Data/hora: 23/06/2024 13:03:51

Biblioteca(s): | Embrapa Gado de Leite.
Tipo da produção científica: | Artigo em Periódico Indexado
Data corrente: | 10/10/2005
Data da última atualização: | 11/08/2022
Autoria: | CAMARGO, L. S. de A.; VIANA, J. H. M.; SÁ, W. F. de; FERREIRA, A. M.; VALE FILHO, V. R.
Afiliação: | LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL.
Título: | Developmental competence of oocytes from prepubertal Bos indicus crossbred cattle.
Ano de publicação: | 2005
Fonte/Imprenta: | Animal Reproduction Science, v. 85, n. 1/2, p. 53-59, 2005.
DOI: | https://doi.org/10.1016/j.anireprosci.2004.04.020
Idioma: | Inglês
Conteúdo: | The aim of the present study was to evaluate the effect of age on embryogenic competence of oocytes recovered from Bos indicus crossbred calves and heifers. Cumulus-oocyte complexes (COCs) were collected from 4- to 7-month-old calves (experiment 1) and from 9- to 14-month-old heifers (experiment 2) during processing at an abattoir. In both experiments cow COCs were used as control. COCs were in vitro matured and fertilized, and the presumptive zygotes co-cultured with cumulus cells until 224 h post insemination (hpi). In experiment 1, the development rate during the first 68-72 hpi was similar (P > 0.05) between embryos derived from calves and cows. Fewer embryos from calves developed to the blastocyst stage, resulting in a lesser blastocyst production as well as lesser hatching rate (P < 0.05). The embryo development after blastocyst stage was, nevertheless, similar (P > 0.05) between blastocysts derived from calves and cows, suggesting that the development after blastocoele formation is not compromised in embryos derived from calves. In experiment 2, there were no differences (P > 0.05) on cleavage, blastocyst and hatching rates between embryos derived from prepubertal heifers and cows. The rate of blastocyst development until hatching was also similar (P > 0.05). These results indicate that oocytes from 9- to 14-month-old B. indicus crossbred heifers have the same developmental competence as oocytes derived from cows, while ocytes derived from 4- to 7-month-old B. indicus crossbred calves are less competent in developing to the blastocyst stage in vitro. It suggests that oocyte competence in B. indicus crossbred cattle is achieved around 9-14 months of age.
Palavras-Chave: | Fertilização in vitro; Oocitos.
Thesagro: | Bovino.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (14538-1) - UPC - AP - DD -  -  -  - DIS