated from ruminal fluid.	
Ano de publicação: 	2019	
Fonte/Imprenta: 	Annals of Microbiology, v. 69, p. 131-138, 2019.	
DOI: 	10.1007/s13213-018-1407-2	
Idioma: 	Inglês	
Conteúdo: 	Abstract The production of bacteriocins is frequently described in high microbial diversity environments. The aims of this study were to screen Streptococcus spp. isolated from rumen for their antibacterial potential and to determine the presence of post-translational modification genes for lantibiotic class of bacteriocins. The isolates were tested for production of antibacterial compounds by the spot-on-lawn assay. Presence of interfering factors and the sensitivity to proteinase K were evaluated. The ruminal bacteria were identified by 16S rRNA gene sequencing and the subspecific discrimination of the isolates belonging to the same specie was performed by PFGE. The presence of lantibiotic post-translational modification genes (lanB, lanC, and lanM) into bacterial genomes was performed by PCR. The bacteriocin-like inhibitory substances showed broad inhibitory activity and the producer cells were identified as S. equinus, S. lutetiensis, and S. gallolyticus. According to PFGE, the isolates identified as S. equinus belong to different strains. Three ruminal isolates showed at least one of the lantibiotic post-translational modification genes, and lanC was more frequently detected (75%). The production of broad-spectrum bacteriocin-like inhibitory substances by rumen strains suggests that antimicrobial peptides may play an important role in competition in the complex ruminal ecosystem.	
Palavras-Chave: 	Antibacterial activity; Bacteriocin; LanC; Lantibiotic; Streptococcus sp.	
Thesagro: 	Rúmen.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (24612-1) - UPC - AP - DD -  -  -  - DIS