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| 183. |  | LOPES, G. DE O.; OKINO, C. H.; OLIVEIRA, H. N. DE; OLIVEIRA, M. C. de S. Otimização de PCR para estudo da diversidade genética de Babesia bigemina em amostras de sangue de bezerros da raça Canchim naturalmente infectados. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 13., 2021, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste; Embrapa Instrumentação, 2021. p.29. (Embrapa Pecuária Sudeste. Documentos, 140).| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 187. | | PRATISSOLI, D.; OLIVEIRA, H. N. de; ESPINDULA, M. C.; MAGEVSKI, G. C. Ocorrência da lagarta-da-maçã-do-algodoeiro em frutos de tomateiro no estado do Espírito Santo. Horticultura Brasileira, Brasília, DF, v. 24, n. 2, p. 204-205, abr./jun. 2006.| Biblioteca(s): Embrapa Hortaliças. |
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Título em inglês: Periods of prey absence and stability of the spider mite biological control.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 192. | | MEIRELLES, S. L.; ALENCAR, M. M. de; OLIVEIRA, H. N.; REGITANO, L. C. de A. Estimativas de parâmetros genéticos para características de carcaça da raça Canchim, criados em pastagens. In: REUNION ASOCIACION LATINOAMERICANA DE PRODUCCION ANIMAL, 30.; REUNION ASOCIACION PERUANA DE PRODUCCION ANINAL, 30.; CONGRESSO INTERNACIONAL DE GANDEIRA DE DOBLE PROPOSTO, 5., 2007, Cuzco, Perú. Annales... Cuzco: ALPA: APPA, 2007. 1 CD-ROM. Resumos na seguinte fonte: Archivos Latinoamericano de Produccion Animal, v. 15, (Supl.1), 334, 2007.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 196. | | OLIVEIRA, H. N. de; ANTIGO, M. R.; CARVALHO, G. A.; GLAESER, D. F. Effect of selectivity of herbicides and plant growth regulators used in sugarcane crops on immature stages of Trichogramma galloi (Hymenoptera: Trichogrammatidae). Planta Daninha, Viçosa, MG, v. 32, n. 1, p. 125-131, Jan./Mar 2014.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 199. | | SIMONATO, J.; OLIVEIRA, H. N. de; GRIGOLLI, J. F. J.; LAPS, R. R. Efeito de diferentes formulações de glifosato na viabilidade de ovos de Podisus nigrispinus (Dallas, 1851) (Hemiptera : Pentatomidae). In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapa, 2013. 1 CD-ROM. Editores técnicos: Harley Nonato de Oliveira, Crébio José Avila e Fabrício Fagundes Pereira; SICONBIOL.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
05/11/2020 |
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Data da última atualização: |
05/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LEASTRO, M. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. |
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Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, Universidad Politécnica de Valencia; JESÚS ÁNGEL SÁNCHEZ-NAVARRO, Universidad Politécnica de Valencia. |
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Título: |
Dichorhaviruses Movement Protein and Nucleoprotein Form a Protein Complex That May Be Required for Virus Spread and Interacts in vivo With Viral Movement-Related Cilevirus Proteins. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Microbiology, v.11, November 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Brevipalpus-transmitted viruses (BTVs) belong to the genera Dichorhavirus and Cilevirus and are the main causal agents of the citrus leprosis (CL) disease. In this report, we explored aspects related to the movement mechanism mediated by dichorhaviruses movement proteins (MPs) and the homologous and heterologous interactions among viral proteins related to the movement of citrus leprosis-associated viruses. The membrane-spanning property and topology analysis of the nucleocapsid (N) and MP proteins from two dichorhaviruses revealed that the MPs are proteins tightly associated with the cell membrane, exposing their N- and C-termini to the cytoplasm and the inner part of the nucleus, whereas the N proteins are not membrane-associated. Subcellular localization analysis revealed the presence of dichorhavirus MPs at the cell surface and in the nucleus, while the phosphoproteins (P) were located exclusively in the nucleus and the N proteins in both the cytoplasm and the nucleus. Co-expression analysis with the MP, P, and N proteins showed an interaction network formed between them. We highlight the MP capability to partially redistribute the previously reported N-P core complex, redirecting a portion of the N from the nucleus to the plasmodesmata at the cell periphery, which indicates not only that the MP might guide the intracellular trafficking of the viral infective complex but also that the N protein may be associated with the cell-to-cell movement mechanism of dichorhaviruses. The movement functionality of these MPs was analyzed by using three movement-defective infectious systems. Also, the MP capacity to generate tubular structures on the protoplast surface by ectopic expression was analyzed. Finally, we evaluated the in vivo protein?protein interaction networks between the dichorhavirus MP and/or N proteins with the heterologous cilevirus movement components, which suggest a broad spectrum of interactions, highlighting those among capsid proteins (CP), MPs, and Ns from citrus leprosis-associated viruses. These data may aid in understanding the mixed infection process naturally observed in the field caused by distinct BTVs. Introduction MenosBrevipalpus-transmitted viruses (BTVs) belong to the genera Dichorhavirus and Cilevirus and are the main causal agents of the citrus leprosis (CL) disease. In this report, we explored aspects related to the movement mechanism mediated by dichorhaviruses movement proteins (MPs) and the homologous and heterologous interactions among viral proteins related to the movement of citrus leprosis-associated viruses. The membrane-spanning property and topology analysis of the nucleocapsid (N) and MP proteins from two dichorhaviruses revealed that the MPs are proteins tightly associated with the cell membrane, exposing their N- and C-termini to the cytoplasm and the inner part of the nucleus, whereas the N proteins are not membrane-associated. Subcellular localization analysis revealed the presence of dichorhavirus MPs at the cell surface and in the nucleus, while the phosphoproteins (P) were located exclusively in the nucleus and the N proteins in both the cytoplasm and the nucleus. Co-expression analysis with the MP, P, and N proteins showed an interaction network formed between them. We highlight the MP capability to partially redistribute the previously reported N-P core complex, redirecting a portion of the N from the nucleus to the plasmodesmata at the cell periphery, which indicates not only that the MP might guide the intracellular trafficking of the viral infective complex but also that the N protein may be associated with the cell-to-cell movement mechanism of dichorhaviruses... Mostrar Tudo |
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Thesagro: |
Vírus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/217521/1/2020-Leastro-fmicb-11-571807-Dichorha.pdf
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Marc: |
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100 1 $aLEASTRO, M. O.
245 $aDichorhaviruses Movement Protein and Nucleoprotein Form a Protein Complex That May Be Required for Virus Spread and Interacts in vivo With Viral Movement-Related Cilevirus Proteins.$h[electronic resource]
260 $c2020
520 $aBrevipalpus-transmitted viruses (BTVs) belong to the genera Dichorhavirus and Cilevirus and are the main causal agents of the citrus leprosis (CL) disease. In this report, we explored aspects related to the movement mechanism mediated by dichorhaviruses movement proteins (MPs) and the homologous and heterologous interactions among viral proteins related to the movement of citrus leprosis-associated viruses. The membrane-spanning property and topology analysis of the nucleocapsid (N) and MP proteins from two dichorhaviruses revealed that the MPs are proteins tightly associated with the cell membrane, exposing their N- and C-termini to the cytoplasm and the inner part of the nucleus, whereas the N proteins are not membrane-associated. Subcellular localization analysis revealed the presence of dichorhavirus MPs at the cell surface and in the nucleus, while the phosphoproteins (P) were located exclusively in the nucleus and the N proteins in both the cytoplasm and the nucleus. Co-expression analysis with the MP, P, and N proteins showed an interaction network formed between them. We highlight the MP capability to partially redistribute the previously reported N-P core complex, redirecting a portion of the N from the nucleus to the plasmodesmata at the cell periphery, which indicates not only that the MP might guide the intracellular trafficking of the viral infective complex but also that the N protein may be associated with the cell-to-cell movement mechanism of dichorhaviruses. The movement functionality of these MPs was analyzed by using three movement-defective infectious systems. Also, the MP capacity to generate tubular structures on the protoplast surface by ectopic expression was analyzed. Finally, we evaluated the in vivo protein?protein interaction networks between the dichorhavirus MP and/or N proteins with the heterologous cilevirus movement components, which suggest a broad spectrum of interactions, highlighting those among capsid proteins (CP), MPs, and Ns from citrus leprosis-associated viruses. These data may aid in understanding the mixed infection process naturally observed in the field caused by distinct BTVs. Introduction
650 $aVírus
700 1 $aASTUA, J. de F.
700 1 $aKITAJIMA, E. W.
700 1 $aPALLÁS, V.
700 1 $aSÁNCHEZ-NAVARRO, J. Á.
773 $tFrontiers in Microbiology$gv.11, November 2020.
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