Data/hora: 25/06/2024 13:06:19

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	25/08/2020	
Data da última atualização: 	25/08/2020	
Autoria: 	LEASTRO, M. O.; CASTRO, D. Y. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á.	
Afiliação: 	MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico; DEIBIS YORLENIS ORTEGA CASTRO, Universidad Politécnica de Valencia; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, Universidad Politécnica de Valencia; JESÚS ÁNGEL SÁNCHEZ-NAVARRO, Universidad Politécnica de Valencia.	
Título: 	Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Frontiers in Microbiology, June 2020.	
ISSN: 	1664-302X	
DOI: 	https://doi.org/10.3389/fmicb.2020.01231	
Idioma: 	Inglês	
Conteúdo: 	Citrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting that p61 could also activate a plant defense response mechanism. This is the first report describing the RSS activity for CiLV-C proteins and, moreover, for a member of the family Kitaviridae.	
Thesagro: 	Doença de Planta; Fruta Cítrica.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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