Data/hora: 06/06/2024 10:16:47

Biblioteca(s): | Embrapa Instrumentação.
Data corrente: | 14/05/2024
Data da última atualização: | 14/05/2024
Autoria: | CORREA, K. C. S.; FACCHINATO, W. M.; HABITZREUTER, F. B.; RIBEIRO, G. H.; RODRIGUES, L. G.; MICOCCI, K. C.; CAMPANA-FILHO, S. P.; COLNAGO, L. A.; SOUZA, D. H. F.
Afiliação: | FEDERAL UNIVERSITY OF SAO CARLOS; UNIVERSITY OF AVEIRO, ST. SANTIAGO; UNIVERSITY OF SAO PAULO; FEDERAL UNIVERSITY OF SAO CARLOS; FEDERAL UNIVERSITY OF SAO CARLOS; UNIVERSITY OF SAO PAULO; LUIZ ALBERTO COLNAGO, CNPDIA; FEDERAL UNIVERSITY OF SAO CARLOS.
Título: | Activity of a Recombinant Chitinase of the Atta sexdens Ant on Different Forms of Chitin and Its Fungicidal Effect against Lasiodiplodia theobromae.
Ano de publicação: | 2024
Fonte/Imprenta: | Polymers, v. 16, 529, 2014.
Páginas: | 1 - 17
DOI: | https:// doi.org/10.3390/polym16040529
Idioma: | Inglês
Conteúdo: | Abstract: This study evaluates the activity of a recombinant chitinase from the leaf-cutting ant Atta sexdens (AsChtII-C4B1) against colloidal and solid α- and β-chitin substrates. 1H NMR analyses of the reaction media showed the formation of N-acetylglucosamine (GlcNAc) as the hydrolysis product. Viscometry analyses revealed a reduction in the viscosity of chitin solutions, indicating that the enzyme decreases their molecular masses. Both solid state 13C NMR and XRD analyses showed minor differences in chitin crystallinity pre- and post-reaction, indicative of partial hydrolysis under the studied conditions, resulting in the formation of GlcNAc and a reduction in molecular mass. However, the enzyme was unable to completely degrade the chitin samples, as they retained most of their solid-state structure. It was also observed that the enzyme acts progressively and with a greater activity on α-chitin than on β-chitin. AsChtII-C4B1 significantly changed the hyphae of the phytopathogenic fungus Lasiodiplodia theobromae, hindering its growth in both solid and liquid media and reducing its dry biomass by approximately 61%. The results demonstrate that AsChtIIC4B1 could be applied as an agent for the bioproduction of chitin derivatives and as a potential antifungal agent.
Palavras-Chave: | Fungicide; Insect chitinase.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1164249/1/P-Activity-of-a-Recombinant-Chitinase-of-the-Atta-sexdens-Ant-on.pdf
Registro original: | Embrapa Instrumentação (CNPDIA)
Exemplares: | CNPDIA - (18423-1) - UPC - AP - DD - PROCI.24/29 -  - 2024/47 - DIS