|
|
Registros recuperados : 17 | |
2. | | SÁNCHEZ-NAVARRO, J.; FAJARDO, T. V. M.; ZICCA, S.; PALLÁS, V.; STAVOLONE, L. Caulimoviridae tubule-guided tranport is dictated by movement protein properties. Journal of Virology, Washington, v. 84, n. 8, p. 4109-4112, 2010. 84 4109-4112 Biblioteca(s): Embrapa Uva e Vinho. |
| |
7. | | FIORE, N.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J.; PRODAN, S.; PINO, A. M.; MONTEALEGRE, J. Ilavirus que afectan a los frutales de carozo. Frutícola, Curicó, v. 27, n. 3, p. 89-92, 2006. Biblioteca(s): Embrapa Uva e Vinho. |
| |
9. | | SÁNCHEZ-NAVARRO, J. A.; FIORE, N.; FAJARDO, T. V. M.; PALLÁS, V. Simultaneous detection of the 13 viruses and 5 viroids affecting grapevine by molecular hybridization using a unique probe or 'polyprobe'. In:Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG), 19., 2018, Santiago, Chile. Anais... santiago, Chile: ICGV, April, 2018. p. 40-41. Biblioteca(s): Embrapa Uva e Vinho. |
| |
10. | | FAJARDO, T. V. M.; PEIRÓ, A.; SÁNCHEZ-NAVARRO, J. A.; PALLÁS, V. Role of the 5' untranslated region of the Alfalfa mosaic virus RNA 3 in cell-to-cell and long distance transport. Virología, Madrid, v. 16, n. 2, p. 143-144, 2013. Extraordinario. Edição dos Resumos do 12º Congreso Nacional de Virología, Burgos, Espanha, jun. 2013. Biblioteca(s): Embrapa Uva e Vinho. |
| |
12. | | LEASTRO, M. O.; CASTRO, D. Y. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. Citrus LEPROSIS virus c codifica três proteínas com atividades supressoras de silenciamento gênico Citrus Leprosis Virus C encodes three proteins with gene silencing suppression activity. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 52., 2021. Anais... [Brasília, DF]: Sociedade Brasileira de Fitopatologia, 2021. p. 190. Evento online. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
14. | | FIORE, N. UNES, M. Z.; ZAMORANO, A.; FAJARDO, T. V. M.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. A. Simultaneous detection of the main viruses and viroids affecting grapevine by molecular hybridization using a unique riboprobe or 'Polyprobe'. In: CONGRESS OF THE INTERNATIONAL COUNCIL FOR THE STUDY IF VIRUS AND VIRUS-LIKE DISEASES OF THE GRAPEVINE (ICVG), 17., 2012, Davis, CA. Proceedings... Davis: UCLA, Foundation Plant Services, 2012. p. 146-147. Biblioteca(s): Embrapa Uva e Vinho. |
| |
15. | | LEASTRO, M. O.; VILLAR-ÁLVAREZ, D.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. Spontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. Viruses , v.13, n.12, 2021. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
16. | | APARICIO, F.; FAJARDO, T. V. M.; HERRANZ, M. C.; PEIRÓ, A.; PALLÁS, V.; SANCHEZ-NAVARRO, J. A. The transport of plant viruses: a critical step in the viral cycle. Tropical Plant Pathology, Brasília, DF, v. 36, 2011. p. 1312-1315. 1 CD-ROM. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Mesa redonda 10. Biblioteca(s): Embrapa Uva e Vinho. |
| |
17. | | FIORE, N.; FAJARDO, T. V. M.; PRODAN, S.; HERRANZ, M. C.; APARICIO, F.; MONTEALEGRE, J.; ELENA, S. F.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Genetic diversity of the movement and coat protein genes of South American isolates of Prunus necrotic ringspot virus. Archives of Virology, New York, v. 153, n. 5, p. 909-919, 2008. Biblioteca(s): Embrapa Uva e Vinho. |
| |
Registros recuperados : 17 | |
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
20/01/2022 |
Data da última atualização: |
20/01/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
LEASTRO, M. O.; VILLAR-ÁLVAREZ, D.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. |
Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico, São Paulo; DAVID VILLAR-ÁLVAREZ, CSIC; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, CSIC; JESÚS ÁNGEL SÁNCHEZ-NAVARRO, CSIC. |
Título: |
Spontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Viruses , v.13, n.12, 2021. |
ISSN: |
1999-4915 |
DOI: |
https://doi.org/10.3390/v13122498 |
Idioma: |
Inglês |
Conteúdo: |
Previous results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements. |
Thesagro: |
Vírus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02107naa a2200217 a 4500 001 2139210 005 2022-01-20 008 2021 bl uuuu u00u1 u #d 022 $a1999-4915 024 7 $ahttps://doi.org/10.3390/v13122498$2DOI 100 1 $aLEASTRO, M. O. 245 $aSpontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage.$h[electronic resource] 260 $c2021 520 $aPrevious results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements. 650 $aVírus 700 1 $aVILLAR-ÁLVAREZ, D. 700 1 $aASTUA, J. de F. 700 1 $aKITAJIMA, E. W. 700 1 $aPALLÁS, V. 700 1 $aSÁNCHEZ-NAVARRO, J. Á. 773 $tViruses$gv.13, n.12, 2021.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|